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Expression of Th-17 and RORγt mRNA in Behçet's Disease.

Hamzaoui K, Bouali E, Ghorbel I, Khanfir M, Houman H, Hamzaoui A - Med. Sci. Monit. (2011)

Bottom Line: The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients.RORγt determined Th17 cell might be involved with increased IL-17A in BD.Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

View Article: PubMed Central - PubMed

Affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Tunisia. kamel.hamzaoui@gmail.com

ABSTRACT

Background: To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD).

Material/methods: Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs).

Results: We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs.

Conclusions: RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

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Related in: MedlinePlus

Correlation between plasma IL-17 levels in 45 active BD patients and biological parameters CRP and ESR using Pearson’s correlation coefficient. Erythrocyte sedimentation rate (ESR: mm/h) and C-reactive protein (CRP: mg/L) expressed as median (range) values in active BD patients were significantly increased in active BD patients [ESR: 35.76 (2–90); CRP: 107.5 (3–260)] compared to healthy controls [ESR: 6 (2–17); CRP: 3.2 (3–9); P<0.0001]. (A): Positive correlation between plasma IL-17A level and CRP (r=0.687; P=0.0021) in active Behçet’s disease (BD). (B): Significant positive correlation was observed between plasma IL-17A level and ESR (r=0.597; P=0.003).
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f2-medscimonit-17-4-cr227: Correlation between plasma IL-17 levels in 45 active BD patients and biological parameters CRP and ESR using Pearson’s correlation coefficient. Erythrocyte sedimentation rate (ESR: mm/h) and C-reactive protein (CRP: mg/L) expressed as median (range) values in active BD patients were significantly increased in active BD patients [ESR: 35.76 (2–90); CRP: 107.5 (3–260)] compared to healthy controls [ESR: 6 (2–17); CRP: 3.2 (3–9); P<0.0001]. (A): Positive correlation between plasma IL-17A level and CRP (r=0.687; P=0.0021) in active Behçet’s disease (BD). (B): Significant positive correlation was observed between plasma IL-17A level and ESR (r=0.597; P=0.003).

Mentions: Twelve patients with BD were studied for IL-17 levels during active and remission stages (Figure 1B). These patients have oral ulcer, genital ulcer, ocular symptoms and arthritis. During their remission stage, they exhibited a decreased IL-17 level which contrasted with their IL-17A levels during the active stage: (active stage: 20.73±3.16 pg/ml; remission stage: 12.45±3.52 pg/ml; P=0.0001). None of these patients had vascular lesions, CNS involvement or pulmonary manifestation. This result indicates the role of IL-17 levels in the inflammatory process in BD as reported in other inflammatory diseases [28]. Significant correlation were observed between CRP (r=0.687; P=0.0021), ESR (r=0.597; P=0.003) and plasma IL-17 levels (Figure 2A, B).


Expression of Th-17 and RORγt mRNA in Behçet's Disease.

Hamzaoui K, Bouali E, Ghorbel I, Khanfir M, Houman H, Hamzaoui A - Med. Sci. Monit. (2011)

Correlation between plasma IL-17 levels in 45 active BD patients and biological parameters CRP and ESR using Pearson’s correlation coefficient. Erythrocyte sedimentation rate (ESR: mm/h) and C-reactive protein (CRP: mg/L) expressed as median (range) values in active BD patients were significantly increased in active BD patients [ESR: 35.76 (2–90); CRP: 107.5 (3–260)] compared to healthy controls [ESR: 6 (2–17); CRP: 3.2 (3–9); P<0.0001]. (A): Positive correlation between plasma IL-17A level and CRP (r=0.687; P=0.0021) in active Behçet’s disease (BD). (B): Significant positive correlation was observed between plasma IL-17A level and ESR (r=0.597; P=0.003).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539514&req=5

f2-medscimonit-17-4-cr227: Correlation between plasma IL-17 levels in 45 active BD patients and biological parameters CRP and ESR using Pearson’s correlation coefficient. Erythrocyte sedimentation rate (ESR: mm/h) and C-reactive protein (CRP: mg/L) expressed as median (range) values in active BD patients were significantly increased in active BD patients [ESR: 35.76 (2–90); CRP: 107.5 (3–260)] compared to healthy controls [ESR: 6 (2–17); CRP: 3.2 (3–9); P<0.0001]. (A): Positive correlation between plasma IL-17A level and CRP (r=0.687; P=0.0021) in active Behçet’s disease (BD). (B): Significant positive correlation was observed between plasma IL-17A level and ESR (r=0.597; P=0.003).
Mentions: Twelve patients with BD were studied for IL-17 levels during active and remission stages (Figure 1B). These patients have oral ulcer, genital ulcer, ocular symptoms and arthritis. During their remission stage, they exhibited a decreased IL-17 level which contrasted with their IL-17A levels during the active stage: (active stage: 20.73±3.16 pg/ml; remission stage: 12.45±3.52 pg/ml; P=0.0001). None of these patients had vascular lesions, CNS involvement or pulmonary manifestation. This result indicates the role of IL-17 levels in the inflammatory process in BD as reported in other inflammatory diseases [28]. Significant correlation were observed between CRP (r=0.687; P=0.0021), ESR (r=0.597; P=0.003) and plasma IL-17 levels (Figure 2A, B).

Bottom Line: The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients.RORγt determined Th17 cell might be involved with increased IL-17A in BD.Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

View Article: PubMed Central - PubMed

Affiliation: Tunis El Manar University, Medicine Faculty of Tunis, Tunisia. kamel.hamzaoui@gmail.com

ABSTRACT

Background: To investigate plasma IL-17 level and the expression of Th17 cell transcription factor RORγt in the pathogenesis of Behçet's Disease (BD).

Material/methods: Blood samples were collected from 73 patients with BD (45 patients were in active stage), 20 systemic lupus erythematosus (SLE) and 12 multiple sclerosis patients (MS). Twelve patients with BD were investigated both in their active and remission stages. Samples were processed to detect IL-17A level in plasma by enzyme-linked immunosorbent assay (ELISA). Related gene expression was assessed by real-time reverse transcription polymerase chain reaction. Function of Th17 cells in active BD patients with erythema nodosum (EN)-like eruption was studied in relation to human umbilical vein endothelial cells (HUVECs).

Results: We demonstrated the presence of Th17 cells and RORγt among the peripheral blood mononuclear cells (PBMC). The percentage of circulating Th17 cells and the ability to produce interleukin-17A (IL-17A) were increased in samples derived from patients with active BD, MS and SLE patients. We observed that IL-17A from patients with active BD could induce adhesion molecule messenger RNA expression in HUVECs.

Conclusions: RORγt determined Th17 cell might be involved with increased IL-17A in BD. Our results indicate that IL-17 contributes to the active proinflammatory pattern that is characteristic of inflammatory diseases and patients with active BD.

Show MeSH
Related in: MedlinePlus