Limits...
Remote ischemic preconditioning protects neurocognitive function of rats following cerebral hypoperfusion.

Xu T, Gong Z, Zhu WZ, Wang JF, Li B, Chen F, Deng XM - Med. Sci. Monit. (2011)

Bottom Line: After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min.Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group).No apoptosis was observed on day 9.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Changhai Hospital, 2nd Military Medical University, Shanghai, P R China.

ABSTRACT

Background: Protection of remote ischemic preconditioning on neurocognitive function caused by bilateral common carotid artery occlusion has been investigated in rats.

Material/methods: Thirty-six male Sprague-Dawley rats were divided into 3 groups - control group (Group C, n=12), bilateral carotid arteries occlusion group (Group B, n=12) and remote ischemic precondition group (Group P, n=12). In Group P, remote ischemic preconditioning (RIPC) was performed on the right femoral artery with 3 cycles (10 min) of occlusion/perfusion. After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min. In Group B, ischemic insults were conducted without RIPC. Sham surgeries were performed in Group C. Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group). Apoptosis of cells in the hippocampus region was determined by TUNEL tests and Bcl-2 at this region was determined by ELISA 24 h and 9 days after vessel occlusion (n=6 for each group).

Results: Neurocognitive tests showed that latency time was significantly longer in Group B than in Group P on day 7 (p=0.016) and day 8 (p=0.036). Moreover, frequency of platform crossings was significant less in group B than in the other 2 groups on day 9. Bcl-2 level was significantly increased in the hippocampal region of rats in Group P on days 1 and 9 after vessel occlusion. TUNEL test showed that apoptosis could be observed at 24 h after occlusion in Group B, but not in Group P and Group C. No apoptosis was observed on day 9.

Conclusions: Our results suggest that RIPC can protect neurocognitive function of rats after bilateral carotid occlusions, and that Bcl-2 may play an important role in this protective effect.

Show MeSH

Related in: MedlinePlus

Latency time of Morris water maze. Latency time in each group was not significantly different on day 5 and day 6. But it was significantly different on day 7 (p=0.016) and day 8 (p=0.036) between Group B and Group P, and on day 8 (p=0.047) between Group B and Group C. There were no significantly difference between Group C and Group P in any of the four testing days. * p<0.05 vs. Group B.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539484&req=5

f1-medscimonit-17-11-br299: Latency time of Morris water maze. Latency time in each group was not significantly different on day 5 and day 6. But it was significantly different on day 7 (p=0.016) and day 8 (p=0.036) between Group B and Group P, and on day 8 (p=0.047) between Group B and Group C. There were no significantly difference between Group C and Group P in any of the four testing days. * p<0.05 vs. Group B.

Mentions: Morris maze latencies and platform crossings are displayed in Figure 1 and 2, respectively. Latency time in each group was similar on days 5 and day 6, but it was significantly longer in Group B than in Group P on day 7 (p=0.016) and day 8 (p=0.036), and longer in Group B than in Group C on day 8 (p=0.047). There were no significant differences between Group C and Group P in any of the 4 testing days (Figure 1). Frequency of platform crossings was significantly lower in Group B than in the other 2 groups on day 9 (Figure 2).


Remote ischemic preconditioning protects neurocognitive function of rats following cerebral hypoperfusion.

Xu T, Gong Z, Zhu WZ, Wang JF, Li B, Chen F, Deng XM - Med. Sci. Monit. (2011)

Latency time of Morris water maze. Latency time in each group was not significantly different on day 5 and day 6. But it was significantly different on day 7 (p=0.016) and day 8 (p=0.036) between Group B and Group P, and on day 8 (p=0.047) between Group B and Group C. There were no significantly difference between Group C and Group P in any of the four testing days. * p<0.05 vs. Group B.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539484&req=5

f1-medscimonit-17-11-br299: Latency time of Morris water maze. Latency time in each group was not significantly different on day 5 and day 6. But it was significantly different on day 7 (p=0.016) and day 8 (p=0.036) between Group B and Group P, and on day 8 (p=0.047) between Group B and Group C. There were no significantly difference between Group C and Group P in any of the four testing days. * p<0.05 vs. Group B.
Mentions: Morris maze latencies and platform crossings are displayed in Figure 1 and 2, respectively. Latency time in each group was similar on days 5 and day 6, but it was significantly longer in Group B than in Group P on day 7 (p=0.016) and day 8 (p=0.036), and longer in Group B than in Group C on day 8 (p=0.047). There were no significant differences between Group C and Group P in any of the 4 testing days (Figure 1). Frequency of platform crossings was significantly lower in Group B than in the other 2 groups on day 9 (Figure 2).

Bottom Line: After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min.Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group).No apoptosis was observed on day 9.

View Article: PubMed Central - PubMed

Affiliation: Department of Anesthesiology and Intensive Care Medicine, Changhai Hospital, 2nd Military Medical University, Shanghai, P R China.

ABSTRACT

Background: Protection of remote ischemic preconditioning on neurocognitive function caused by bilateral common carotid artery occlusion has been investigated in rats.

Material/methods: Thirty-six male Sprague-Dawley rats were divided into 3 groups - control group (Group C, n=12), bilateral carotid arteries occlusion group (Group B, n=12) and remote ischemic precondition group (Group P, n=12). In Group P, remote ischemic preconditioning (RIPC) was performed on the right femoral artery with 3 cycles (10 min) of occlusion/perfusion. After 3 cycles of preconditioning, bilateral carotid arteries were occluded immediately for 60 min. In Group B, ischemic insults were conducted without RIPC. Sham surgeries were performed in Group C. Evaluation of memory and learning capacity was performed on days 5-8 after surgery by Morris water maze testing of spatial learning capacity (n=6 for each group). Apoptosis of cells in the hippocampus region was determined by TUNEL tests and Bcl-2 at this region was determined by ELISA 24 h and 9 days after vessel occlusion (n=6 for each group).

Results: Neurocognitive tests showed that latency time was significantly longer in Group B than in Group P on day 7 (p=0.016) and day 8 (p=0.036). Moreover, frequency of platform crossings was significant less in group B than in the other 2 groups on day 9. Bcl-2 level was significantly increased in the hippocampal region of rats in Group P on days 1 and 9 after vessel occlusion. TUNEL test showed that apoptosis could be observed at 24 h after occlusion in Group B, but not in Group P and Group C. No apoptosis was observed on day 9.

Conclusions: Our results suggest that RIPC can protect neurocognitive function of rats after bilateral carotid occlusions, and that Bcl-2 may play an important role in this protective effect.

Show MeSH
Related in: MedlinePlus