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Effects of early administration of a novel anticholinergic drug on acute respiratory distress syndrome induced by sepsis.

Li H, Qian Z, Li J, Han X, Liu M - Med. Sci. Monit. (2011)

Bottom Line: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis.In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC.We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, China.

ABSTRACT

Background: Acute respiratory distress syndrome (ARDS) is the inflammatory disorder of the lung most commonly caused by sepsis. It was hypothesized that treating the lung with penehyclidine hydrochloride (PHC), a new type of hyoscyamus drug, early in the development of sepsis could diminish the lung dysfunction.

Material/methods: Sprague-Dawley rats were divided into 4 groups: 1) a control group; 2) a sham-operated group; 3) a cecal ligation and puncture (CLP) group; 4) a PHC-treated group. One hour after CLP surgery, rats were either untreated or treated with PHC via intraperitoneal injection. Lung wet/dry weight ratio, bronchoalveolar lavage fluid (BALF), serum tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), total nitrite/nitrate (NOx), superoxide dismutase (SOD), malondialdehyde (MDA) in lung tissues, and pulmonary functions were examined 24 hour after surgery. Another 60 rats were randomly assigned to 4 equal groups to observe survival status 96 hours after surgery.

Results: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis. In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC. In PHC-treated groups, the administered PHC had a significant protective effect on the lung dysfunction induced by sepsis.

Conclusions: We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

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Related in: MedlinePlus

Survival curve of four groups of rat over the observation period. Survival rate of non anesthetized non-operated controls and sham controls was 100% and 93.3%, respectively. Cecal ligation and puncture/saline rat corresponded to a mortality rate of 53.3%; CLP/PHC, 40.0%.
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f4-medscimonit-17-11-br319: Survival curve of four groups of rat over the observation period. Survival rate of non anesthetized non-operated controls and sham controls was 100% and 93.3%, respectively. Cecal ligation and puncture/saline rat corresponded to a mortality rate of 53.3%; CLP/PHC, 40.0%.

Mentions: After 4 days of observation, only 1 rat died from hemorrhea in the sham control group. In the CLP/saline group and CLP/PHC group, mortality rates were 53.3% (8/15) and 40% (6/15), respectively, and the difference was significant compared with sham controls (log rank=8.117, p=0.004; log rank=4.778, p=0.029) (Figure 4). At the same time, mortality was dramatically elevated in the CLP/saline group, while PHC that was administrated 1 h after surgery decreased the mortality rate (40.0% vs. 53.3%). However, there were no significant differences between the PHC-treated group and the CLP/saline group (log rank=0.722, p=0.395) (Figure 4).


Effects of early administration of a novel anticholinergic drug on acute respiratory distress syndrome induced by sepsis.

Li H, Qian Z, Li J, Han X, Liu M - Med. Sci. Monit. (2011)

Survival curve of four groups of rat over the observation period. Survival rate of non anesthetized non-operated controls and sham controls was 100% and 93.3%, respectively. Cecal ligation and puncture/saline rat corresponded to a mortality rate of 53.3%; CLP/PHC, 40.0%.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539499&req=5

f4-medscimonit-17-11-br319: Survival curve of four groups of rat over the observation period. Survival rate of non anesthetized non-operated controls and sham controls was 100% and 93.3%, respectively. Cecal ligation and puncture/saline rat corresponded to a mortality rate of 53.3%; CLP/PHC, 40.0%.
Mentions: After 4 days of observation, only 1 rat died from hemorrhea in the sham control group. In the CLP/saline group and CLP/PHC group, mortality rates were 53.3% (8/15) and 40% (6/15), respectively, and the difference was significant compared with sham controls (log rank=8.117, p=0.004; log rank=4.778, p=0.029) (Figure 4). At the same time, mortality was dramatically elevated in the CLP/saline group, while PHC that was administrated 1 h after surgery decreased the mortality rate (40.0% vs. 53.3%). However, there were no significant differences between the PHC-treated group and the CLP/saline group (log rank=0.722, p=0.395) (Figure 4).

Bottom Line: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis.In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC.We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, China.

ABSTRACT

Background: Acute respiratory distress syndrome (ARDS) is the inflammatory disorder of the lung most commonly caused by sepsis. It was hypothesized that treating the lung with penehyclidine hydrochloride (PHC), a new type of hyoscyamus drug, early in the development of sepsis could diminish the lung dysfunction.

Material/methods: Sprague-Dawley rats were divided into 4 groups: 1) a control group; 2) a sham-operated group; 3) a cecal ligation and puncture (CLP) group; 4) a PHC-treated group. One hour after CLP surgery, rats were either untreated or treated with PHC via intraperitoneal injection. Lung wet/dry weight ratio, bronchoalveolar lavage fluid (BALF), serum tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), total nitrite/nitrate (NOx), superoxide dismutase (SOD), malondialdehyde (MDA) in lung tissues, and pulmonary functions were examined 24 hour after surgery. Another 60 rats were randomly assigned to 4 equal groups to observe survival status 96 hours after surgery.

Results: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis. In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC. In PHC-treated groups, the administered PHC had a significant protective effect on the lung dysfunction induced by sepsis.

Conclusions: We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

Show MeSH
Related in: MedlinePlus