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High-frequency electrical stimulation in the nucleus accumbens of morphine-treated rats suppresses neuronal firing in reward-related brain regions.

Hu WH, Bi YF, Zhang K, Meng FG, Zhang JG - Med. Sci. Monit. (2011)

Bottom Line: Conditioned place preference (CPP) behavior of the rats was evaluated to confirm morphine preference after morphine injection and CPP training for 10 days.The results suggest that electrical stimulation in the NAc can suppress neuronal firing in reward-related brain regions.The core and shell of the NAc play different roles in suppressing NAc neuronal firing as 2 stimulating targets.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

ABSTRACT

Background: Previous studies have reported that high-frequency stimulation (HFS) in the nucleus accumbens (NAc) is a potential treatment modality for drug craving and relapse. We aimed to explore the electrophysiological changes in reward-related brain regions during NAc stimulation and reveal the effects of stimulation frequency and target changes on NAc neuronal activities.

Material/methods: Twenty-eight rats were randomized into saline (n=8) and morphine (n=20) groups. The morphine group was further divided into core (n=10, only the core of the NAc was stimulated) and shell (n=10, only the shell of the NAc was stimulated) subgroups. Conditioned place preference (CPP) behavior of the rats was evaluated to confirm morphine preference after morphine injection and CPP training for 10 days. We recorded NAc neuronal responses to NAc core stimulation at different frequencies, as well as changes in VP and VTA neuronal firing during NAc core stimulation, and changes in NAc neuronal firing during NAc shell stimulation.

Results: The results indicate that high frequency stimulation was more effective in suppressing NAc neuronal activities than low frequency stimulation and that core stimulation was more effective than shell stimulation. Most VP neurons were inhibited by NAc core stimulation, while VTA neurons were not.

Conclusions: The results suggest that electrical stimulation in the NAc can suppress neuronal firing in reward-related brain regions. The stimulation might be frequency- dependent in suppressing neuronal firing. The core and shell of the NAc play different roles in suppressing NAc neuronal firing as 2 stimulating targets.

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Related in: MedlinePlus

Stimulation of the NAc shell at 130 Hz suppressed NAc neuronal firing. (A). Firing rates of NAc neurons (n=34) before and during NAc shell stimulation at 130Hz. Data was mean ±SEM, * stood for P<0.05, Paired-Samples t test. (B). Comparison of suppression rates of NAc neuronal firing between NAc core (n=27) and shell (n=34) stimulation at 130 Hz. Data was mean ±SEM, * stood for P<0.05, Independent-Sample t test.
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f6-medscimonit-17-6-br153: Stimulation of the NAc shell at 130 Hz suppressed NAc neuronal firing. (A). Firing rates of NAc neurons (n=34) before and during NAc shell stimulation at 130Hz. Data was mean ±SEM, * stood for P<0.05, Paired-Samples t test. (B). Comparison of suppression rates of NAc neuronal firing between NAc core (n=27) and shell (n=34) stimulation at 130 Hz. Data was mean ±SEM, * stood for P<0.05, Independent-Sample t test.

Mentions: We stimulated the NAc shell at 130Hz and recorded the changes in neuronal activities in the NAc. Shell HFS also showed an inhibitory effect on NAc neurons (Paired-Samples t test: t(33)=3.89, P<0.0001) (Figure 6A). Compared with core stimulation, the neurons recorded during shell stimulation were closer to the stimulating site [0.72±0.04 mm (shell) vs. 0.96±0.30 mm (core)]. However, the former showed a higher suppression rate than the latter, supporting the idea that core stimulation had a more powerful inhibitory effect (Independent-Sample t test: t(59)=2.59, P=0.01) (Figure 6B). Figure 7 showed the sites of stimulating electrode in the core or shell of the NAc and coordinates of the tested NAc neurons.


High-frequency electrical stimulation in the nucleus accumbens of morphine-treated rats suppresses neuronal firing in reward-related brain regions.

Hu WH, Bi YF, Zhang K, Meng FG, Zhang JG - Med. Sci. Monit. (2011)

Stimulation of the NAc shell at 130 Hz suppressed NAc neuronal firing. (A). Firing rates of NAc neurons (n=34) before and during NAc shell stimulation at 130Hz. Data was mean ±SEM, * stood for P<0.05, Paired-Samples t test. (B). Comparison of suppression rates of NAc neuronal firing between NAc core (n=27) and shell (n=34) stimulation at 130 Hz. Data was mean ±SEM, * stood for P<0.05, Independent-Sample t test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539543&req=5

f6-medscimonit-17-6-br153: Stimulation of the NAc shell at 130 Hz suppressed NAc neuronal firing. (A). Firing rates of NAc neurons (n=34) before and during NAc shell stimulation at 130Hz. Data was mean ±SEM, * stood for P<0.05, Paired-Samples t test. (B). Comparison of suppression rates of NAc neuronal firing between NAc core (n=27) and shell (n=34) stimulation at 130 Hz. Data was mean ±SEM, * stood for P<0.05, Independent-Sample t test.
Mentions: We stimulated the NAc shell at 130Hz and recorded the changes in neuronal activities in the NAc. Shell HFS also showed an inhibitory effect on NAc neurons (Paired-Samples t test: t(33)=3.89, P<0.0001) (Figure 6A). Compared with core stimulation, the neurons recorded during shell stimulation were closer to the stimulating site [0.72±0.04 mm (shell) vs. 0.96±0.30 mm (core)]. However, the former showed a higher suppression rate than the latter, supporting the idea that core stimulation had a more powerful inhibitory effect (Independent-Sample t test: t(59)=2.59, P=0.01) (Figure 6B). Figure 7 showed the sites of stimulating electrode in the core or shell of the NAc and coordinates of the tested NAc neurons.

Bottom Line: Conditioned place preference (CPP) behavior of the rats was evaluated to confirm morphine preference after morphine injection and CPP training for 10 days.The results suggest that electrical stimulation in the NAc can suppress neuronal firing in reward-related brain regions.The core and shell of the NAc play different roles in suppressing NAc neuronal firing as 2 stimulating targets.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

ABSTRACT

Background: Previous studies have reported that high-frequency stimulation (HFS) in the nucleus accumbens (NAc) is a potential treatment modality for drug craving and relapse. We aimed to explore the electrophysiological changes in reward-related brain regions during NAc stimulation and reveal the effects of stimulation frequency and target changes on NAc neuronal activities.

Material/methods: Twenty-eight rats were randomized into saline (n=8) and morphine (n=20) groups. The morphine group was further divided into core (n=10, only the core of the NAc was stimulated) and shell (n=10, only the shell of the NAc was stimulated) subgroups. Conditioned place preference (CPP) behavior of the rats was evaluated to confirm morphine preference after morphine injection and CPP training for 10 days. We recorded NAc neuronal responses to NAc core stimulation at different frequencies, as well as changes in VP and VTA neuronal firing during NAc core stimulation, and changes in NAc neuronal firing during NAc shell stimulation.

Results: The results indicate that high frequency stimulation was more effective in suppressing NAc neuronal activities than low frequency stimulation and that core stimulation was more effective than shell stimulation. Most VP neurons were inhibited by NAc core stimulation, while VTA neurons were not.

Conclusions: The results suggest that electrical stimulation in the NAc can suppress neuronal firing in reward-related brain regions. The stimulation might be frequency- dependent in suppressing neuronal firing. The core and shell of the NAc play different roles in suppressing NAc neuronal firing as 2 stimulating targets.

Show MeSH
Related in: MedlinePlus