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The inhibitory effect of Zingiber corallinum Hance essential oil on drug-resistant bacteria and evaluation of its acute toxicity.

Yang C, Zhou LL, Wang HY, Huang SN, Liu Q, Hu SL, Li TR, Chen YB, Jiang JX - Med. Sci. Monit. (2011)

Bottom Line: The ZCHO containing 79% terpinen-4-ol revealed better bacteriostatic effect than ZCHO with 34% terpinen-4-ol.ZCHO has obvious bacteriostasis and bactericidal effects, especially against drug-resistant Acinetobacter baumannii.Therefore, ZCHO is a promising natural bioactive component with antibacterial effect and satisfactory safety due to its low toxicity.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, 3rd Military Medical University, Changjiang Zhilu, Daping, Chongqing, P. R. China.

ABSTRACT

Background: The excessive and irregular use of antibiotics could result in the generation and diffusion of drug-resistant bacteria. The aim of this study was to investigate the inhibitory effect of Zingiber corallinum Hance essential oil (ZCHO) on drug-resistant bacteria, especially on drug-resistant Acinetobacter baumannii.

Material/methods: Susceptibility testing was used to evaluate the effect of ZCHO on growth inhibition of drug-resistant bacteria by paper disk method. Mice orally administered with ZCHO were used to observe acute toxicity and to determine median lethal dose (LD₅₀) of ZCHO. Broth dilution method was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ZCHO on drug-resistant Acinetobacter baumannii.

Results: ZCHO exhibited an obvious inhibitory effect not only on gram-negative drug-resistant bacteria including Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Acinetobacter baumannii, but also on gram-positive drug-resistant bacteria including Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus haemolyticus. The ZCHO containing 79% terpinen-4-ol revealed better bacteriostatic effect than ZCHO with 34% terpinen-4-ol. The LD₅₀ of ZCHO was 1790.427 mg/kg. The MIC and MBC of ZCHO on drug-resistant Acinetobacter baumannii were 1457.81 mg/L.

Conclusions: ZCHO has obvious bacteriostasis and bactericidal effects, especially against drug-resistant Acinetobacter baumannii. Therefore, ZCHO is a promising natural bioactive component with antibacterial effect and satisfactory safety due to its low toxicity.

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Related in: MedlinePlus

Pathological changes of different tissues from mice administered with Zingiber corallinum Hance oil. (A–D) representative tissues in the control group, (A) lung tissue, (B) liver tissue, (C) intestinal tract tissue, (D) kidney tissue. (E–H) representative tissues in experimental groups, (E) lung tissue, (F) liver tissue, (G) intestinal tract tissue, (H) kidney tissue. (HE staining, ×100).
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f2-medscimonit-17-5-br139: Pathological changes of different tissues from mice administered with Zingiber corallinum Hance oil. (A–D) representative tissues in the control group, (A) lung tissue, (B) liver tissue, (C) intestinal tract tissue, (D) kidney tissue. (E–H) representative tissues in experimental groups, (E) lung tissue, (F) liver tissue, (G) intestinal tract tissue, (H) kidney tissue. (HE staining, ×100).

Mentions: Through histopathological examination, toxicity lesions in mice treated with ZCHO at high dosage of 2248.53 mg/kg were mainly observed in lung, liver, intestine and kidney tissues. The alveolar space of dead mice had obvious pink fluid and hemorrhage. The alignment of hepatocytes exhibited a slight derangement, with fuzziness of the hepatic cord, sporadic punctiform and lamellar necrosis. A large number of intestinal epithelial cells had degeneration and necrosis. The integrity of villi was destroyed (Figure 2); however, no damage to other organs was observed.


The inhibitory effect of Zingiber corallinum Hance essential oil on drug-resistant bacteria and evaluation of its acute toxicity.

Yang C, Zhou LL, Wang HY, Huang SN, Liu Q, Hu SL, Li TR, Chen YB, Jiang JX - Med. Sci. Monit. (2011)

Pathological changes of different tissues from mice administered with Zingiber corallinum Hance oil. (A–D) representative tissues in the control group, (A) lung tissue, (B) liver tissue, (C) intestinal tract tissue, (D) kidney tissue. (E–H) representative tissues in experimental groups, (E) lung tissue, (F) liver tissue, (G) intestinal tract tissue, (H) kidney tissue. (HE staining, ×100).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539599&req=5

f2-medscimonit-17-5-br139: Pathological changes of different tissues from mice administered with Zingiber corallinum Hance oil. (A–D) representative tissues in the control group, (A) lung tissue, (B) liver tissue, (C) intestinal tract tissue, (D) kidney tissue. (E–H) representative tissues in experimental groups, (E) lung tissue, (F) liver tissue, (G) intestinal tract tissue, (H) kidney tissue. (HE staining, ×100).
Mentions: Through histopathological examination, toxicity lesions in mice treated with ZCHO at high dosage of 2248.53 mg/kg were mainly observed in lung, liver, intestine and kidney tissues. The alveolar space of dead mice had obvious pink fluid and hemorrhage. The alignment of hepatocytes exhibited a slight derangement, with fuzziness of the hepatic cord, sporadic punctiform and lamellar necrosis. A large number of intestinal epithelial cells had degeneration and necrosis. The integrity of villi was destroyed (Figure 2); however, no damage to other organs was observed.

Bottom Line: The ZCHO containing 79% terpinen-4-ol revealed better bacteriostatic effect than ZCHO with 34% terpinen-4-ol.ZCHO has obvious bacteriostasis and bactericidal effects, especially against drug-resistant Acinetobacter baumannii.Therefore, ZCHO is a promising natural bioactive component with antibacterial effect and satisfactory safety due to its low toxicity.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, 3rd Military Medical University, Changjiang Zhilu, Daping, Chongqing, P. R. China.

ABSTRACT

Background: The excessive and irregular use of antibiotics could result in the generation and diffusion of drug-resistant bacteria. The aim of this study was to investigate the inhibitory effect of Zingiber corallinum Hance essential oil (ZCHO) on drug-resistant bacteria, especially on drug-resistant Acinetobacter baumannii.

Material/methods: Susceptibility testing was used to evaluate the effect of ZCHO on growth inhibition of drug-resistant bacteria by paper disk method. Mice orally administered with ZCHO were used to observe acute toxicity and to determine median lethal dose (LD₅₀) of ZCHO. Broth dilution method was used to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ZCHO on drug-resistant Acinetobacter baumannii.

Results: ZCHO exhibited an obvious inhibitory effect not only on gram-negative drug-resistant bacteria including Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae and Acinetobacter baumannii, but also on gram-positive drug-resistant bacteria including Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus haemolyticus. The ZCHO containing 79% terpinen-4-ol revealed better bacteriostatic effect than ZCHO with 34% terpinen-4-ol. The LD₅₀ of ZCHO was 1790.427 mg/kg. The MIC and MBC of ZCHO on drug-resistant Acinetobacter baumannii were 1457.81 mg/L.

Conclusions: ZCHO has obvious bacteriostasis and bactericidal effects, especially against drug-resistant Acinetobacter baumannii. Therefore, ZCHO is a promising natural bioactive component with antibacterial effect and satisfactory safety due to its low toxicity.

Show MeSH
Related in: MedlinePlus