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Proanthocyanidin prevents myocardial ischemic injury in adult rats.

Guler A, Sahin MA, Yucel O, Yokusoglu M, Gamsizkan M, Ozal E, Demirkilic U, Arslan M - Med. Sci. Monit. (2011)

Bottom Line: MDA levels were significantly higher in IG and PCT than in CG and PCG.The activity of GPx was significantly lower in IG than in the other groups.The activities of CAT were significantly lower in IG and PCT than in the other groups and were significantly lower in IG than PCT.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Gulhane Military Medical School, Ankara, Turkey.

ABSTRACT

Background: Proanthocyanidin is a bioflavonoid known to have protective effect against oxidative injury. We investigated the cardioprotective effect of proanthocyanidin.

Material/methods: Thirty-two Rattus Norvegicus rats were categorized equally as the control group (CG), proanthocyanidin group (PCG), ischemia group (IG) and proanthocyanidin-treated group (PCT). Rats in CG and IG were fed standard rat food and PCG and PCT were fed standard rat food plus proanthocyanidin (100 mg/kg/day twice a day by oral gavage) for 3 weeks. In CG and PCG the myocardial samples were prepared immediately, and in IG and PCT hearts were placed in transport solution and kept at 4°C for 5 hours, then prepared for evaluation. Malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were measured.

Results: MDA levels were significantly higher in IG and PCT than in CG and PCG. The activity of SOD was significantly lower in IG and higher in PCG than in the other groups. The activity of GPx was significantly lower in IG than in the other groups. The activities of CAT were significantly lower in IG and PCT than in the other groups and were significantly lower in IG than PCT. Histopathologic evaluation revealed normal findings in CG and PCG. While ischemic injury was observed in IG, the content of muscle fibers was better preserved in PCT.

Conclusions: Proanthocyanidin may have a protective effect on myocardial ischemic injury.

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Related in: MedlinePlus

(A) Normal myocardial fibers in control group (He ×100). (B) Light vacuolar changes (arrow) in ischemia group (HE ×200). (C) Focal myocardial fiber degeneration in ischemia group (HE ×100). (D) Inflammatory cell infiltration (arrow) among the myocardial fibers in ischemia group (HE ×200). (E) Muscle fibers in treatment group are better preserved to compare to the ischemic ones (He ×100).
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f1-medscimonit-17-11-br326: (A) Normal myocardial fibers in control group (He ×100). (B) Light vacuolar changes (arrow) in ischemia group (HE ×200). (C) Focal myocardial fiber degeneration in ischemia group (HE ×100). (D) Inflammatory cell infiltration (arrow) among the myocardial fibers in ischemia group (HE ×200). (E) Muscle fibers in treatment group are better preserved to compare to the ischemic ones (He ×100).

Mentions: Myocardial fibrils were within normal range in CG and PCG (Figure 1A). In the IG, myofibrils were relatively indistinct in the muscle fibers that had picnotic-dark or pale nuclei and more acidophilic cytoplasm. Intracytoplasmic vacuoles were observed in some muscle fibers (Figure 1B). Muscle fiber organization was impaired and they were separated from each other due to intracellular edema in certain regions (Figure 1C). Inflammatory cell infiltrations were seen in some areas (Figure 1D). Degranulation of mast cells was rarely noted in the connective tissue. In PCT the organization of muscle fibers was better preserved compared to the IG; there were no vacuolar changes or necrosis (Figure 1E).


Proanthocyanidin prevents myocardial ischemic injury in adult rats.

Guler A, Sahin MA, Yucel O, Yokusoglu M, Gamsizkan M, Ozal E, Demirkilic U, Arslan M - Med. Sci. Monit. (2011)

(A) Normal myocardial fibers in control group (He ×100). (B) Light vacuolar changes (arrow) in ischemia group (HE ×200). (C) Focal myocardial fiber degeneration in ischemia group (HE ×100). (D) Inflammatory cell infiltration (arrow) among the myocardial fibers in ischemia group (HE ×200). (E) Muscle fibers in treatment group are better preserved to compare to the ischemic ones (He ×100).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539496&req=5

f1-medscimonit-17-11-br326: (A) Normal myocardial fibers in control group (He ×100). (B) Light vacuolar changes (arrow) in ischemia group (HE ×200). (C) Focal myocardial fiber degeneration in ischemia group (HE ×100). (D) Inflammatory cell infiltration (arrow) among the myocardial fibers in ischemia group (HE ×200). (E) Muscle fibers in treatment group are better preserved to compare to the ischemic ones (He ×100).
Mentions: Myocardial fibrils were within normal range in CG and PCG (Figure 1A). In the IG, myofibrils were relatively indistinct in the muscle fibers that had picnotic-dark or pale nuclei and more acidophilic cytoplasm. Intracytoplasmic vacuoles were observed in some muscle fibers (Figure 1B). Muscle fiber organization was impaired and they were separated from each other due to intracellular edema in certain regions (Figure 1C). Inflammatory cell infiltrations were seen in some areas (Figure 1D). Degranulation of mast cells was rarely noted in the connective tissue. In PCT the organization of muscle fibers was better preserved compared to the IG; there were no vacuolar changes or necrosis (Figure 1E).

Bottom Line: MDA levels were significantly higher in IG and PCT than in CG and PCG.The activity of GPx was significantly lower in IG than in the other groups.The activities of CAT were significantly lower in IG and PCT than in the other groups and were significantly lower in IG than PCT.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Gulhane Military Medical School, Ankara, Turkey.

ABSTRACT

Background: Proanthocyanidin is a bioflavonoid known to have protective effect against oxidative injury. We investigated the cardioprotective effect of proanthocyanidin.

Material/methods: Thirty-two Rattus Norvegicus rats were categorized equally as the control group (CG), proanthocyanidin group (PCG), ischemia group (IG) and proanthocyanidin-treated group (PCT). Rats in CG and IG were fed standard rat food and PCG and PCT were fed standard rat food plus proanthocyanidin (100 mg/kg/day twice a day by oral gavage) for 3 weeks. In CG and PCG the myocardial samples were prepared immediately, and in IG and PCT hearts were placed in transport solution and kept at 4°C for 5 hours, then prepared for evaluation. Malondialdehyde (MDA) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities were measured.

Results: MDA levels were significantly higher in IG and PCT than in CG and PCG. The activity of SOD was significantly lower in IG and higher in PCG than in the other groups. The activity of GPx was significantly lower in IG than in the other groups. The activities of CAT were significantly lower in IG and PCT than in the other groups and were significantly lower in IG than PCT. Histopathologic evaluation revealed normal findings in CG and PCG. While ischemic injury was observed in IG, the content of muscle fibers was better preserved in PCT.

Conclusions: Proanthocyanidin may have a protective effect on myocardial ischemic injury.

Show MeSH
Related in: MedlinePlus