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Effects of early administration of a novel anticholinergic drug on acute respiratory distress syndrome induced by sepsis.

Li H, Qian Z, Li J, Han X, Liu M - Med. Sci. Monit. (2011)

Bottom Line: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis.In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC.We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, China.

ABSTRACT

Background: Acute respiratory distress syndrome (ARDS) is the inflammatory disorder of the lung most commonly caused by sepsis. It was hypothesized that treating the lung with penehyclidine hydrochloride (PHC), a new type of hyoscyamus drug, early in the development of sepsis could diminish the lung dysfunction.

Material/methods: Sprague-Dawley rats were divided into 4 groups: 1) a control group; 2) a sham-operated group; 3) a cecal ligation and puncture (CLP) group; 4) a PHC-treated group. One hour after CLP surgery, rats were either untreated or treated with PHC via intraperitoneal injection. Lung wet/dry weight ratio, bronchoalveolar lavage fluid (BALF), serum tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), total nitrite/nitrate (NOx), superoxide dismutase (SOD), malondialdehyde (MDA) in lung tissues, and pulmonary functions were examined 24 hour after surgery. Another 60 rats were randomly assigned to 4 equal groups to observe survival status 96 hours after surgery.

Results: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis. In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC. In PHC-treated groups, the administered PHC had a significant protective effect on the lung dysfunction induced by sepsis.

Conclusions: We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

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Related in: MedlinePlus

The extent of acute lung injury evaluated by lung wet/dry weight ratio (A), and protein concentration in bronchoalveolar lavage (B). (Control n=15; Sham, n=15; CLP/saline, n=12; CLP/PHC, n=13; mean ± S.D.). Compared with CLP/saline group, * p<0.001.
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f2-medscimonit-17-11-br319: The extent of acute lung injury evaluated by lung wet/dry weight ratio (A), and protein concentration in bronchoalveolar lavage (B). (Control n=15; Sham, n=15; CLP/saline, n=12; CLP/PHC, n=13; mean ± S.D.). Compared with CLP/saline group, * p<0.001.

Mentions: Results of lung wet/dry weight ratio measurements are presented in Figure 2. Treatment with PHC produced a greater effect on ifying the sepsis-induced changes (Figure 2). Markedly elevated levels of wet/dry ratio and protein in BALF were lowered after administration of PHC (4.34±0.05 vs. 4.68±0.09; 4.60±0.26 vs. 7.36±0.48 mg/dl, each p<0.001). There was no significant difference in lung wet/dry weight ratio (p=0.168) and BALF (p=0.508) between the Control group and the Sham group.


Effects of early administration of a novel anticholinergic drug on acute respiratory distress syndrome induced by sepsis.

Li H, Qian Z, Li J, Han X, Liu M - Med. Sci. Monit. (2011)

The extent of acute lung injury evaluated by lung wet/dry weight ratio (A), and protein concentration in bronchoalveolar lavage (B). (Control n=15; Sham, n=15; CLP/saline, n=12; CLP/PHC, n=13; mean ± S.D.). Compared with CLP/saline group, * p<0.001.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539499&req=5

f2-medscimonit-17-11-br319: The extent of acute lung injury evaluated by lung wet/dry weight ratio (A), and protein concentration in bronchoalveolar lavage (B). (Control n=15; Sham, n=15; CLP/saline, n=12; CLP/PHC, n=13; mean ± S.D.). Compared with CLP/saline group, * p<0.001.
Mentions: Results of lung wet/dry weight ratio measurements are presented in Figure 2. Treatment with PHC produced a greater effect on ifying the sepsis-induced changes (Figure 2). Markedly elevated levels of wet/dry ratio and protein in BALF were lowered after administration of PHC (4.34±0.05 vs. 4.68±0.09; 4.60±0.26 vs. 7.36±0.48 mg/dl, each p<0.001). There was no significant difference in lung wet/dry weight ratio (p=0.168) and BALF (p=0.508) between the Control group and the Sham group.

Bottom Line: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis.In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC.We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, Hunan Provincial People's Hospital, Changsha, China.

ABSTRACT

Background: Acute respiratory distress syndrome (ARDS) is the inflammatory disorder of the lung most commonly caused by sepsis. It was hypothesized that treating the lung with penehyclidine hydrochloride (PHC), a new type of hyoscyamus drug, early in the development of sepsis could diminish the lung dysfunction.

Material/methods: Sprague-Dawley rats were divided into 4 groups: 1) a control group; 2) a sham-operated group; 3) a cecal ligation and puncture (CLP) group; 4) a PHC-treated group. One hour after CLP surgery, rats were either untreated or treated with PHC via intraperitoneal injection. Lung wet/dry weight ratio, bronchoalveolar lavage fluid (BALF), serum tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), total nitrite/nitrate (NOx), superoxide dismutase (SOD), malondialdehyde (MDA) in lung tissues, and pulmonary functions were examined 24 hour after surgery. Another 60 rats were randomly assigned to 4 equal groups to observe survival status 96 hours after surgery.

Results: Treatment of PHC markedly decreased TNF-α, IL-6, NOx, SOD, MDA content, protein concentration in BALF, and lung wet/dry weight ratio and enhanced SOD activity (p<0.05), which are indicative of PHC-induced suppression in the pathogenesis of ARDS caused by sepsis. In comparison to group CLP/saline, plasma IL-10 level markedly increased in group CLP/PHC. In PHC-treated groups, the administered PHC had a significant protective effect on the lung dysfunction induced by sepsis.

Conclusions: We conclude that administration of PHC at the time of a systemic insult can protect the lung from the damaging effects of sepsis.

Show MeSH
Related in: MedlinePlus