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The expression of ALDH1 in cervical carcinoma.

Yao T, Chen Q, Zhang B, Zhou H, Lin Z - Med. Sci. Monit. (2011)

Bottom Line: Expression of ALDH1 was found in 24.77% of the samples.Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells.In contrast, we found cervical carcinoma had low CD133 population.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT

Background: ALDH1 has been shown to play a role in the early differentiation of stem cells in some human malignancies. Whether cancer stem cells occur in ALDH1-associated cervical cancer is not known.

Material/methods: We tested the hypothesis that cervical carcinomas contain subpopulations of cells that express ALDH1.The following sources of cervical carcinoma tissues were examined for the presence of stem cell marker ALDH1 by immunohistochemistry. Flow cytometric isolation of cancer cells was based on enzymatic activity of ALDH (Aldefluor assay). The mRNA and protein levels of ALDH1 were investigated by qRT-PCR and Western blot, respectively. We also detected the expression of CD133 identified as a stem cell marker for several cancers.

Results: 23/89 samples of invasive squamous carcinoma and 4/20 samples of adenocarcinomas exhibited immunoreactivity to stem cell marker aldehyde dehydrogenase 1 (ALDH1). Expression of ALDH1 was found in 24.77% of the samples. Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells. In contrast, we found cervical carcinoma had low CD133 population.

Conclusions: Cervical carcinoma contains a small subpopulation of cells that may relate to a cancer stem cell-like phenotype, ALDH1.

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Related in: MedlinePlus

Expression of ALDH1 and CD133 on mRNA level.
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f5-medscimonit-17-8-hy21: Expression of ALDH1 and CD133 on mRNA level.

Mentions: ALDH1 mRNA expression was significantly higher in cervical cell lines than was CD133 mRNA expression (Figure 5).


The expression of ALDH1 in cervical carcinoma.

Yao T, Chen Q, Zhang B, Zhou H, Lin Z - Med. Sci. Monit. (2011)

Expression of ALDH1 and CD133 on mRNA level.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539606&req=5

f5-medscimonit-17-8-hy21: Expression of ALDH1 and CD133 on mRNA level.
Mentions: ALDH1 mRNA expression was significantly higher in cervical cell lines than was CD133 mRNA expression (Figure 5).

Bottom Line: Expression of ALDH1 was found in 24.77% of the samples.Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells.In contrast, we found cervical carcinoma had low CD133 population.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT

Background: ALDH1 has been shown to play a role in the early differentiation of stem cells in some human malignancies. Whether cancer stem cells occur in ALDH1-associated cervical cancer is not known.

Material/methods: We tested the hypothesis that cervical carcinomas contain subpopulations of cells that express ALDH1.The following sources of cervical carcinoma tissues were examined for the presence of stem cell marker ALDH1 by immunohistochemistry. Flow cytometric isolation of cancer cells was based on enzymatic activity of ALDH (Aldefluor assay). The mRNA and protein levels of ALDH1 were investigated by qRT-PCR and Western blot, respectively. We also detected the expression of CD133 identified as a stem cell marker for several cancers.

Results: 23/89 samples of invasive squamous carcinoma and 4/20 samples of adenocarcinomas exhibited immunoreactivity to stem cell marker aldehyde dehydrogenase 1 (ALDH1). Expression of ALDH1 was found in 24.77% of the samples. Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells. In contrast, we found cervical carcinoma had low CD133 population.

Conclusions: Cervical carcinoma contains a small subpopulation of cells that may relate to a cancer stem cell-like phenotype, ALDH1.

Show MeSH
Related in: MedlinePlus