Limits...
Expression of Th-17 and RORγt mRNA in Behçet's Disease.

Hamzaoui K, Bouali E, Ghorbel I, Khanfir M, Houman H, Hamzaoui A - Med. Sci. Monit. (2011)

Bottom Line: The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients.RORγt determined Th17 cell might be involved with increased IL-17A in BD.Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

View Article: PubMed Central - PubMed

Affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Tunisia. kamel.hamzaoui@gmail.com

ABSTRACT

Background: To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD).

Material/methods: Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs).

Results: We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs.

Conclusions: RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

Show MeSH

Related in: MedlinePlus

Increased circulating Th17 frequency is correlated with Behçet’s diseases (BD) activity. (A): Representative figures of Th17 cells from a normal control and one active BD patient. CD4+IL-17+ Th17 were gated from CD3+ T cells. (B): Percentage of Th17/CD4+ cells from 45 active BD patients, 28 remission BD patients, 40 healthy controls (HC), 12 multiple sclerosis (MS) and 20 systemic lupus erythematosus (SLE) patients. The frequencies of Th17 cells in active BD patients and all two patient groups are significantly higher than for normal controls and remission BD patients. The medians are indicated by a line inside each box, the 25th and 75th percentiles by the box limits, the lower and upper error bars represent the 10th and 90th percentiles. (C1): Histologic feature of middle dermis of erythema nodosum (EN)-like eruption taken from active BD patient. Mononuclear cells are mainly infiltrated around and in the walls of vessels in the middle dermis (HE, 200×) [30]. Panels on the right show immunochemical histology of the EN-like eruption with a predominance of CD3+ lymphocytes around vessels. (C2): Lesional skin was further stained with IL-17. Bars =100 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539514&req=5

f3-medscimonit-17-4-cr227: Increased circulating Th17 frequency is correlated with Behçet’s diseases (BD) activity. (A): Representative figures of Th17 cells from a normal control and one active BD patient. CD4+IL-17+ Th17 were gated from CD3+ T cells. (B): Percentage of Th17/CD4+ cells from 45 active BD patients, 28 remission BD patients, 40 healthy controls (HC), 12 multiple sclerosis (MS) and 20 systemic lupus erythematosus (SLE) patients. The frequencies of Th17 cells in active BD patients and all two patient groups are significantly higher than for normal controls and remission BD patients. The medians are indicated by a line inside each box, the 25th and 75th percentiles by the box limits, the lower and upper error bars represent the 10th and 90th percentiles. (C1): Histologic feature of middle dermis of erythema nodosum (EN)-like eruption taken from active BD patient. Mononuclear cells are mainly infiltrated around and in the walls of vessels in the middle dermis (HE, 200×) [30]. Panels on the right show immunochemical histology of the EN-like eruption with a predominance of CD3+ lymphocytes around vessels. (C2): Lesional skin was further stained with IL-17. Bars =100 μm.

Mentions: To determine whether Th17 cells are present in BD (45 patients with active BD and 28 patients in remission BD), PBMC were isolated and stimulated with PMA and ionomycin in the presence of brefeldin A (Figure 3A, B). We compared the Th17 cell proportions in BD, in healthy controls and in control diseases (MS and SLE patients). There was a significantly higher frequency of circulating Th17 cells in active BD patients (2.87±1.2%) compared to healthy controls (0.64±0.37%; P=0.0001) and remission BD patients (0.75±0.37%; P=0.0001). No Significant differences were observed between active BD patients, MS patients (2.96±1.21%; P=0.52) and SLE patients (2.75±1.31; P=0.47). No difference was also observed between MS and SLE patients (P=0.663).


Expression of Th-17 and RORγt mRNA in Behçet's Disease.

Hamzaoui K, Bouali E, Ghorbel I, Khanfir M, Houman H, Hamzaoui A - Med. Sci. Monit. (2011)

Increased circulating Th17 frequency is correlated with Behçet’s diseases (BD) activity. (A): Representative figures of Th17 cells from a normal control and one active BD patient. CD4+IL-17+ Th17 were gated from CD3+ T cells. (B): Percentage of Th17/CD4+ cells from 45 active BD patients, 28 remission BD patients, 40 healthy controls (HC), 12 multiple sclerosis (MS) and 20 systemic lupus erythematosus (SLE) patients. The frequencies of Th17 cells in active BD patients and all two patient groups are significantly higher than for normal controls and remission BD patients. The medians are indicated by a line inside each box, the 25th and 75th percentiles by the box limits, the lower and upper error bars represent the 10th and 90th percentiles. (C1): Histologic feature of middle dermis of erythema nodosum (EN)-like eruption taken from active BD patient. Mononuclear cells are mainly infiltrated around and in the walls of vessels in the middle dermis (HE, 200×) [30]. Panels on the right show immunochemical histology of the EN-like eruption with a predominance of CD3+ lymphocytes around vessels. (C2): Lesional skin was further stained with IL-17. Bars =100 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539514&req=5

f3-medscimonit-17-4-cr227: Increased circulating Th17 frequency is correlated with Behçet’s diseases (BD) activity. (A): Representative figures of Th17 cells from a normal control and one active BD patient. CD4+IL-17+ Th17 were gated from CD3+ T cells. (B): Percentage of Th17/CD4+ cells from 45 active BD patients, 28 remission BD patients, 40 healthy controls (HC), 12 multiple sclerosis (MS) and 20 systemic lupus erythematosus (SLE) patients. The frequencies of Th17 cells in active BD patients and all two patient groups are significantly higher than for normal controls and remission BD patients. The medians are indicated by a line inside each box, the 25th and 75th percentiles by the box limits, the lower and upper error bars represent the 10th and 90th percentiles. (C1): Histologic feature of middle dermis of erythema nodosum (EN)-like eruption taken from active BD patient. Mononuclear cells are mainly infiltrated around and in the walls of vessels in the middle dermis (HE, 200×) [30]. Panels on the right show immunochemical histology of the EN-like eruption with a predominance of CD3+ lymphocytes around vessels. (C2): Lesional skin was further stained with IL-17. Bars =100 μm.
Mentions: To determine whether Th17 cells are present in BD (45 patients with active BD and 28 patients in remission BD), PBMC were isolated and stimulated with PMA and ionomycin in the presence of brefeldin A (Figure 3A, B). We compared the Th17 cell proportions in BD, in healthy controls and in control diseases (MS and SLE patients). There was a significantly higher frequency of circulating Th17 cells in active BD patients (2.87±1.2%) compared to healthy controls (0.64±0.37%; P=0.0001) and remission BD patients (0.75±0.37%; P=0.0001). No Significant differences were observed between active BD patients, MS patients (2.96±1.21%; P=0.52) and SLE patients (2.75±1.31; P=0.47). No difference was also observed between MS and SLE patients (P=0.663).

Bottom Line: The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients.RORγt determined Th17 cell might be involved with increased IL-17A in BD.Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

View Article: PubMed Central - PubMed

Affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Tunisia. kamel.hamzaoui@gmail.com

ABSTRACT

Background: To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD).

Material/methods: Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs).

Results: We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs.

Conclusions: RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

Show MeSH
Related in: MedlinePlus