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The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

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Related in: MedlinePlus

Electropherogram of additional strains in the biological sample, in which conventional method primarily detected only presence of E. coli. The next inoculation showed co-infection of Staphylococcus sp.
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f2-medscimonit-17-10-mt91: Electropherogram of additional strains in the biological sample, in which conventional method primarily detected only presence of E. coli. The next inoculation showed co-infection of Staphylococcus sp.

Mentions: In the final analyzed group of 30 infections with E. coli, in 26 cases pathogen was concomitantly diagnosed within as few as 45 minutes, based only on pathognomonic electropherogram of the sample. The picture was ambiguous in 4 cases, not allowing a reliable diagnosis. Interestingly, in as many as in 3 of them, based on the character of the obtained CZE diagram, a concomitant infection with cocci forming clusters was diagnosed with a high probability. On the following day classical inoculations from these 3 wounds were repeated. In the obtained, repeated results, concomitant infections with Staphylococcus sp. strains (2) or Enterococcus sp. (1) were confirmed in all 3 cases (Figure 2). All of these cases were also rejected from further processing for E. coli due to the risk of interaction of bacterial cells of various species under conditions of CZE. A similar, but opposite situation was observed in a group of 20 infections with bacteria different from E. coli. In these 3 cases, coexistence of signal typical for E. coli was recognized by the CZE method; however, it was not confirmed primarily by a reference method, but in this case, subsequent verifying inoculations were not carried out.


The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Electropherogram of additional strains in the biological sample, in which conventional method primarily detected only presence of E. coli. The next inoculation showed co-infection of Staphylococcus sp.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539466&req=5

f2-medscimonit-17-10-mt91: Electropherogram of additional strains in the biological sample, in which conventional method primarily detected only presence of E. coli. The next inoculation showed co-infection of Staphylococcus sp.
Mentions: In the final analyzed group of 30 infections with E. coli, in 26 cases pathogen was concomitantly diagnosed within as few as 45 minutes, based only on pathognomonic electropherogram of the sample. The picture was ambiguous in 4 cases, not allowing a reliable diagnosis. Interestingly, in as many as in 3 of them, based on the character of the obtained CZE diagram, a concomitant infection with cocci forming clusters was diagnosed with a high probability. On the following day classical inoculations from these 3 wounds were repeated. In the obtained, repeated results, concomitant infections with Staphylococcus sp. strains (2) or Enterococcus sp. (1) were confirmed in all 3 cases (Figure 2). All of these cases were also rejected from further processing for E. coli due to the risk of interaction of bacterial cells of various species under conditions of CZE. A similar, but opposite situation was observed in a group of 20 infections with bacteria different from E. coli. In these 3 cases, coexistence of signal typical for E. coli was recognized by the CZE method; however, it was not confirmed primarily by a reference method, but in this case, subsequent verifying inoculations were not carried out.

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

Show MeSH
Related in: MedlinePlus