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Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

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Related in: MedlinePlus

The expression of IL-17 was detected by immunochemistry in lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). 400×. Arrow, the positive cells for IL-17.
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f4-medscimonit-17-5-br132: The expression of IL-17 was detected by immunochemistry in lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). 400×. Arrow, the positive cells for IL-17.

Mentions: IL-17 staining was low to moderate intensity in inflammatory cells in HDM alone group (Figure 4B). Abundant infiltrating inflammatory cells were moderate positive for IL-17 in OVA alone group (Figure 4C). In accordance with ELISA and gene mRNA expressions results, IL-17 was strongly positive in inflammatory cells in OVA+HDM group compared with other groups by immunohistochemistry (Figure 4A, D and Table 2)).


Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

The expression of IL-17 was detected by immunochemistry in lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). 400×. Arrow, the positive cells for IL-17.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539583&req=5

f4-medscimonit-17-5-br132: The expression of IL-17 was detected by immunochemistry in lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). 400×. Arrow, the positive cells for IL-17.
Mentions: IL-17 staining was low to moderate intensity in inflammatory cells in HDM alone group (Figure 4B). Abundant infiltrating inflammatory cells were moderate positive for IL-17 in OVA alone group (Figure 4C). In accordance with ELISA and gene mRNA expressions results, IL-17 was strongly positive in inflammatory cells in OVA+HDM group compared with other groups by immunohistochemistry (Figure 4A, D and Table 2)).

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

Show MeSH
Related in: MedlinePlus