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Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S.

Žilinskas J, Žekonis J, Žekonis G, Šadzevičienė R, Sapragonienė M, Navickaitė J, Barzdžiukaitė I - Med. Sci. Monit. (2011)

Bottom Line: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated.Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects.Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence.

View Article: PubMed Central - PubMed

Affiliation: STOMATOLOGIJA Baltic Dental and Maxillofacial Journal, Kaunas, Lithuania.

ABSTRACT

Background: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.

Material/methods: The study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).

Results: Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.

Conclusions: This study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.

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Lucigenin-dependent chemiluminescence of 1×106 unstimulated polymorphonuclear leukocytes and unstimulated polymorphonuclear leukocytes affected by Traumeel S. * p>0.05 – between Control group unstimulated PMN and Control group unstimulated PMN+Traumeel S at all time points. p<0.01 – between Periodontitis group unstimulated PMN and Periodontitis group unstimulated PMN+Traumeel S at all time points; – between Periodontitis group unstimulated PMN and both Control groups at all time points; – between Periodontitis group unstimulated PMN+Traumeel S and both Control groups at all time points.
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f1-medscimonit-17-5-cr284: Lucigenin-dependent chemiluminescence of 1×106 unstimulated polymorphonuclear leukocytes and unstimulated polymorphonuclear leukocytes affected by Traumeel S. * p>0.05 – between Control group unstimulated PMN and Control group unstimulated PMN+Traumeel S at all time points. p<0.01 – between Periodontitis group unstimulated PMN and Periodontitis group unstimulated PMN+Traumeel S at all time points; – between Periodontitis group unstimulated PMN and both Control groups at all time points; – between Periodontitis group unstimulated PMN+Traumeel S and both Control groups at all time points.

Mentions: The measurement of neutrophil-burst was performed in 2 venous blood assays of periodontitis patients and controls. In the first assay, the neutrophil-burst was measured by chemiluminescence through monitoring the O2−-induced oxidation of lucigenin. Unstimulated neutrophils taken from patients produced higher levels (p<0.01 at all time points) of light output (Figure 1), compared to those from controls. Pre-incubation of unstimulated neutrophils of patients with Traumeel S in the patient group significantly (p<0.01 at all time points) inhibited light output from the baseline lucigenin-dependent chemiluminescence, while this had no effect (p>0.05 at all time points) on analogous neutrophils of the control group (Figure 1).


Inhibition of peripheral blood neutrophil oxidative burst in periodontitis patients with a homeopathic medication Traumeel S.

Žilinskas J, Žekonis J, Žekonis G, Šadzevičienė R, Sapragonienė M, Navickaitė J, Barzdžiukaitė I - Med. Sci. Monit. (2011)

Lucigenin-dependent chemiluminescence of 1×106 unstimulated polymorphonuclear leukocytes and unstimulated polymorphonuclear leukocytes affected by Traumeel S. * p>0.05 – between Control group unstimulated PMN and Control group unstimulated PMN+Traumeel S at all time points. p<0.01 – between Periodontitis group unstimulated PMN and Periodontitis group unstimulated PMN+Traumeel S at all time points; – between Periodontitis group unstimulated PMN and both Control groups at all time points; – between Periodontitis group unstimulated PMN+Traumeel S and both Control groups at all time points.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539582&req=5

f1-medscimonit-17-5-cr284: Lucigenin-dependent chemiluminescence of 1×106 unstimulated polymorphonuclear leukocytes and unstimulated polymorphonuclear leukocytes affected by Traumeel S. * p>0.05 – between Control group unstimulated PMN and Control group unstimulated PMN+Traumeel S at all time points. p<0.01 – between Periodontitis group unstimulated PMN and Periodontitis group unstimulated PMN+Traumeel S at all time points; – between Periodontitis group unstimulated PMN and both Control groups at all time points; – between Periodontitis group unstimulated PMN+Traumeel S and both Control groups at all time points.
Mentions: The measurement of neutrophil-burst was performed in 2 venous blood assays of periodontitis patients and controls. In the first assay, the neutrophil-burst was measured by chemiluminescence through monitoring the O2−-induced oxidation of lucigenin. Unstimulated neutrophils taken from patients produced higher levels (p<0.01 at all time points) of light output (Figure 1), compared to those from controls. Pre-incubation of unstimulated neutrophils of patients with Traumeel S in the patient group significantly (p<0.01 at all time points) inhibited light output from the baseline lucigenin-dependent chemiluminescence, while this had no effect (p>0.05 at all time points) on analogous neutrophils of the control group (Figure 1).

Bottom Line: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated.Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects.Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence.

View Article: PubMed Central - PubMed

Affiliation: STOMATOLOGIJA Baltic Dental and Maxillofacial Journal, Kaunas, Lithuania.

ABSTRACT

Background: The anti-inflammatory effects of a homeopathic remedy, Traumeel S, have been observed in experimental and clinical studies; however, its antioxidant properties have not been elucidated. The aim of the present study was to evaluate the antioxidant effects of Traumeel S on peripheral blood neutrophils in patients with periodontitis.

Material/methods: The study was performed using venous blood of 22 individuals with chronic periodontitis and 21 healthy subjects. The antioxidant effects of Traumeel S on the production of reactive oxygen species by unstimulated and stimulated with unopsonized E. coli neutrophils were investigated using luminol- and lucigenin-dependent chemiluminescence (CL).

Results: Polymorphonuclear leukocytes of periodontitis patients produced higher levels (p<0.01) of light output of lucigenin-dependent chemiluminescence and significantly reduced (p<0.01) light output of luminol-dependent chemiluminescence than analogous cells of healthy subjects. Highly diluted (10⁻⁴ of the stem solution) Traumeel S significantly (by approximately 50%) reduced superoxide-induced oxidation of lucigenin by unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of periodontitis patients and had a tendency to intensify luminol-dependent chemiluminescence. Preincubation of the unstimulated and stimulated with unopsonized E. coli polymorphonuclear leukocytes of healthy subjects with Traumeel S exerts no inhibitory action on the luminol- and lucigenin-dependent chemiluminescence of the above-mentioned cells.

Conclusions: This study indicates that Traumeel S may significantly reduce production of superoxide anion by unstimulated and stimulated peripheral blood polymorphonuclear neutrophils of periodontitis patients.

Show MeSH
Related in: MedlinePlus