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An animal model of femoral head osteonecrosis induced by a single injection of absolute alcohol: an experimental study.

Zhu ZH, Gao YS, Luo SH, Zeng BF, Zhang CQ - Med. Sci. Monit. (2011)

Bottom Line: Macrostructural and radiographic examinations revealed that the contour of both femoral heads was deformed slightly at 12 weeks, but a contour deformation with joint space narrowing was observed at 25 weeks.Histologically, a strong concordance with the natural history of ONFH in humans was found.The present model demonstrated bone trabeculae, marrow necrosis, a reconstruction deficiency and destruction of the microcirculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT

Background: The lack of an experimental animal model that can reliably mimic all stages of osteonecrosis of the femoral head has hindered progress toward the successful prevention and treatment of the disease.

Material/methods: A goat model of osteonecrosis of the femoral head (ONFH) was established and observed from the early to the intermediate-to-late stage of mechanical failure. Absolute alcohol was injected slowly into the center of bilateral femoral heads in 12 adult Small Tail Han goats. Postoperatively, the femoral heads were harvested and examined using macrostructural and histological analyses and radiographic and MRI examinations at weeks 4, 8, 12, and 25.

Results: Macrostructural and radiographic examinations revealed that the contour of both femoral heads was deformed slightly at 12 weeks, but a contour deformation with joint space narrowing was observed at 25 weeks. Histologically, a strong concordance with the natural history of ONFH in humans was found. The present model demonstrated bone trabeculae, marrow necrosis, a reconstruction deficiency and destruction of the microcirculation.

Conclusions: Among quadrupedal models, the goat model of ONFH, which is induced by a single injection of absolute alcohol, may be suitable and valuable for the evaluation of various therapeutics and side effects in the treatment of ONFH.

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(A) Histological specimen showed damaged trabecular resorption at week 4 (HE, 40×). (B) A number of the osteocyte-filled lacunae were replaced by empty lacunae at week 8 (HE, 100×). (C) Fibrous tissue with some new bone formed at week 12 (HE, 100×). (D) Massive bone fragmentation was showed in the marrow cavities at week 25 (HE, 100×). (E) Deformation of joint contour and irregularity of the subchondral bone and loss of chondrocytes was showed at week 25 (HE, 20×).
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f4-medscimonit-17-4-br97: (A) Histological specimen showed damaged trabecular resorption at week 4 (HE, 40×). (B) A number of the osteocyte-filled lacunae were replaced by empty lacunae at week 8 (HE, 100×). (C) Fibrous tissue with some new bone formed at week 12 (HE, 100×). (D) Massive bone fragmentation was showed in the marrow cavities at week 25 (HE, 100×). (E) Deformation of joint contour and irregularity of the subchondral bone and loss of chondrocytes was showed at week 25 (HE, 20×).

Mentions: The percentage of empty lacunae had decreased significantly (>35%). Massive amounts of trabecular bone had been absorbed. A large amount of bone debris and osteoclasts were detected in most of the marrow cavities, which were filled with dense fibrous tissues that exhibited a high level of cellularity with numerous fibrocytes. In the necrotic trabecular areas, active osteoblasts, bone debris and newly woven bone were present. Tenuous trabecular bone and microfractures were observed in the subchondral bone. The number of chondrocytes had decreased severely, and these cells were almost absent in the collapsed area (Figure 4A–E).


An animal model of femoral head osteonecrosis induced by a single injection of absolute alcohol: an experimental study.

Zhu ZH, Gao YS, Luo SH, Zeng BF, Zhang CQ - Med. Sci. Monit. (2011)

(A) Histological specimen showed damaged trabecular resorption at week 4 (HE, 40×). (B) A number of the osteocyte-filled lacunae were replaced by empty lacunae at week 8 (HE, 100×). (C) Fibrous tissue with some new bone formed at week 12 (HE, 100×). (D) Massive bone fragmentation was showed in the marrow cavities at week 25 (HE, 100×). (E) Deformation of joint contour and irregularity of the subchondral bone and loss of chondrocytes was showed at week 25 (HE, 20×).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539532&req=5

f4-medscimonit-17-4-br97: (A) Histological specimen showed damaged trabecular resorption at week 4 (HE, 40×). (B) A number of the osteocyte-filled lacunae were replaced by empty lacunae at week 8 (HE, 100×). (C) Fibrous tissue with some new bone formed at week 12 (HE, 100×). (D) Massive bone fragmentation was showed in the marrow cavities at week 25 (HE, 100×). (E) Deformation of joint contour and irregularity of the subchondral bone and loss of chondrocytes was showed at week 25 (HE, 20×).
Mentions: The percentage of empty lacunae had decreased significantly (>35%). Massive amounts of trabecular bone had been absorbed. A large amount of bone debris and osteoclasts were detected in most of the marrow cavities, which were filled with dense fibrous tissues that exhibited a high level of cellularity with numerous fibrocytes. In the necrotic trabecular areas, active osteoblasts, bone debris and newly woven bone were present. Tenuous trabecular bone and microfractures were observed in the subchondral bone. The number of chondrocytes had decreased severely, and these cells were almost absent in the collapsed area (Figure 4A–E).

Bottom Line: Macrostructural and radiographic examinations revealed that the contour of both femoral heads was deformed slightly at 12 weeks, but a contour deformation with joint space narrowing was observed at 25 weeks.Histologically, a strong concordance with the natural history of ONFH in humans was found.The present model demonstrated bone trabeculae, marrow necrosis, a reconstruction deficiency and destruction of the microcirculation.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedic Surgery, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.

ABSTRACT

Background: The lack of an experimental animal model that can reliably mimic all stages of osteonecrosis of the femoral head has hindered progress toward the successful prevention and treatment of the disease.

Material/methods: A goat model of osteonecrosis of the femoral head (ONFH) was established and observed from the early to the intermediate-to-late stage of mechanical failure. Absolute alcohol was injected slowly into the center of bilateral femoral heads in 12 adult Small Tail Han goats. Postoperatively, the femoral heads were harvested and examined using macrostructural and histological analyses and radiographic and MRI examinations at weeks 4, 8, 12, and 25.

Results: Macrostructural and radiographic examinations revealed that the contour of both femoral heads was deformed slightly at 12 weeks, but a contour deformation with joint space narrowing was observed at 25 weeks. Histologically, a strong concordance with the natural history of ONFH in humans was found. The present model demonstrated bone trabeculae, marrow necrosis, a reconstruction deficiency and destruction of the microcirculation.

Conclusions: Among quadrupedal models, the goat model of ONFH, which is induced by a single injection of absolute alcohol, may be suitable and valuable for the evaluation of various therapeutics and side effects in the treatment of ONFH.

Show MeSH
Related in: MedlinePlus