Limits...
Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

Show MeSH

Related in: MedlinePlus

The expressions of RORc, IL-17A, IL-23 and GATA-3 gene mRNA were detected in each group in spleen, thymus and lung tissue.spleen tissue (A), thymus tissue (B) and in lung tissue (C). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysi).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539583&req=5

f2-medscimonit-17-5-br132: The expressions of RORc, IL-17A, IL-23 and GATA-3 gene mRNA were detected in each group in spleen, thymus and lung tissue.spleen tissue (A), thymus tissue (B) and in lung tissue (C). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysi).

Mentions: In spleen tissue, RORc, IL-17, IL-23 and GATA3 mRNA expressions were increased in OVA+HDM group, while they were elevated with no significant difference in HDM alone and OVA alone group in comparison with control group (Figure 2A). In thymus tissue, there was no significant difference in gene mRNA expressions among all the groups. (Figure 2B). Th2 and Th17 response in spleen tissue was more obvious than that in thymus tissue. We deduced that spleen tissue was easy respone to the stimulation of allergen. In lung tissue, Th2 and Th17 related gene mRNA expressions were not significant increased in HDM alone group as we supposed. They were significant elevated in OVA alone group and OVA+HDM group compared with control group. In comparison with OVA alone group, Th2 and Th17 responses were still significant augmented in OVA+HDM group. (Figure 2C). Th2 and Th17 response related gene mRNA expressions were augmented when OVA-induced asthmatic mice were exposed to HDM.


Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

The expressions of RORc, IL-17A, IL-23 and GATA-3 gene mRNA were detected in each group in spleen, thymus and lung tissue.spleen tissue (A), thymus tissue (B) and in lung tissue (C). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysi).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539583&req=5

f2-medscimonit-17-5-br132: The expressions of RORc, IL-17A, IL-23 and GATA-3 gene mRNA were detected in each group in spleen, thymus and lung tissue.spleen tissue (A), thymus tissue (B) and in lung tissue (C). * P<0.05, Statistical differences were compared to control group by two-way ANOVA test and post-hoc pairwise analysis, # P<0.05, Statistical differences were compared to OVA alone group by two-way ANOVA test and post-hoc pairwise analysi).
Mentions: In spleen tissue, RORc, IL-17, IL-23 and GATA3 mRNA expressions were increased in OVA+HDM group, while they were elevated with no significant difference in HDM alone and OVA alone group in comparison with control group (Figure 2A). In thymus tissue, there was no significant difference in gene mRNA expressions among all the groups. (Figure 2B). Th2 and Th17 response in spleen tissue was more obvious than that in thymus tissue. We deduced that spleen tissue was easy respone to the stimulation of allergen. In lung tissue, Th2 and Th17 related gene mRNA expressions were not significant increased in HDM alone group as we supposed. They were significant elevated in OVA alone group and OVA+HDM group compared with control group. In comparison with OVA alone group, Th2 and Th17 responses were still significant augmented in OVA+HDM group. (Figure 2C). Th2 and Th17 response related gene mRNA expressions were augmented when OVA-induced asthmatic mice were exposed to HDM.

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

Show MeSH
Related in: MedlinePlus