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Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.

Li MJ, Wang WW, Chen SW, Shen Q, Min R - Med. Sci. Monit. (2011)

Bottom Line: The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times.RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed.

View Article: PubMed Central - PubMed

Affiliation: Division of Radiation Medicine, Department of Naval Medicine, 2nd Military Medical University, Shanghai, China.

ABSTRACT

Background: The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.

Material/methods: Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).

Results: Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges.

Conclusions: The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.

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Related in: MedlinePlus

Relative values of IER5, GADD45A, PLK3, DDB2, BBC3 and ATM gene expression levels are plotted, and the dashed line indicates the non-irradiated control ratio. Data points denote the mean ±SD of 5 mice per treatment.
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f4-medscimonit-17-10-br290: Relative values of IER5, GADD45A, PLK3, DDB2, BBC3 and ATM gene expression levels are plotted, and the dashed line indicates the non-irradiated control ratio. Data points denote the mean ±SD of 5 mice per treatment.

Mentions: The changes in expression levels of these genes were upregulated and downregulated in an oscillating pattern across the observed time-points within certain dose ranges. To determine which genes could potentially characterize exposure doses at different post-irradiation time-points, the results are charted in Figure 4. Because the change in CDKN1A expression is substantial, it is not included in Figure 4. The summary of relative expression levels of genes, which are significantly different from the control group, is shown in Table 3. These changes may serve as a useful biomarker for exposure dose evaluation.


Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.

Li MJ, Wang WW, Chen SW, Shen Q, Min R - Med. Sci. Monit. (2011)

Relative values of IER5, GADD45A, PLK3, DDB2, BBC3 and ATM gene expression levels are plotted, and the dashed line indicates the non-irradiated control ratio. Data points denote the mean ±SD of 5 mice per treatment.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539470&req=5

f4-medscimonit-17-10-br290: Relative values of IER5, GADD45A, PLK3, DDB2, BBC3 and ATM gene expression levels are plotted, and the dashed line indicates the non-irradiated control ratio. Data points denote the mean ±SD of 5 mice per treatment.
Mentions: The changes in expression levels of these genes were upregulated and downregulated in an oscillating pattern across the observed time-points within certain dose ranges. To determine which genes could potentially characterize exposure doses at different post-irradiation time-points, the results are charted in Figure 4. Because the change in CDKN1A expression is substantial, it is not included in Figure 4. The summary of relative expression levels of genes, which are significantly different from the control group, is shown in Table 3. These changes may serve as a useful biomarker for exposure dose evaluation.

Bottom Line: The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times.RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed.

View Article: PubMed Central - PubMed

Affiliation: Division of Radiation Medicine, Department of Naval Medicine, 2nd Military Medical University, Shanghai, China.

ABSTRACT

Background: The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.

Material/methods: Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).

Results: Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges.

Conclusions: The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.

Show MeSH
Related in: MedlinePlus