Limits...
Gastric pH and motility in a porcine model of acute lung injury using a wireless motility capsule.

Rauch S, Muellenbach RM, Johannes A, Zollhöfer B, Roewer N - Med. Sci. Monit. (2011)

Bottom Line: Gastric pH values were statistically significantly different (P<0.003) in the animals over time and ranged from 1.15 to 9.94 [5.73 ± 0.47 (mean ± SD)] with an interquartile range of 0.11 to 2.07.The capsule pressure recordings ranged from 2 to 4 mmHg [2.6 ± 0.5 mmHg (mean ± SD)].There was no change in pressure patterns or sudden rise of pH >3 pH units during 24 hours.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, University of Wurzburg, Wurzburg, Germany. rauch_s@klinik.uni-wuerzburg.de

ABSTRACT

Background: Evaluation of gastric pH and motility in a porcine model of acute lung injury using a novel, wireless motility capsule.

Material/methods: A motility capsule was applied into the stomach of 7 Pietrain pigs with acute lung injury induced by high volume saline lavage. Wireless transmission of pH, pressure and temperature data was performed by a recorder attached to the animal's abdomen. Gastric motility was evaluated using pH and pressure values, and capsule location was confirmed by autopsy.

Results: Gastric pH values were statistically significantly different (P<0.003) in the animals over time and ranged from 1.15 to 9.94 [5.73 ± 0.47 (mean ± SD)] with an interquartile range of 0.11 to 2.07. The capsule pressure recordings ranged from 2 to 4 mmHg [2.6 ± 0.5 mmHg (mean ± SD)]. There was no change in pressure patterns or sudden rise of pH >3 pH units during 24 hours. All animals had a gastroparesis with the capsules located in the stomach as indicated by the pressure and pH data and confirmed by necropsy.

Conclusions: The preliminary data show that Pietrain pigs with acute lung injury have a high variability in gastric pH and severely disturbed gastric motility.

Show MeSH

Related in: MedlinePlus

SmartPill pH, pressure and temperature tracing in one pig over 24 hours. The pH tracing is shown in green, the pressure tracing in red and the temperature in blue.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539567&req=5

f2-medscimonit-17-7-br161: SmartPill pH, pressure and temperature tracing in one pig over 24 hours. The pH tracing is shown in green, the pressure tracing in red and the temperature in blue.

Mentions: The experiment was part of a study investigating different ventilation strategies in a large animal model of ARDS that has been validated and recently published [10]. Experiments were performed on 7 healthy female Pietrain pigs (53.7±3.9 kg) over a 24-hour period. Shortly after intramuscular premedication with ketamine (10 mg/kg), an intravenous line was obtained and the animals were anesthetized with continuous infusion of 5–10 mg/kg thiopental and 0.01 mg/kg/h fentanyl throughout the experiment. Neuromuscular block was achieved by continuous infusion of 0.1 mg/kg/h pancuronium. The trachea was intubated with a cuffed 8.5 mm ID endotracheal tube with an additional side lumen ending at the tip (Rueschelit®, Ruesch, Kernen, Germany). Severe ARDS was induced by bilateral pulmonary lavages with 30 mL/kg isotonic saline (38°C), repeated every 10 minutes until PaO2 decreased to less than 60 mmHg and remained stable for 60 minutes with unchanged ventilator settings. On average, 7±2 lavages with approximately 12 L saline per animal were necessary for ARDS induction. The lungs were ventilated with high-frequency oscillation. A pH, pressure and temperature sensing capsule (SmartPill™, SmartPill Corp., Buffalo, NY) was positioned endoscopically with a capsule delivery device (AdvanCE™, US Endoscopy, Mentor, OH) into the stomach. The capsule data were transmitted to a recorder attached to the abdomen. MotiliGI software (MotiliGI 1.3.1, SmartPill, Inc.) (Figure 2) was used to calculate gastric emptying of the capsule by measuring the time required for the pH to change from the acidic stomach to the alkaline duodenum, as well as a change in pressure patterns prior to emptying. Gastroparesis was defined by a gastric emptying time exceeding 5 hours [11]. Pressure patterns are characterized as mean peak amplitude and mean contractions per minute. Mean peak amplitude is the quotient of the sum of amplitudes divided by the number of contractions. The location of the capsule was confirmed by autopsy after the animals were sacrificed after 24 hours.


