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Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S.

Žilinskas J, Žekonis J, Žekonis G, Šadzevičienė R, Sapragonienė M, Navickaitė J, Barzdžiukaitė I - Med. Sci. Monit. (2011)

Bottom Line: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated.Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects.Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence.

View Article: PubMed Central - PubMed

Affiliation: STOMATOLOGIJA Baltic Dental and Maxillofacial Journal, Kaunas, Lithuania.

ABSTRACT

Background: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.

Material/methods: The study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).

Results: Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.

Conclusions: This study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.

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Related in: MedlinePlus

Luminol-dependent chemiluminescence of 1×106 polymorphonuclear leukocytes stimulated by unopsonized E. coli and polymorphonuclear leukocytes stimulated by unopsonized E. coli and affected by Traumeel S. *p>0.05 – between all groups 0–20 min; – between Periodontitis group stimulated PMN and Periodontitis group stimulated PMN+Traumeel S at all time points; – between Control group stimulated PMN and Control group stimulated PMN+Traumeel S at all time points. p<0.01 – between both Control groups and both Periodontitis groups (30–120 min).
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f3-medscimonit-17-5-cr284: Luminol-dependent chemiluminescence of 1×106 polymorphonuclear leukocytes stimulated by unopsonized E. coli and polymorphonuclear leukocytes stimulated by unopsonized E. coli and affected by Traumeel S. *p>0.05 – between all groups 0–20 min; – between Periodontitis group stimulated PMN and Periodontitis group stimulated PMN+Traumeel S at all time points; – between Control group stimulated PMN and Control group stimulated PMN+Traumeel S at all time points. p<0.01 – between both Control groups and both Periodontitis groups (30–120 min).

Mentions: Stimulation of both patient and control neutrophils with unopsonized E. coli induced significantly increased luminol- and lucigenin-dependent chemiluminescence (p<0.01 at most time points). The level of luminol-dependent chemiluminescence of the control group neutrophils induced by unopsonized E. coli was higher (p<0.01 (30–120 min.)), compared to those in periodontitis patients (Figure 3), whereas lucigenin-dependent CL of stimulated periodontitis patient neutrophils produced significantly higher (p<0.01 at all time points) light output than those in controls (Figure 4).


Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S.

Žilinskas J, Žekonis J, Žekonis G, Šadzevičienė R, Sapragonienė M, Navickaitė J, Barzdžiukaitė I - Med. Sci. Monit. (2011)

Luminol-dependent chemiluminescence of 1×106 polymorphonuclear leukocytes stimulated by unopsonized E. coli and polymorphonuclear leukocytes stimulated by unopsonized E. coli and affected by Traumeel S. *p>0.05 – between all groups 0–20 min; – between Periodontitis group stimulated PMN and Periodontitis group stimulated PMN+Traumeel S at all time points; – between Control group stimulated PMN and Control group stimulated PMN+Traumeel S at all time points. p<0.01 – between both Control groups and both Periodontitis groups (30–120 min).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539582&req=5

f3-medscimonit-17-5-cr284: Luminol-dependent chemiluminescence of 1×106 polymorphonuclear leukocytes stimulated by unopsonized E. coli and polymorphonuclear leukocytes stimulated by unopsonized E. coli and affected by Traumeel S. *p>0.05 – between all groups 0–20 min; – between Periodontitis group stimulated PMN and Periodontitis group stimulated PMN+Traumeel S at all time points; – between Control group stimulated PMN and Control group stimulated PMN+Traumeel S at all time points. p<0.01 – between both Control groups and both Periodontitis groups (30–120 min).
Mentions: Stimulation of both patient and control neutrophils with unopsonized E. coli induced significantly increased luminol- and lucigenin-dependent chemiluminescence (p<0.01 at most time points). The level of luminol-dependent chemiluminescence of the control group neutrophils induced by unopsonized E. coli was higher (p<0.01 (30–120 min.)), compared to those in periodontitis patients (Figure 3), whereas lucigenin-dependent CL of stimulated periodontitis patient neutrophils produced significantly higher (p<0.01 at all time points) light output than those in controls (Figure 4).

Bottom Line: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated.Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects.Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence.

View Article: PubMed Central - PubMed

Affiliation: STOMATOLOGIJA Baltic Dental and Maxillofacial Journal, Kaunas, Lithuania.

ABSTRACT

Background: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.

Material/methods: The study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).

Results: Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.

Conclusions: This study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.

Show MeSH
Related in: MedlinePlus