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The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

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Related in: MedlinePlus

Example of an infected wound (appendectomy).
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f1-medscimonit-17-10-mt91: Example of an infected wound (appendectomy).

Mentions: Each of the 60 sites from which microbiological test material was taken was catalogued. In 31 subjects these were infected postoperative wounds after resection of the large and the small intestine (group A), in 8 subjects they were trophic ulceration of the lower extremity due to chronic venous insufficiency or ischemia (group B), in 7 patients specimens were taken from infected wounds after urgent laparotomy in the course of peritonitis following perforation of the gastrointestinal tract (group C), in 10 subjects they were from suppurative wounds following other surgical procedures of the alimentary tract without affecting the intestinal continuity (eg, infected necrosis of the pancreas in acute pancreatitis) (group D) and in 4 subjects they were from foot ulceration in diabetic foot syndrome (group E). An example of a wound is presented in Figure 1.


The clinical use of a fast screening test based on technology of capillary zone electrophoresis (CZE) for identification of Escherichia coli infection in biological material.

Szeliga J, Klodzinska E, Jackowski M, Buszewski B - Med. Sci. Monit. (2011)

Example of an infected wound (appendectomy).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539466&req=5

f1-medscimonit-17-10-mt91: Example of an infected wound (appendectomy).
Mentions: Each of the 60 sites from which microbiological test material was taken was catalogued. In 31 subjects these were infected postoperative wounds after resection of the large and the small intestine (group A), in 8 subjects they were trophic ulceration of the lower extremity due to chronic venous insufficiency or ischemia (group B), in 7 patients specimens were taken from infected wounds after urgent laparotomy in the course of peritonitis following perforation of the gastrointestinal tract (group C), in 10 subjects they were from suppurative wounds following other surgical procedures of the alimentary tract without affecting the intestinal continuity (eg, infected necrosis of the pancreas in acute pancreatitis) (group D) and in 4 subjects they were from foot ulceration in diabetic foot syndrome (group E). An example of a wound is presented in Figure 1.

Bottom Line: Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour.The obtained results enable reliable, very fast testing for E. coli as a pathogen.

View Article: PubMed Central - PubMed

Affiliation: Department of General, Gastroenterological and Oncological Surgery, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland.

ABSTRACT

Background: Escherichia coli is a Gram-negative bacterium which is a basic, symbiotic element of the physiological flora of the large intestine of humans and warm-blooded animals. However, in specific cases it may become a very dangerous pathogen (eg, diarrhoea, infection of the urinary tract, lungs, and generalized infections). Its early detection, as a cause of infectious disease, helps to achieve optimal treatment results; however, classical microbiological tests require at least 24 hours from sample taking to diagnosis.

Material/methods: We present a unique solution based on CZE technologies enabling identification of E. coli presence in studied sample within half an hour. Altogether, 30 E. coli-infected wounds and ulcerations were examined, comparing the results obtained by classical culture method with the result of capillary zone electrophoresis (CZE) electropherogram.

Results: The method, which does not require any preparation of the sample, achieved 86.7% sensitivity and 85%specificity in the examined clinical material (infections of surgical wounds).

Conclusions: The obtained results enable reliable, very fast testing for E. coli as a pathogen.

Show MeSH
Related in: MedlinePlus