Gastric pH and motility in a porcine model of acute lung injury using a wireless motility capsule.

Rauch S, Muellenbach RM, Johannes A, Zollhöfer B, Roewer N - Med. Sci. Monit. (2011)

SmartPill pH, pressure and temperature tracing in one pig over 24 hours. The pH tracing is shown in green, the pressure tracing in red and the temperature in blue.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539567&req=5

f2-medscimonit-17-7-br161: SmartPill pH, pressure and temperature tracing in one pig over 24 hours. The pH tracing is shown in green, the pressure tracing in red and the temperature in blue.
Mentions: The experiment was part of a study investigating different ventilation strategies in a large animal model of ARDS that has been validated and recently published [10]. Experiments were performed on 7 healthy female Pietrain pigs (53.7±3.9 kg) over a 24-hour period. Shortly after intramuscular premedication with ketamine (10 mg/kg), an intravenous line was obtained and the animals were anesthetized with continuous infusion of 5–10 mg/kg thiopental and 0.01 mg/kg/h fentanyl throughout the experiment. Neuromuscular block was achieved by continuous infusion of 0.1 mg/kg/h pancuronium. The trachea was intubated with a cuffed 8.5 mm ID endotracheal tube with an additional side lumen ending at the tip (Rueschelit®, Ruesch, Kernen, Germany). Severe ARDS was induced by bilateral pulmonary lavages with 30 mL/kg isotonic saline (38°C), repeated every 10 minutes until PaO2 decreased to less than 60 mmHg and remained stable for 60 minutes with unchanged ventilator settings. On average, 7±2 lavages with approximately 12 L saline per animal were necessary for ARDS induction. The lungs were ventilated with high-frequency oscillation. A pH, pressure and temperature sensing capsule (SmartPill™, SmartPill Corp., Buffalo, NY) was positioned endoscopically with a capsule delivery device (AdvanCE™, US Endoscopy, Mentor, OH) into the stomach. The capsule data were transmitted to a recorder attached to the abdomen. MotiliGI software (MotiliGI 1.3.1, SmartPill, Inc.) (Figure 2) was used to calculate gastric emptying of the capsule by measuring the time required for the pH to change from the acidic stomach to the alkaline duodenum, as well as a change in pressure patterns prior to emptying. Gastroparesis was defined by a gastric emptying time exceeding 5 hours [11]. Pressure patterns are characterized as mean peak amplitude and mean contractions per minute. Mean peak amplitude is the quotient of the sum of amplitudes divided by the number of contractions. The location of the capsule was confirmed by autopsy after the animals were sacrificed after 24 hours.

Bottom Line: Gastric pH values were statistically significantly different (P<0.003) in the animals over time and ranged from 1.15 to 9.94 [5.73 ± 0.47 (mean ± SD)] with an interquartile range of 0.11 to 2.07.The capsule pressure recordings ranged from 2 to 4 mmHg [2.6 ± 0.5 mmHg (mean ± SD)].There was no change in pressure patterns or sudden rise of pH >3 pH units during 24 hours.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology, University of Wurzburg, Wurzburg, Germany. rauch_s@klinik.uni-wuerzburg.de

ABSTRACT

Background: Evaluation of gastric pH and motility in a porcine model of acute lung injury using a novel, wireless motility capsule.

Material/methods: A motility capsule was applied into the stomach of 7 Pietrain pigs with acute lung injury induced by high volume saline lavage. Wireless transmission of pH, pressure and temperature data was performed by a recorder attached to the animal's abdomen. Gastric motility was evaluated using pH and pressure values, and capsule location was confirmed by autopsy.

Results: Gastric pH values were statistically significantly different (P<0.003) in the animals over time and ranged from 1.15 to 9.94 [5.73 ± 0.47 (mean ± SD)] with an interquartile range of 0.11 to 2.07. The capsule pressure recordings ranged from 2 to 4 mmHg [2.6 ± 0.5 mmHg (mean ± SD)]. There was no change in pressure patterns or sudden rise of pH >3 pH units during 24 hours. All animals had a gastroparesis with the capsules located in the stomach as indicated by the pressure and pH data and confirmed by necropsy.

Conclusions: The preliminary data show that Pietrain pigs with acute lung injury have a high variability in gastric pH and severely disturbed gastric motility.

Show MeSH
Related in: MedlinePlus