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Protective effect of bilberry (Vaccinium myrtillus) against doxorubicin-induced oxidative cardiotoxicity in rats.

Ashour OM, Elberry AA, Alahdal A, Al Mohamadi AM, Nagy AA, Abdel-Naim AB, Abdel-Sattar EA, Mohamadin AM - Med. Sci. Monit. (2011)

Bottom Line: Supplementations with bilberries were effective in reducing oxidative stress in many tissue injuries due their high content of antioxidants.Bilberry extract significantly inhibited DOX-provoked reduced glutathione depletion and accumulation of oxidized glutathione, malondialdehyde and protein carbonyls in cardiac tissues.This was accompanied by significant amelioration of reduced cardiac catalase, superoxide dismutase, and glutathione peroxidase activities; and increased cardiac myeloperoxidase activity in response to DOX challenge.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

ABSTRACT

Background: Doxorubicin (DOX) is a commonly used chemotherapeutic agent. It is associated with serious dose-limiting cardiotoxicity, which is at least partly caused by generation of reactive oxygen species (ROS). Supplementations with bilberries were effective in reducing oxidative stress in many tissue injuries due their high content of antioxidants. The present study investigated the potential protective effect of bilberry extract against DOX-induced cardiotoxicity in rats.

Material/methods: Rats were treated orally with a methanolic extract of bilberry for 10 days. DOX was injected intraperitoneally on day 7. Twenty-four hours after the last bilberry administration, rats were subjected to ECG study. Blood was then withdrawn and cardiac tissues were dissected for assessment of oxidative stress and cardiac tissue injury. Cardiac tissues were also subjected to histopathological examination.

Results: Bilberry extract significantly inhibited DOX-provoked reduced glutathione depletion and accumulation of oxidized glutathione, malondialdehyde and protein carbonyls in cardiac tissues. This was accompanied by significant amelioration of reduced cardiac catalase, superoxide dismutase, and glutathione peroxidase activities; and increased cardiac myeloperoxidase activity in response to DOX challenge. Pretreatment with bilberry significantly guarded against DOX-induced increase in serum activities of lactate dehydrogenase, creatine phosphokinase and creatine kinase-MB, as well as the level of troponin I. Bilberry alleviated ECG changes in rats treated with DOX and attenuated its pathological changes.

Conclusions: Bilberry protects against DOX-induced cardiotoxicity in rats. This can be attributed, at least in part, to its antioxidant activity.

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Related in: MedlinePlus

The effect of bilberry on doxorubicin (DOX)-induced changes in ECG tracing. Control ECG (A) shows control heart rate, ST segment and interval, and QT interval. Bilberry ECG tracing (B) shows control heart rate, ST segment and interval, and QT interval. DOX-treated group (C) shows bradycardia, depressed long ST segment, and long QT interval. DOX+bilberry group (D) shows nearly normalized heart rate, ST segment and QT interval.
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f1-medscimonit-17-4-br110: The effect of bilberry on doxorubicin (DOX)-induced changes in ECG tracing. Control ECG (A) shows control heart rate, ST segment and interval, and QT interval. Bilberry ECG tracing (B) shows control heart rate, ST segment and interval, and QT interval. DOX-treated group (C) shows bradycardia, depressed long ST segment, and long QT interval. DOX+bilberry group (D) shows nearly normalized heart rate, ST segment and QT interval.

Mentions: ECG tracing showed normal cardiac activity in all rats in the control and bilberry groups, with a mean heart rate of 366±12 and 360±15 beat/min, respectively. Rats in the DOX-only treated group showed bradycardia (254±14 beat/min), ST segment depression and prolongation of both ST and QT intervals. Such ECG abnormalities were clearly improved in the bilberry+DOX group, as evidenced by normalization of heart rate, ST segment, and both ST and QT intervals (Table 1 and Figure 1).


Protective effect of bilberry (Vaccinium myrtillus) against doxorubicin-induced oxidative cardiotoxicity in rats.

Ashour OM, Elberry AA, Alahdal A, Al Mohamadi AM, Nagy AA, Abdel-Naim AB, Abdel-Sattar EA, Mohamadin AM - Med. Sci. Monit. (2011)

The effect of bilberry on doxorubicin (DOX)-induced changes in ECG tracing. Control ECG (A) shows control heart rate, ST segment and interval, and QT interval. Bilberry ECG tracing (B) shows control heart rate, ST segment and interval, and QT interval. DOX-treated group (C) shows bradycardia, depressed long ST segment, and long QT interval. DOX+bilberry group (D) shows nearly normalized heart rate, ST segment and QT interval.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539517&req=5

f1-medscimonit-17-4-br110: The effect of bilberry on doxorubicin (DOX)-induced changes in ECG tracing. Control ECG (A) shows control heart rate, ST segment and interval, and QT interval. Bilberry ECG tracing (B) shows control heart rate, ST segment and interval, and QT interval. DOX-treated group (C) shows bradycardia, depressed long ST segment, and long QT interval. DOX+bilberry group (D) shows nearly normalized heart rate, ST segment and QT interval.
Mentions: ECG tracing showed normal cardiac activity in all rats in the control and bilberry groups, with a mean heart rate of 366±12 and 360±15 beat/min, respectively. Rats in the DOX-only treated group showed bradycardia (254±14 beat/min), ST segment depression and prolongation of both ST and QT intervals. Such ECG abnormalities were clearly improved in the bilberry+DOX group, as evidenced by normalization of heart rate, ST segment, and both ST and QT intervals (Table 1 and Figure 1).

Bottom Line: Supplementations with bilberries were effective in reducing oxidative stress in many tissue injuries due their high content of antioxidants.Bilberry extract significantly inhibited DOX-provoked reduced glutathione depletion and accumulation of oxidized glutathione, malondialdehyde and protein carbonyls in cardiac tissues.This was accompanied by significant amelioration of reduced cardiac catalase, superoxide dismutase, and glutathione peroxidase activities; and increased cardiac myeloperoxidase activity in response to DOX challenge.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.

ABSTRACT

Background: Doxorubicin (DOX) is a commonly used chemotherapeutic agent. It is associated with serious dose-limiting cardiotoxicity, which is at least partly caused by generation of reactive oxygen species (ROS). Supplementations with bilberries were effective in reducing oxidative stress in many tissue injuries due their high content of antioxidants. The present study investigated the potential protective effect of bilberry extract against DOX-induced cardiotoxicity in rats.

Material/methods: Rats were treated orally with a methanolic extract of bilberry for 10 days. DOX was injected intraperitoneally on day 7. Twenty-four hours after the last bilberry administration, rats were subjected to ECG study. Blood was then withdrawn and cardiac tissues were dissected for assessment of oxidative stress and cardiac tissue injury. Cardiac tissues were also subjected to histopathological examination.

Results: Bilberry extract significantly inhibited DOX-provoked reduced glutathione depletion and accumulation of oxidized glutathione, malondialdehyde and protein carbonyls in cardiac tissues. This was accompanied by significant amelioration of reduced cardiac catalase, superoxide dismutase, and glutathione peroxidase activities; and increased cardiac myeloperoxidase activity in response to DOX challenge. Pretreatment with bilberry significantly guarded against DOX-induced increase in serum activities of lactate dehydrogenase, creatine phosphokinase and creatine kinase-MB, as well as the level of troponin I. Bilberry alleviated ECG changes in rats treated with DOX and attenuated its pathological changes.

Conclusions: Bilberry protects against DOX-induced cardiotoxicity in rats. This can be attributed, at least in part, to its antioxidant activity.

Show MeSH
Related in: MedlinePlus