Limits...
Phage therapy of Cronobacter-induced urinary tract infection in mice.

Tóthová L, Celec P, Bábíčková J, Gajdošová J, Al-Alami H, Kamodyova N, Drahovská H, Liptáková A, Turňa J, Hodosy J - Med. Sci. Monit. (2011)

Bottom Line: Phage therapy reduced the number of Cronobacter colonies in the kidney by 70%.Higher levels of malondialdehyde were reduced by phage therapy without affecting the antioxidant status.Long-term effects and safety of the treatment are currently unknown.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia.

ABSTRACT

Background: Cronobacter spp. is an opportunistic pathogen causing rare but dangerous cases of meningitis, sepsis and urinary tract infection. Phage therapy overcomes antibiotic resistance and represents an alternative approach to standard antimicrobial treatment. There are no published studies on the use of phages against Cronobacter spp. in vivo. The aim of our study was to prove the effects of isolated Cronobacter-specific phages on renal colonization in a model of urinary tract infection in mice.

Material/methods: Urinary tract infection was induced by transurethral application of Cronobacter turicensis (1011 CFU/ml). Simultaneously, isolated Cronobacter-specific phages were administered intraperitoneally (1011 PFU/ml). After 24 hours, kidneys and bladder were collected and used for cultivation and analysis of gene expression and oxidative stress markers.

Results: Phage therapy reduced the number of Cronobacter colonies in the kidney by 70%. Higher levels of malondialdehyde were reduced by phage therapy without affecting the antioxidant status. The expression of pro-inflammatory cytokines tumor necrosis factor-alpha and monocyte chemoattractant protein-1 increased by the infection and was attenuated by phage therapy.

Conclusions: Phage therapy proved effective in the prevention of ascending renal infection in a murine model of urinary tract infection. Long-term effects and safety of the treatment are currently unknown. Further studies should test phage therapy in other Cronobacter infection models.

Show MeSH

Related in: MedlinePlus

Cultivation analysis of Cronobacter colonies and phage plaques recovered from the kidney and from the bladder. * denotes p<0.01 vs. CRONO.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539570&req=5

f1-medscimonit-17-7-br173: Cultivation analysis of Cronobacter colonies and phage plaques recovered from the kidney and from the bladder. * denotes p<0.01 vs. CRONO.

Mentions: Cultivation analysis revealed that bacterial colonies were found only in bladders and kidneys of mice that were injected with Cronobacter cells. Mean bacterial count in the kidney was 734 cfu and phage therapy decreased the count by 70% (Z=3.06; p=0.002, Figure 1A). In the bladder the mean bacterial load was cca 900 cfu in both CRONO and CRONO + PHAGE groups (Z=−0.55; p=0.58; Figure 1B). No significant differences were found between the PHAGE and CRONO + PHAGE groups in the number of phage plaques in the kidney (Z=0.32; p=0.75; Figure 1C), but were higher in the CRONO + PHAGE group, although not significant due to high interindividual variability (Z=−1.53; p=0.13; Figure 1D).


Phage therapy of Cronobacter-induced urinary tract infection in mice.

Tóthová L, Celec P, Bábíčková J, Gajdošová J, Al-Alami H, Kamodyova N, Drahovská H, Liptáková A, Turňa J, Hodosy J - Med. Sci. Monit. (2011)

Cultivation analysis of Cronobacter colonies and phage plaques recovered from the kidney and from the bladder. * denotes p<0.01 vs. CRONO.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539570&req=5

f1-medscimonit-17-7-br173: Cultivation analysis of Cronobacter colonies and phage plaques recovered from the kidney and from the bladder. * denotes p<0.01 vs. CRONO.
Mentions: Cultivation analysis revealed that bacterial colonies were found only in bladders and kidneys of mice that were injected with Cronobacter cells. Mean bacterial count in the kidney was 734 cfu and phage therapy decreased the count by 70% (Z=3.06; p=0.002, Figure 1A). In the bladder the mean bacterial load was cca 900 cfu in both CRONO and CRONO + PHAGE groups (Z=−0.55; p=0.58; Figure 1B). No significant differences were found between the PHAGE and CRONO + PHAGE groups in the number of phage plaques in the kidney (Z=0.32; p=0.75; Figure 1C), but were higher in the CRONO + PHAGE group, although not significant due to high interindividual variability (Z=−1.53; p=0.13; Figure 1D).

Bottom Line: Phage therapy reduced the number of Cronobacter colonies in the kidney by 70%.Higher levels of malondialdehyde were reduced by phage therapy without affecting the antioxidant status.Long-term effects and safety of the treatment are currently unknown.

View Article: PubMed Central - PubMed

Affiliation: Institute of Molecular Biomedicine, Comenius University, Bratislava, Slovakia.

ABSTRACT

Background: Cronobacter spp. is an opportunistic pathogen causing rare but dangerous cases of meningitis, sepsis and urinary tract infection. Phage therapy overcomes antibiotic resistance and represents an alternative approach to standard antimicrobial treatment. There are no published studies on the use of phages against Cronobacter spp. in vivo. The aim of our study was to prove the effects of isolated Cronobacter-specific phages on renal colonization in a model of urinary tract infection in mice.

Material/methods: Urinary tract infection was induced by transurethral application of Cronobacter turicensis (1011 CFU/ml). Simultaneously, isolated Cronobacter-specific phages were administered intraperitoneally (1011 PFU/ml). After 24 hours, kidneys and bladder were collected and used for cultivation and analysis of gene expression and oxidative stress markers.

Results: Phage therapy reduced the number of Cronobacter colonies in the kidney by 70%. Higher levels of malondialdehyde were reduced by phage therapy without affecting the antioxidant status. The expression of pro-inflammatory cytokines tumor necrosis factor-alpha and monocyte chemoattractant protein-1 increased by the infection and was attenuated by phage therapy.

Conclusions: Phage therapy proved effective in the prevention of ascending renal infection in a murine model of urinary tract infection. Long-term effects and safety of the treatment are currently unknown. Further studies should test phage therapy in other Cronobacter infection models.

Show MeSH
Related in: MedlinePlus