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The expression of ALDH1 in cervical carcinoma.

Yao T, Chen Q, Zhang B, Zhou H, Lin Z - Med. Sci. Monit. (2011)

Bottom Line: Expression of ALDH1 was found in 24.77% of the samples.Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells.In contrast, we found cervical carcinoma had low CD133 population.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT

Background: ALDH1 has been shown to play a role in the early differentiation of stem cells in some human malignancies. Whether cancer stem cells occur in ALDH1-associated cervical cancer is not known.

Material/methods: We tested the hypothesis that cervical carcinomas contain subpopulations of cells that express ALDH1.The following sources of cervical carcinoma tissues were examined for the presence of stem cell marker ALDH1 by immunohistochemistry. Flow cytometric isolation of cancer cells was based on enzymatic activity of ALDH (Aldefluor assay). The mRNA and protein levels of ALDH1 were investigated by qRT-PCR and Western blot, respectively. We also detected the expression of CD133 identified as a stem cell marker for several cancers.

Results: 23/89 samples of invasive squamous carcinoma and 4/20 samples of adenocarcinomas exhibited immunoreactivity to stem cell marker aldehyde dehydrogenase 1 (ALDH1). Expression of ALDH1 was found in 24.77% of the samples. Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells. In contrast, we found cervical carcinoma had low CD133 population.

Conclusions: Cervical carcinoma contains a small subpopulation of cells that may relate to a cancer stem cell-like phenotype, ALDH1.

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Immunoreactivity patterns of ALDH1 in cervical squamous carcinomas (A, B), adenocarcinomas (C) and normal epithelium (D). (A) Diffuse positive staining for ALDH1 in cervical squamous carcinomas. (B) Squamous carcinoma cells (<10%) show cytoplasmic staining for ALDH1. (C) Adenocarcinoma cells (<50% but ≥10%) show cytoplasmic staining for ALDH1. (D) ALDH1-negative staining in normal epithelium.
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f1-medscimonit-17-8-hy21: Immunoreactivity patterns of ALDH1 in cervical squamous carcinomas (A, B), adenocarcinomas (C) and normal epithelium (D). (A) Diffuse positive staining for ALDH1 in cervical squamous carcinomas. (B) Squamous carcinoma cells (<10%) show cytoplasmic staining for ALDH1. (C) Adenocarcinoma cells (<50% but ≥10%) show cytoplasmic staining for ALDH1. (D) ALDH1-negative staining in normal epithelium.

Mentions: ALDH1 expression was observed in 23/89 (89 cases) cases of invasive squamous carcinoma and 4/20 cases of adenocarcinoma (20 cases) (Figure 1A–D). No ALDH1 was expressed in normal cervical epithelium obtained from patients undergoing hysterectomy for various non-malignant causes. The majority of positive staining demonstrated focal moderate staining, and occasionally moderate diffuse staining with moderate intensity. Among positive staining cases in primary cervical carcinomas and metastatic carcinomas, staining tended to be diffuse, with a spectrum ranging from weak to moderate-to-strong. The results of immunostaining of the tumor microarrays, organized according to clinicopathologic characteristics of the patients, are shown in Table 1. Correlation was found between the expression of the ALDH1 and lymph nodal metastasis in cervical carcinoma, and patients with recurrent disease were more likely to have a higher ALDH1 expression (Table 1). We also found the expression of ALDH1 has some relationship with Smad3 (Table 2). The immunohistochemical study showed there was almost no CD133-positive area (Figure 2).


The expression of ALDH1 in cervical carcinoma.

Yao T, Chen Q, Zhang B, Zhou H, Lin Z - Med. Sci. Monit. (2011)

Immunoreactivity patterns of ALDH1 in cervical squamous carcinomas (A, B), adenocarcinomas (C) and normal epithelium (D). (A) Diffuse positive staining for ALDH1 in cervical squamous carcinomas. (B) Squamous carcinoma cells (<10%) show cytoplasmic staining for ALDH1. (C) Adenocarcinoma cells (<50% but ≥10%) show cytoplasmic staining for ALDH1. (D) ALDH1-negative staining in normal epithelium.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539606&req=5

f1-medscimonit-17-8-hy21: Immunoreactivity patterns of ALDH1 in cervical squamous carcinomas (A, B), adenocarcinomas (C) and normal epithelium (D). (A) Diffuse positive staining for ALDH1 in cervical squamous carcinomas. (B) Squamous carcinoma cells (<10%) show cytoplasmic staining for ALDH1. (C) Adenocarcinoma cells (<50% but ≥10%) show cytoplasmic staining for ALDH1. (D) ALDH1-negative staining in normal epithelium.
Mentions: ALDH1 expression was observed in 23/89 (89 cases) cases of invasive squamous carcinoma and 4/20 cases of adenocarcinoma (20 cases) (Figure 1A–D). No ALDH1 was expressed in normal cervical epithelium obtained from patients undergoing hysterectomy for various non-malignant causes. The majority of positive staining demonstrated focal moderate staining, and occasionally moderate diffuse staining with moderate intensity. Among positive staining cases in primary cervical carcinomas and metastatic carcinomas, staining tended to be diffuse, with a spectrum ranging from weak to moderate-to-strong. The results of immunostaining of the tumor microarrays, organized according to clinicopathologic characteristics of the patients, are shown in Table 1. Correlation was found between the expression of the ALDH1 and lymph nodal metastasis in cervical carcinoma, and patients with recurrent disease were more likely to have a higher ALDH1 expression (Table 1). We also found the expression of ALDH1 has some relationship with Smad3 (Table 2). The immunohistochemical study showed there was almost no CD133-positive area (Figure 2).

Bottom Line: Expression of ALDH1 was found in 24.77% of the samples.Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells.In contrast, we found cervical carcinoma had low CD133 population.

View Article: PubMed Central - PubMed

Affiliation: Department of Gynecological Oncology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, People's Republic of China.

ABSTRACT

Background: ALDH1 has been shown to play a role in the early differentiation of stem cells in some human malignancies. Whether cancer stem cells occur in ALDH1-associated cervical cancer is not known.

Material/methods: We tested the hypothesis that cervical carcinomas contain subpopulations of cells that express ALDH1.The following sources of cervical carcinoma tissues were examined for the presence of stem cell marker ALDH1 by immunohistochemistry. Flow cytometric isolation of cancer cells was based on enzymatic activity of ALDH (Aldefluor assay). The mRNA and protein levels of ALDH1 were investigated by qRT-PCR and Western blot, respectively. We also detected the expression of CD133 identified as a stem cell marker for several cancers.

Results: 23/89 samples of invasive squamous carcinoma and 4/20 samples of adenocarcinomas exhibited immunoreactivity to stem cell marker aldehyde dehydrogenase 1 (ALDH1). Expression of ALDH1 was found in 24.77% of the samples. Flow cytometric analysis, qRT-PCR and Western blot also confirmed the presence of small subpopulations of ALDH1-positive cells. In contrast, we found cervical carcinoma had low CD133 population.

Conclusions: Cervical carcinoma contains a small subpopulation of cells that may relate to a cancer stem cell-like phenotype, ALDH1.

Show MeSH
Related in: MedlinePlus