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Activity of ozonated water and ozone against Staphylococcus aureus and Pseudomonas aeruginosa biofilms.

Bialoszewski D, Pietruczuk-Padzik A, Kalicinska A, Bocian E, Czajkowska M, Bukowska B, Tyski S - Med. Sci. Monit. (2011)

Bottom Line: The degree of reduction of viable bacteria following ozone exposure was determined.Ozonated water was found to be an effective bactericidal agent against biofilms after as little as 30 seconds of exposure, while the bactericidal activity of the ozone-oxygen solution was much lower.Unlike the ozone-oxygen mixture, ozonated water effectively destroys bacterial biofilms in vitro.<br />

View Article: PubMed Central - PubMed

Affiliation: Department of Physiotherapy, Second Medical Faculty, Medical University of Warsaw, Warsaw, Poland. dariusz.bialoszewski@wum.edu.pl

ABSTRACT

Background: The known bactericidal properties of ozone have not been checked in relation to its action on bacterial biofilms. This is especially true of ozonated fluids. The aim of this study was to investigate the bactericidal activity of ozonated water and that of a mixture of ozone and oxygen against biofilms.

Material/methods: Eighteen clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa exhibiting various levels of antibiotic sensitivity were investigated. Bacteria were cultured in biofilm form on polystyrene titration plates for periods of 2 to 72 hours. The biofilms formed in this way were exposed to in statu nascendi ozonated water produced in a prototype device that had been tested in clinical conditions, or to a mixture of oxygen and ozone generated in the same device. Live cells in the biofilm were stained with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide solution. The degree of reduction of viable bacteria following ozone exposure was determined.

Results: Ozonated water was found to be an effective bactericidal agent against biofilms after as little as 30 seconds of exposure, while the bactericidal activity of the ozone-oxygen solution was much lower. Prolongation of the duration of biofilm exposure to the gaseous disinfectant to 40 minutes led to a reduction in the viable cell count, which nevertheless remained high.

Conclusions: Unlike the ozone-oxygen mixture, ozonated water effectively destroys bacterial biofilms in vitro.

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Related in: MedlinePlus

Effect of ozonated water on biofilms of 9 strains of P. aeruginosa after 2, 24, 48 and 72 hours of incubation.
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f1-medscimonit-17-11-br339: Effect of ozonated water on biofilms of 9 strains of P. aeruginosa after 2, 24, 48 and 72 hours of incubation.

Mentions: P. aeruginosa strains formed biofilms on microtitration plates much earlier and much more vigorously than the S. aureus strains investigated in this study (Figure 1). After 2 hours of culturing at a temperature of 37°C, P. aeruginosa strains formed biofilms with different A554 absorbance values for different isolates, ranging from 0.3 to 1.1. At 24 hours of incubation, absorbance values rose for most strains, ranging from 0.5 to 1.2. At 48 and 72 hours, P. aeruginosa biofilms in nearly all strains demonstrated a gradual reduction in viable cell counts to absorbance levels of 0.3–0.9.


Activity of ozonated water and ozone against Staphylococcus aureus and Pseudomonas aeruginosa biofilms.

Bialoszewski D, Pietruczuk-Padzik A, Kalicinska A, Bocian E, Czajkowska M, Bukowska B, Tyski S - Med. Sci. Monit. (2011)

Effect of ozonated water on biofilms of 9 strains of P. aeruginosa after 2, 24, 48 and 72 hours of incubation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539485&req=5

f1-medscimonit-17-11-br339: Effect of ozonated water on biofilms of 9 strains of P. aeruginosa after 2, 24, 48 and 72 hours of incubation.
Mentions: P. aeruginosa strains formed biofilms on microtitration plates much earlier and much more vigorously than the S. aureus strains investigated in this study (Figure 1). After 2 hours of culturing at a temperature of 37°C, P. aeruginosa strains formed biofilms with different A554 absorbance values for different isolates, ranging from 0.3 to 1.1. At 24 hours of incubation, absorbance values rose for most strains, ranging from 0.5 to 1.2. At 48 and 72 hours, P. aeruginosa biofilms in nearly all strains demonstrated a gradual reduction in viable cell counts to absorbance levels of 0.3–0.9.

Bottom Line: The degree of reduction of viable bacteria following ozone exposure was determined.Ozonated water was found to be an effective bactericidal agent against biofilms after as little as 30 seconds of exposure, while the bactericidal activity of the ozone-oxygen solution was much lower.Unlike the ozone-oxygen mixture, ozonated water effectively destroys bacterial biofilms in vitro.<br />

View Article: PubMed Central - PubMed

Affiliation: Department of Physiotherapy, Second Medical Faculty, Medical University of Warsaw, Warsaw, Poland. dariusz.bialoszewski@wum.edu.pl

ABSTRACT

Background: The known bactericidal properties of ozone have not been checked in relation to its action on bacterial biofilms. This is especially true of ozonated fluids. The aim of this study was to investigate the bactericidal activity of ozonated water and that of a mixture of ozone and oxygen against biofilms.

Material/methods: Eighteen clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa exhibiting various levels of antibiotic sensitivity were investigated. Bacteria were cultured in biofilm form on polystyrene titration plates for periods of 2 to 72 hours. The biofilms formed in this way were exposed to in statu nascendi ozonated water produced in a prototype device that had been tested in clinical conditions, or to a mixture of oxygen and ozone generated in the same device. Live cells in the biofilm were stained with a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide solution. The degree of reduction of viable bacteria following ozone exposure was determined.

Results: Ozonated water was found to be an effective bactericidal agent against biofilms after as little as 30 seconds of exposure, while the bactericidal activity of the ozone-oxygen solution was much lower. Prolongation of the duration of biofilm exposure to the gaseous disinfectant to 40 minutes led to a reduction in the viable cell count, which nevertheless remained high.

Conclusions: Unlike the ozone-oxygen mixture, ozonated water effectively destroys bacterial biofilms in vitro.

Show MeSH
Related in: MedlinePlus