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The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

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Related in: MedlinePlus

Spectrum of background (morphotic elements of tissue excretion) – matrix effect.
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f5-medscimonit-17-10-mt91: Spectrum of background (morphotic elements of tissue excretion) – matrix effect.

Mentions: The typical peak characterizing presence of E. coli appeared as a single peak in time range from 2 to 5 minutes within flat-wide, complex and irregular signal (matrix-effect). Such a single high signal in the electropherogram was considered a pathognomonic picture for E. coli infection, as well as for cases of complex infections (Figures 3,4). Matrix effect being an irregular, wide signal, contained in 0–15 minutes band, was a picture of sample background, which in this case consisted of solid particles and chemical substances of tissue excretion (eg, epithelial cells, morphotic elements of blood, fibrin, minerals) (Figure 5).


The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Spectrum of background (morphotic elements of tissue excretion) – matrix effect.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539466&req=5

f5-medscimonit-17-10-mt91: Spectrum of background (morphotic elements of tissue excretion) – matrix effect.
Mentions: The typical peak characterizing presence of E. coli appeared as a single peak in time range from 2 to 5 minutes within flat-wide, complex and irregular signal (matrix-effect). Such a single high signal in the electropherogram was considered a pathognomonic picture for E. coli infection, as well as for cases of complex infections (Figures 3,4). Matrix effect being an irregular, wide signal, contained in 0–15 minutes band, was a picture of sample background, which in this case consisted of solid particles and chemical substances of tissue excretion (eg, epithelial cells, morphotic elements of blood, fibrin, minerals) (Figure 5).

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

Show MeSH
Related in: MedlinePlus