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Remote ischemic preconditioning protects neurocognitive function of rats following cerebral hypoperfusion.

Xu T, Gong Z, Zhu WZ, Wang JF, Li B, Chen F, Deng XM - Med. Sci. Monit. (2011)

Bottom Line: After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min.Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group).No apoptosis was observed on day 9.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Changhai Hospital, 2nd Military Medical University, Shanghai, P R China.

ABSTRACT

Background: Protection of remote ischemic preconditioning on neurocognitive function caused by bilateral common carotid artery occlusion has been investigated in rats.

Material/methods: Thirty-six male Sprague-Dawley rats were divided into 3 groups - control group (Group C, n=12), bilateral carotid arteries occlusion group (Group B, n=12) and remote ischemic precondition group (Group P, n=12). In Group P, remote ischemic preconditioning (RIPC) was performed on the right femoral artery with 3 cycles (10 min) of occlusion/perfusion. After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min. In Group B, ischemic insults were conducted without RIPC. Sham surgeries were performed in Group C. Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group). Apoptosis of cells in the hippocampus region was determined by TUNEL tests and Bcl-2 at this region was determined by ELISA 24 h and 9 days after vessel occlusion (n=6 for each group).

Results: Neurocognitive tests showed that latency time was significantly longer in Group B than in Group P on day 7 (p=0.016) and day 8 (p=0.036). Moreover, frequency of platform crossings was significant less in group B than in the other 2 groups on day 9. Bcl-2 level was significantly increased in the hippocampal region of rats in Group P on days 1 and 9 after vessel occlusion. TUNEL test showed that apoptosis could be observed at 24 h after occlusion in Group B, but not in Group P and Group C. No apoptosis was observed on day 9.

Conclusions: Our results suggest that RIPC can protect neurocognitive function of rats after bilateral carotid occlusions, and that Bcl-2 may play an important role in this protective effect.

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TUNEL assay in hippocampal CA1 region at 24 h after vessel occlusion.
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f3-medscimonit-17-11-br299: TUNEL assay in hippocampal CA1 region at 24 h after vessel occlusion.

Mentions: TUNEL assay showed that many apoptotic cells were present in Group B on day 1 after vessel occlusion, while there was hardly any apoptosis in the other 2 groups at the same time points (Figure 3). However, no apoptotic cells could be observed on day 9 after surgery (Figure 4).


Remote ischemic preconditioning protects neurocognitive function of rats following cerebral hypoperfusion.

Xu T, Gong Z, Zhu WZ, Wang JF, Li B, Chen F, Deng XM - Med. Sci. Monit. (2011)

TUNEL assay in hippocampal CA1 region at 24 h after vessel occlusion.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539484&req=5

f3-medscimonit-17-11-br299: TUNEL assay in hippocampal CA1 region at 24 h after vessel occlusion.
Mentions: TUNEL assay showed that many apoptotic cells were present in Group B on day 1 after vessel occlusion, while there was hardly any apoptosis in the other 2 groups at the same time points (Figure 3). However, no apoptotic cells could be observed on day 9 after surgery (Figure 4).

Bottom Line: After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min.Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group).No apoptosis was observed on day 9.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Changhai Hospital, 2nd Military Medical University, Shanghai, P R China.

ABSTRACT

Background: Protection of remote ischemic preconditioning on neurocognitive function caused by bilateral common carotid artery occlusion has been investigated in rats.

Material/methods: Thirty-six male Sprague-Dawley rats were divided into 3 groups - control group (Group C, n=12), bilateral carotid arteries occlusion group (Group B, n=12) and remote ischemic precondition group (Group P, n=12). In Group P, remote ischemic preconditioning (RIPC) was performed on the right femoral artery with 3 cycles (10 min) of occlusion/perfusion. After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min. In Group B, ischemic insults were conducted without RIPC. Sham surgeries were performed in Group C. Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group). Apoptosis of cells in the hippocampus region was determined by TUNEL tests and Bcl-2 at this region was determined by ELISA 24 h and 9 days after vessel occlusion (n=6 for each group).

Results: Neurocognitive tests showed that latency time was significantly longer in Group B than in Group P on day 7 (p=0.016) and day 8 (p=0.036). Moreover, frequency of platform crossings was significant less in group B than in the other 2 groups on day 9. Bcl-2 level was significantly increased in the hippocampal region of rats in Group P on days 1 and 9 after vessel occlusion. TUNEL test showed that apoptosis could be observed at 24 h after occlusion in Group B, but not in Group P and Group C. No apoptosis was observed on day 9.

Conclusions: Our results suggest that RIPC can protect neurocognitive function of rats after bilateral carotid occlusions, and that Bcl-2 may play an important role in this protective effect.

Show MeSH
Related in: MedlinePlus