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Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

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Related in: MedlinePlus

HE staining of paraffin-embedded lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). Magnification was all 400×.
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f1-medscimonit-17-5-br132: HE staining of paraffin-embedded lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). Magnification was all 400×.

Mentions: Eosinophil infiltrations, epithelial cell hyperplasia and airway obstruction were evident in each experimental group in comparison with control group. More inflammatory cell infiltration was observed in HDM alone group than that in control group (Figure 1A, B). It indicated that HDM can directly evoke non-allergic inflammatory response, in accordance with others’ conclusions [14]. Compared with OVA alone group, the inflammatory response in OVA+HDM group was more evident (Figure 1 C, D). HDM can evoke non-allergic inflammatory, we assume that the non-allergic inflammatory is present in OVA+HDM group.


Th17 response is augmented in OVA-induced asthmatic mice exposed to HDM.

Lan F, Liu K, Zhang J, Qi Y, Li K, Lin P - Med. Sci. Monit. (2011)

HE staining of paraffin-embedded lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). Magnification was all 400×.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539583&req=5

f1-medscimonit-17-5-br132: HE staining of paraffin-embedded lung tissue in control group (A), HDM alone group (B), OVA alone group (C) and OVA+HDM group (D). Magnification was all 400×.
Mentions: Eosinophil infiltrations, epithelial cell hyperplasia and airway obstruction were evident in each experimental group in comparison with control group. More inflammatory cell infiltration was observed in HDM alone group than that in control group (Figure 1A, B). It indicated that HDM can directly evoke non-allergic inflammatory response, in accordance with others’ conclusions [14]. Compared with OVA alone group, the inflammatory response in OVA+HDM group was more evident (Figure 1 C, D). HDM can evoke non-allergic inflammatory, we assume that the non-allergic inflammatory is present in OVA+HDM group.

Bottom Line: Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group.However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

View Article: PubMed Central - PubMed

Affiliation: Division of Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, PR China.

ABSTRACT

Background: It is widely accepted that T helper 2 (Th2) cells, Th17 cells and their cytokines orchestrate the feature of asthma. However, most of studies on asthma mechanisms use a single allergen challenge model. Actually, humans are concurrently exposed to various allergens, and the mechanism of asthma with complex allergen exposure is less well defined. To explore whether the mechanism would be altered if asthma patients are re-exposed to another allergen, we exposed the chicken egg albumin (OVA) induced-asthmatic mice to house dust mite (HDM).

Material/methods: HE staining was used to analyze pathologic variation in lung tissue of mice in each sub-group: control group, HDM alone group, OVA alone group and OVA+HDM group. Th1, Th2 and Th17 associated gene mRNA expressions were detected by quantitative PCR; associated cytokines were determined by ELISA or immunohistochemistry.

Results: The severe of inflammatory cell infiltration, the augmentation of Th17 and Th2 related gene mRNA expressions and the increase of Th17 associated cytokines expression were shown in OVA+HDM group in comparison with OVA alone group. However, Th2 related cytokines were increased with no significant difference in OVA+HDM group compared with OVA alone group.

Conclusions: We have found that Th17 response is connected with inflammation in the OVA-induced asthmatic mice exposed to HDM. When OVA-induced asthmatic mice are re-exposed to HDM, the pathomechanism is different from OVA alone exposure. HDM, indoor allergen, may be an important interferential factor for asthma therapy. It will give an important direction in the development of future asthma therapy.

Show MeSH
Related in: MedlinePlus