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Expression of Th-17 and RORγt mRNA in Behçet's Disease.

Hamzaoui K, Bouali E, Ghorbel I, Khanfir M, Houman H, Hamzaoui A - Med. Sci. Monit. (2011)

Bottom Line: The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients.RORγt determined Th17 cell might be involved with increased IL-17A in BD.Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

View Article: PubMed Central - PubMed

Affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Tunisia. kamel.hamzaoui@gmail.com

ABSTRACT

Background: To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD).

Material/methods: Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs).

Results: We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs.

Conclusions: RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

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Related in: MedlinePlus

Up-regulation of adhesion molecule mRNA expression in human umbilical vein endothelial cells (HUVECs) by interleukin-17A (IL-17A) derived from patients with active Behçet’s disease (BD). (A): PBMCs from patients with active BD (n=20), MS (n=12), SLE (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay. (B): Quantitative reverse transcription-polymerase chain reaction was performed to determine the expression of adhesion molecule mRNA in HUVECs induced by supernatants from active BD patients from the culture of peripheral blood mononuclear cells (PBMCs) derived from patients with active BD (n=10).
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f5-medscimonit-17-4-cr227: Up-regulation of adhesion molecule mRNA expression in human umbilical vein endothelial cells (HUVECs) by interleukin-17A (IL-17A) derived from patients with active Behçet’s disease (BD). (A): PBMCs from patients with active BD (n=20), MS (n=12), SLE (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay. (B): Quantitative reverse transcription-polymerase chain reaction was performed to determine the expression of adhesion molecule mRNA in HUVECs induced by supernatants from active BD patients from the culture of peripheral blood mononuclear cells (PBMCs) derived from patients with active BD (n=10).

Mentions: PBMCs from patients with active BD (n=40), with MS patients (n=12) and with SLE patients (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay (Figure 5A). IL-17A secretion from stimulated PBMC from patients with active BD was increased (1092±270 pg/ml) compared with that in samples from healthy controls (580±178 pg/ml; P=0.0001). No significant difference was observed between active BD and MS patients (1130±270 pg/ml; P=0.685). However low significant difference was observed when active BD were compared to SLE patients (1500±310 pg/ml; P=0.0048).


Expression of Th-17 and RORγt mRNA in Behçet's Disease.

Hamzaoui K, Bouali E, Ghorbel I, Khanfir M, Houman H, Hamzaoui A - Med. Sci. Monit. (2011)

Up-regulation of adhesion molecule mRNA expression in human umbilical vein endothelial cells (HUVECs) by interleukin-17A (IL-17A) derived from patients with active Behçet’s disease (BD). (A): PBMCs from patients with active BD (n=20), MS (n=12), SLE (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay. (B): Quantitative reverse transcription-polymerase chain reaction was performed to determine the expression of adhesion molecule mRNA in HUVECs induced by supernatants from active BD patients from the culture of peripheral blood mononuclear cells (PBMCs) derived from patients with active BD (n=10).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539514&req=5

f5-medscimonit-17-4-cr227: Up-regulation of adhesion molecule mRNA expression in human umbilical vein endothelial cells (HUVECs) by interleukin-17A (IL-17A) derived from patients with active Behçet’s disease (BD). (A): PBMCs from patients with active BD (n=20), MS (n=12), SLE (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay. (B): Quantitative reverse transcription-polymerase chain reaction was performed to determine the expression of adhesion molecule mRNA in HUVECs induced by supernatants from active BD patients from the culture of peripheral blood mononuclear cells (PBMCs) derived from patients with active BD (n=10).
Mentions: PBMCs from patients with active BD (n=40), with MS patients (n=12) and with SLE patients (n=20) and control subjects (n=12) were stimulated for 5 hours with phorbol myristate acetate and ionomycin, and supernatants were collected to detect IL-17A by enzyme-linked immunosorbent assay (Figure 5A). IL-17A secretion from stimulated PBMC from patients with active BD was increased (1092±270 pg/ml) compared with that in samples from healthy controls (580±178 pg/ml; P=0.0001). No significant difference was observed between active BD and MS patients (1130±270 pg/ml; P=0.685). However low significant difference was observed when active BD were compared to SLE patients (1500±310 pg/ml; P=0.0048).

Bottom Line: The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients.RORγt determined Th17 cell might be involved with increased IL-17A in BD.Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

View Article: PubMed Central - PubMed

Affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Tunisia. kamel.hamzaoui@gmail.com

ABSTRACT

Background: To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD).

Material/methods: Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs).

Results: We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs.

Conclusions: RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

Show MeSH
Related in: MedlinePlus