Limits...
Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

Show MeSH

Related in: MedlinePlus

The levels of Th1, Th2 and Th17 related cytokines were detected in serum (A) and bronchoalveolar lavage fluid (B). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysis.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539583&req=5

f3-medscimonit-17-5-br132: The levels of Th1, Th2 and Th17 related cytokines were detected in serum (A) and bronchoalveolar lavage fluid (B). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysis.

Mentions: Th2 and Th17 response related cytokines in serum and bronchoalveolar lavage fluid were detected by ELISA. In serum, IL-17 was sharply up-regulated only in OVA+HDM group compared with other groups, while INF-γ and IL-23 showed no evident increase (Figure 3A). Both IL-17 and IL-23 were apparently increased in bronchoalveolar lavage fluid in OVA+HDM group, but not in HDM alone group and OVA alone group (Figure 3B). IL-5 was augmented in both OVA alone group and OVA+HDM group in serum and bronchoalveolar lavage fluid compared to control group (Figure 3A, B). In comparison with OVA alone group, IL-5 was increased with no significant difference in OVA+HDM group in bronchoalveolar lavage fluid (Figure 3A). In other words, Th2 relative response increased in OVA alone group not in OVA+HDM group.


Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

The levels of Th1, Th2 and Th17 related cytokines were detected in serum (A) and bronchoalveolar lavage fluid (B). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539583&req=5

f3-medscimonit-17-5-br132: The levels of Th1, Th2 and Th17 related cytokines were detected in serum (A) and bronchoalveolar lavage fluid (B). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysis.
Mentions: Th2 and Th17 response related cytokines in serum and bronchoalveolar lavage fluid were detected by ELISA. In serum, IL-17 was sharply up-regulated only in OVA+HDM group compared with other groups, while INF-γ and IL-23 showed no evident increase (Figure 3A). Both IL-17 and IL-23 were apparently increased in bronchoalveolar lavage fluid in OVA+HDM group, but not in HDM alone group and OVA alone group (Figure 3B). IL-5 was augmented in both OVA alone group and OVA+HDM group in serum and bronchoalveolar lavage fluid compared to control group (Figure 3A, B). In comparison with OVA alone group, IL-5 was increased with no significant difference in OVA+HDM group in bronchoalveolar lavage fluid (Figure 3A). In other words, Th2 relative response increased in OVA alone group not in OVA+HDM group.

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

Show MeSH
Related in: MedlinePlus