Limits...
Current medical research with the application of coupled techniques with mass spectrometry.

Kałużna-Czaplińska J - Med. Sci. Monit. (2011)

Bottom Line: The most effective methods of analysis of organic compounds in biological fluids are coupled chromatographic techniques.Moreover, the identification of potential new compounds, especially new biomarkers in LC-MS, is much more challenging than in GC-MS.The focus is on the description of metabonomics research, strategies for biomarkers identification, medical diagnoses of diseases and research of drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Institute of General and Ecological Chemistry, Technical University of Lodz, Lodz, Poland. jkaluzna@p.lodz.pl

ABSTRACT
The most effective methods of analysis of organic compounds in biological fluids are coupled chromatographic techniques. Capillary gas chromatography/mass spectrometry (GC-MS) allows the most efficient separation, identification and quantification of volatile metabolites in biological fluids. Liquid chromatography-mass spectrometry (LC-MS) is especially suitable for the analysis of non-volatile and/or thermally unstable compounds. A major drawback of liquid chromatography-mass spectrometry is that no standard spectral libraries such as NIST and Wiley for GC-MS are available to facilitate the identification of unknown compounds. Moreover, the identification of potential new compounds, especially new biomarkers in LC-MS, is much more challenging than in GC-MS. Capillary electrophoresis coupled with mass spectrometry (CE-MS) has been widely used to characterize metabolomes. Capillary electrophoresis is a powerful technique for the separation of charged metabolites, offering high analyte resolution. The advantages of CE-MS are applicability for hydrophilic metabolites, robust separation efficiency and short duration of analysis. This review provides an overview of current chromatographic methods--gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry--and their applications in current medical research. The focus is on the description of metabonomics research, strategies for biomarkers identification, medical diagnoses of diseases and research of drugs.

Show MeSH

Related in: MedlinePlus

Bibliographic search in chemical Abstract with the key words GC-MS or LC-MS or CE-MS and in current medical research using Sci Finder Scholar up to December 9th, 2009.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539600&req=5

f1-medscimonit-17-5-ra117: Bibliographic search in chemical Abstract with the key words GC-MS or LC-MS or CE-MS and in current medical research using Sci Finder Scholar up to December 9th, 2009.

Mentions: An increasing interest is anticipated in the near future in chromatographic methods coupled with spectrometry in medical research is not only connected with high resolution but it also improves the limit of detection and potential biomarker identification. Figure 1 shows the frequency of total medical studies with application of GC-MS, LC-MS, and CE-MS methods in the period from 2005 to 2009.


Current medical research with the application of coupled techniques with mass spectrometry.

Kałużna-Czaplińska J - Med. Sci. Monit. (2011)

Bibliographic search in chemical Abstract with the key words GC-MS or LC-MS or CE-MS and in current medical research using Sci Finder Scholar up to December 9th, 2009.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539600&req=5

f1-medscimonit-17-5-ra117: Bibliographic search in chemical Abstract with the key words GC-MS or LC-MS or CE-MS and in current medical research using Sci Finder Scholar up to December 9th, 2009.
Mentions: An increasing interest is anticipated in the near future in chromatographic methods coupled with spectrometry in medical research is not only connected with high resolution but it also improves the limit of detection and potential biomarker identification. Figure 1 shows the frequency of total medical studies with application of GC-MS, LC-MS, and CE-MS methods in the period from 2005 to 2009.

Bottom Line: The most effective methods of analysis of organic compounds in biological fluids are coupled chromatographic techniques.Moreover, the identification of potential new compounds, especially new biomarkers in LC-MS, is much more challenging than in GC-MS.The focus is on the description of metabonomics research, strategies for biomarkers identification, medical diagnoses of diseases and research of drugs.

View Article: PubMed Central - PubMed

Affiliation: Department of Chemistry, Institute of General and Ecological Chemistry, Technical University of Lodz, Lodz, Poland. jkaluzna@p.lodz.pl

ABSTRACT
The most effective methods of analysis of organic compounds in biological fluids are coupled chromatographic techniques. Capillary gas chromatography/mass spectrometry (GC-MS) allows the most efficient separation, identification and quantification of volatile metabolites in biological fluids. Liquid chromatography-mass spectrometry (LC-MS) is especially suitable for the analysis of non-volatile and/or thermally unstable compounds. A major drawback of liquid chromatography-mass spectrometry is that no standard spectral libraries such as NIST and Wiley for GC-MS are available to facilitate the identification of unknown compounds. Moreover, the identification of potential new compounds, especially new biomarkers in LC-MS, is much more challenging than in GC-MS. Capillary electrophoresis coupled with mass spectrometry (CE-MS) has been widely used to characterize metabolomes. Capillary electrophoresis is a powerful technique for the separation of charged metabolites, offering high analyte resolution. The advantages of CE-MS are applicability for hydrophilic metabolites, robust separation efficiency and short duration of analysis. This review provides an overview of current chromatographic methods--gas chromatography-mass spectrometry, liquid chromatography-mass spectrometry and capillary electrophoresis-mass spectrometry--and their applications in current medical research. The focus is on the description of metabonomics research, strategies for biomarkers identification, medical diagnoses of diseases and research of drugs.

Show MeSH
Related in: MedlinePlus