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Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.

Li MJ, Wang WW, Chen SW, Shen Q, Min R - Med. Sci. Monit. (2011)

Bottom Line: The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times.RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed.

View Article: PubMed Central - PubMed

Affiliation: Division of Radiation Medicine, Department of Naval Medicine, 2nd Military Medical University, Shanghai, China.

ABSTRACT

Background: The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.

Material/methods: Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).

Results: Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges.

Conclusions: The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.

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Related in: MedlinePlus

The dose responses of the expression levels of 6 genes after radiation exposure are plotted. The points denote the mean responses for 5 different mice; error bars denote SD.
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f3-medscimonit-17-10-br290: The dose responses of the expression levels of 6 genes after radiation exposure are plotted. The points denote the mean responses for 5 different mice; error bars denote SD.

Mentions: The genes BBC3, XPC, PLK3, RAD50, DDB2, FDXR, GADD45A, BRCA1 and IER5 are largely involved in DNA repair or cell cycle regulation associated with DNA repair. The results show that genes BRCA1, XPC and FDXR had no significant change in expression level post-irradiation across all observed dose ranges and post-irradiation time-points. The time- and dose-dependent responses of 6 other genes (BBC3, PLK3, DDB2, GADD45A, RAD50 and IER5) are shown in Figure 3.


Radiation dose effect of DNA repair-related gene expression in mouse white blood cells.

Li MJ, Wang WW, Chen SW, Shen Q, Min R - Med. Sci. Monit. (2011)

The dose responses of the expression levels of 6 genes after radiation exposure are plotted. The points denote the mean responses for 5 different mice; error bars denote SD.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539470&req=5

f3-medscimonit-17-10-br290: The dose responses of the expression levels of 6 genes after radiation exposure are plotted. The points denote the mean responses for 5 different mice; error bars denote SD.
Mentions: The genes BBC3, XPC, PLK3, RAD50, DDB2, FDXR, GADD45A, BRCA1 and IER5 are largely involved in DNA repair or cell cycle regulation associated with DNA repair. The results show that genes BRCA1, XPC and FDXR had no significant change in expression level post-irradiation across all observed dose ranges and post-irradiation time-points. The time- and dose-dependent responses of 6 other genes (BBC3, PLK3, DDB2, GADD45A, RAD50 and IER5) are shown in Figure 3.

Bottom Line: The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times.RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed.

View Article: PubMed Central - PubMed

Affiliation: Division of Radiation Medicine, Department of Naval Medicine, 2nd Military Medical University, Shanghai, China.

ABSTRACT

Background: The aim of this study was to screen molecular biomarkers for biodosimetry from DNA repair-related gene expression profiles.

Material/methods: Mice were subjected to whole-body exposure with 60Co gamma rays with a dose range of 0-8 Gy at a dose rate of 0.80 Gy/min. RNA was extracted from the peripheral blood of irradiated mice at 4, 8, 12, 24 and 48hrs post-irradiation. The mRNA transcriptional changes of 11 genes related to DNA damage and repair were detected using real-time quantitative polymerase chain reaction (RT-PCR).

Results: Of the 11 genes examined, CDKN1A (cyclin-dependent kinase inhibitor 1A or p21, Cip1) and ATM (ataxia telangiectasia mutated) expression levels were found to be heavily up- and down-regulated, respectively, with exposure dose increasing at different post-irradiation times. RAD50 (RAD50 homolog), PLK3 (polo-like kinase 3), GADD45A (growth arrest and DNA damage-inducible, alpha), DDB2 (damage-specific DNA-binding protein 2), BBC3 (BCL2-binding component 3) and IER5 (immediate early response 5) gene expression levels were found to undergo significant oscillating changes over a broad dose range of 2-8 Gy at post-exposure time points observed. Three of the genes were found not to change within the observed exposure dose and post-radiation time ranges.

Conclusions: The results of this study add to the biodosimetry with biomarker data pool and will be helpful for constructing appropriate gene expression biomarker systems to evaluate radiation exposure doses.

Show MeSH
Related in: MedlinePlus