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Effects of early administration of a novel anticholinergic drug on acute respiratory distress syndrome induced by sepsis.

Li H, Qian Z, Li J, Han X, Liu M - Med. Sci. Monit. (2011)

Bottom Line: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis.In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC.We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, China.

ABSTRACT

Background: Acute respiratory distress syndrome (ARDS) is the inflammatory disorder of the lung most commonly caused by sepsis. It was hypothesized that treating the lung with penehyclidine hydrochloride (PHC), a new type of hyoscyamus drug, early in the development of sepsis could diminish the lung dysfunction.

Material/methods: Sprague-Dawley rats were divided into 4 groups: 1) a control group; 2) a sham-operated group; 3) a cecal ligation and puncture (CLP) group; 4) a PHC-treated group. One hour after CLP surgery, rats were either untreated or treated with PHC via intraperitoneal injection. Lung wet/dry weight ratio, bronchoalveolar lavage fluid (BALF), serum tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), total nitrite/nitrate (NOx), superoxide dismutase (SOD), malondialdehyde (MDA) in lung tissues, and pulmonary functions were examined 24 hour after surgery. Another 60 rats were randomly assigned to 4 equal groups to observe survival status 96 hours after surgery.

Results: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis. In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC. In PHC-treated groups, the administered PHC had a significant protective effect on the lung dysfunction induced by sepsis.

Conclusions: We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

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Related in: MedlinePlus

Concentrations of the inflammatory cytokines TNF-α (A), IL-6 (B), IL-10 (C) and NOx (D) in the Blood plasma from the 4 experimental groups outlined in MATERIALS AND METHODS. Values are mean ±S.D.; (Control n=15; Sham, n=15; CLP/saline, n=15 at 8 h, n=13 at 16 h, n=12 at 24 h; CLP/PHC, n=15 at 8 h, n=14 at 16 h, n=13 at 24 h; Compared with CLP/saline group, * p<0.001.
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f3-medscimonit-17-11-br319: Concentrations of the inflammatory cytokines TNF-α (A), IL-6 (B), IL-10 (C) and NOx (D) in the Blood plasma from the 4 experimental groups outlined in MATERIALS AND METHODS. Values are mean ±S.D.; (Control n=15; Sham, n=15; CLP/saline, n=15 at 8 h, n=13 at 16 h, n=12 at 24 h; CLP/PHC, n=15 at 8 h, n=14 at 16 h, n=13 at 24 h; Compared with CLP/saline group, * p<0.001.

Mentions: TNF-α and IL-6 levels were significantly elevated in the CLP/saline group when compared with Sham group controls at 3 time points (each p<0.001) (Figure 3). PHC treatment effectively inhibited this elevation in systemic cytokine levels. There was a significant difference in plasma levels of TNF-α (p<0.001) and IL-6 (p<0.001) between the CLP/saline group and the CLP/PHC group as mentioned above. IL-10 levels were significantly lower in CLP/saline rats compared with the CLP/PHC group at all defined time points (p<0.001).


Effects of early administration of a novel anticholinergic drug on acute respiratory distress syndrome induced by sepsis.

Li H, Qian Z, Li J, Han X, Liu M - Med. Sci. Monit. (2011)

Concentrations of the inflammatory cytokines TNF-α (A), IL-6 (B), IL-10 (C) and NOx (D) in the Blood plasma from the 4 experimental groups outlined in MATERIALS AND METHODS. Values are mean ±S.D.; (Control n=15; Sham, n=15; CLP/saline, n=15 at 8 h, n=13 at 16 h, n=12 at 24 h; CLP/PHC, n=15 at 8 h, n=14 at 16 h, n=13 at 24 h; Compared with CLP/saline group, * p<0.001.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539499&req=5

f3-medscimonit-17-11-br319: Concentrations of the inflammatory cytokines TNF-α (A), IL-6 (B), IL-10 (C) and NOx (D) in the Blood plasma from the 4 experimental groups outlined in MATERIALS AND METHODS. Values are mean ±S.D.; (Control n=15; Sham, n=15; CLP/saline, n=15 at 8 h, n=13 at 16 h, n=12 at 24 h; CLP/PHC, n=15 at 8 h, n=14 at 16 h, n=13 at 24 h; Compared with CLP/saline group, * p<0.001.
Mentions: TNF-α and IL-6 levels were significantly elevated in the CLP/saline group when compared with Sham group controls at 3 time points (each p<0.001) (Figure 3). PHC treatment effectively inhibited this elevation in systemic cytokine levels. There was a significant difference in plasma levels of TNF-α (p<0.001) and IL-6 (p<0.001) between the CLP/saline group and the CLP/PHC group as mentioned above. IL-10 levels were significantly lower in CLP/saline rats compared with the CLP/PHC group at all defined time points (p<0.001).

Bottom Line: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis.In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC.We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, China.

ABSTRACT

Background: Acute respiratory distress syndrome (ARDS) is the inflammatory disorder of the lung most commonly caused by sepsis. It was hypothesized that treating the lung with penehyclidine hydrochloride (PHC), a new type of hyoscyamus drug, early in the development of sepsis could diminish the lung dysfunction.

Material/methods: Sprague-Dawley rats were divided into 4 groups: 1) a control group; 2) a sham-operated group; 3) a cecal ligation and puncture (CLP) group; 4) a PHC-treated group. One hour after CLP surgery, rats were either untreated or treated with PHC via intraperitoneal injection. Lung wet/dry weight ratio, bronchoalveolar lavage fluid (BALF), serum tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), total nitrite/nitrate (NOx), superoxide dismutase (SOD), malondialdehyde (MDA) in lung tissues, and pulmonary functions were examined 24 hour after surgery. Another 60 rats were randomly assigned to 4 equal groups to observe survival status 96 hours after surgery.

Results: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis. In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC. In PHC-treated groups, the administered PHC had a significant protective effect on the lung dysfunction induced by sepsis.

Conclusions: We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

Show MeSH
Related in: MedlinePlus