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Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer.

Roszkowski K, Jozwicki W, Blaszczyk P, Mucha-Malecka A, Siomek A - Med. Sci. Monit. (2011)

Bottom Line: For the whole patient population (n=222) the median values of 8-oxoGua and 8-oxodG in urine samples were 12.44 (interquartile range: 8.14-20.33) [nmol/24 hr] and 6.05 (3.12-15.38) [nmol/24 hr], respectively.The median values of 8-oxoGua and 8-oxodG in urine samples of the control group (n=85) were 7.7 (4.65-10.15) [nmol/24 hr] and 2.2 (1.7-2.8) [nmol/24 hr], respectively.An assay that enables the determination of levels of basic markers of oxidative stress might be applied in clinical practice as an additional, helpful marker to diagnose cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy, Center of Oncology, Bydgoszcz, Poland. roszkowskik@co.bydgoszcz.pl

ABSTRACT

Background: The broad spectrum of oxidative damage DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2'-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidative damage DNA in leukocytes, was analyzed in cancer patients and healthy subjects.

Material/methods: 222 cancer patients and 134 healthy volunteers were included in the analysis, using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC.

Results: For the whole patient population (n=222) the median values of 8-oxoGua and 8-oxodG in urine samples were 12.44 (interquartile range: 8.14-20.33) [nmol/24 hr] and 6.05 (3.12-15.38) [nmol/24 hr], respectively. The median values of 8-oxoGua and 8-oxodG in urine samples of the control group (n=85) were 7.7 (4.65-10.15) [nmol/24 hr] and 2.2 (1.7-2.8) [nmol/24 hr], respectively. The level of 8-oxodG in DNA isolated from leukocytes of the patient population (n=179) and of the control group (n=134) was 4.93 (3.46-9.27) per 10'6 dG and 4.46 (3.82-5.31) per 10'6 dG, respectively.

Conclusions: The results suggest that oxidative stress in cancer patients, demonstrated by augmented amounts of these modifications in urine, could be typical not only for affected tissue but also for other tissues and even the whole organism. An assay that enables the determination of levels of basic markers of oxidative stress might be applied in clinical practice as an additional, helpful marker to diagnose cancer.

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Related in: MedlinePlus

Levels of 8-oxodG in DNA isolated from venous blood leucocytes in control group of healthy subjects and cancer patients before treatment (median values). Mann-Whitney Test p=0.0017.
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f3-medscimonit-17-6-cr329: Levels of 8-oxodG in DNA isolated from venous blood leucocytes in control group of healthy subjects and cancer patients before treatment (median values). Mann-Whitney Test p=0.0017.

Mentions: The results presented in the present study comparing amounts of 8-oxoGua and 8-oxodG excreted in urine daily as well as amounts of 8-oxodG in venous blood DNA leucocytes in patients with diagnosed cancer when compared with healthy subjects demonstrate higher levels of these modifications in cancer patients (Figures 1–3). Significantly elevated levels of analyzed markers of oxidative damage DNA may reflect the oxidative stress situation which accompanies cancer [34,35].


Oxidative damage DNA: 8-oxoGua and 8-oxodG as molecular markers of cancer.

Roszkowski K, Jozwicki W, Blaszczyk P, Mucha-Malecka A, Siomek A - Med. Sci. Monit. (2011)

Levels of 8-oxodG in DNA isolated from venous blood leucocytes in control group of healthy subjects and cancer patients before treatment (median values). Mann-Whitney Test p=0.0017.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539537&req=5

f3-medscimonit-17-6-cr329: Levels of 8-oxodG in DNA isolated from venous blood leucocytes in control group of healthy subjects and cancer patients before treatment (median values). Mann-Whitney Test p=0.0017.
Mentions: The results presented in the present study comparing amounts of 8-oxoGua and 8-oxodG excreted in urine daily as well as amounts of 8-oxodG in venous blood DNA leucocytes in patients with diagnosed cancer when compared with healthy subjects demonstrate higher levels of these modifications in cancer patients (Figures 1–3). Significantly elevated levels of analyzed markers of oxidative damage DNA may reflect the oxidative stress situation which accompanies cancer [34,35].

Bottom Line: For the whole patient population (n=222) the median values of 8-oxoGua and 8-oxodG in urine samples were 12.44 (interquartile range: 8.14-20.33) [nmol/24 hr] and 6.05 (3.12-15.38) [nmol/24 hr], respectively.The median values of 8-oxoGua and 8-oxodG in urine samples of the control group (n=85) were 7.7 (4.65-10.15) [nmol/24 hr] and 2.2 (1.7-2.8) [nmol/24 hr], respectively.An assay that enables the determination of levels of basic markers of oxidative stress might be applied in clinical practice as an additional, helpful marker to diagnose cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiotherapy, Center of Oncology, Bydgoszcz, Poland. roszkowskik@co.bydgoszcz.pl

ABSTRACT

Background: The broad spectrum of oxidative damage DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2'-deoxyguanosine), 8-oxoGua (8-oxo-7,8-dihydroguanine) as well as the level of oxidative damage DNA in leukocytes, was analyzed in cancer patients and healthy subjects.

Material/methods: 222 cancer patients and 134 healthy volunteers were included in the analysis, using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC.

Results: For the whole patient population (n=222) the median values of 8-oxoGua and 8-oxodG in urine samples were 12.44 (interquartile range: 8.14-20.33) [nmol/24 hr] and 6.05 (3.12-15.38) [nmol/24 hr], respectively. The median values of 8-oxoGua and 8-oxodG in urine samples of the control group (n=85) were 7.7 (4.65-10.15) [nmol/24 hr] and 2.2 (1.7-2.8) [nmol/24 hr], respectively. The level of 8-oxodG in DNA isolated from leukocytes of the patient population (n=179) and of the control group (n=134) was 4.93 (3.46-9.27) per 10'6 dG and 4.46 (3.82-5.31) per 10'6 dG, respectively.

Conclusions: The results suggest that oxidative stress in cancer patients, demonstrated by augmented amounts of these modifications in urine, could be typical not only for affected tissue but also for other tissues and even the whole organism. An assay that enables the determination of levels of basic markers of oxidative stress might be applied in clinical practice as an additional, helpful marker to diagnose cancer.

Show MeSH
Related in: MedlinePlus