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The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

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Related in: MedlinePlus

Typical electropherograms of biological sample (A, B) and standard (C) with presence of Escherichia coli (arrow).
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getmorefigures.php?uid=PMC3539466&req=5

f3-medscimonit-17-10-mt91: Typical electropherograms of biological sample (A, B) and standard (C) with presence of Escherichia coli (arrow).

Mentions: Based on the final analyzed 52 (2*26) biological samples of isolated infections, a standard electropherogram that characterized presence of bacterial cells of Escherichia coli was determined (Figure 3).


The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Typical electropherograms of biological sample (A, B) and standard (C) with presence of Escherichia coli (arrow).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539466&req=5

f3-medscimonit-17-10-mt91: Typical electropherograms of biological sample (A, B) and standard (C) with presence of Escherichia coli (arrow).
Mentions: Based on the final analyzed 52 (2*26) biological samples of isolated infections, a standard electropherogram that characterized presence of bacterial cells of Escherichia coli was determined (Figure 3).

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

Show MeSH
Related in: MedlinePlus