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Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

Sineh Sepehr K, Baradaran B, Mazandarani M, Khori V, Shahneh FZ - ISRN Pharm (2012)

Bottom Line: Dye exclusion analysis was performed for viability rate.Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells.The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

View Article: PubMed Central - PubMed

Affiliation: Immunology Research Center (IRC), University of Medical Sciences Tabriz, Iran.

ABSTRACT
The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

No MeSH data available.


Related in: MedlinePlus

Nuclei morphological changes during Punica granatum spinosa induced apoptosis in PC3 cells detected by TUNEL assay. Tumor cells treated with extract (250 μg/mL) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, (a) treated with extract (250 μg) for 24 h and (b) show negative control (without treatment).
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fig4: Nuclei morphological changes during Punica granatum spinosa induced apoptosis in PC3 cells detected by TUNEL assay. Tumor cells treated with extract (250 μg/mL) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, (a) treated with extract (250 μg) for 24 h and (b) show negative control (without treatment).

Mentions: To test whether or not peel extract that induced the decrease of cell viability and cytotoxicity contributes to apoptotic death in PC3 cell lines in vitro. Cells were incubated with 250 μg/mL of PGS for 24 h and then determined using TUNEL assay. It was found that the PC3 cells treated with peel extract (250 μg/mL) for 24 h exhibited apoptotic body formation (Figure 4). PC3 cells treated with peel extract displayed typical morphological features of apoptotic cells, with condensed and fragmented nuclei (Figure 4(a)). However, homogenous nuclear chromatin was evident in control cells (Figure 4(b)). The induction of apoptosis by peel extract was confirmed by in situ TUNEL assay. TUNEL assay based on labeling of DNA strand breaks generated during apoptosis revealed that peel extract induces apoptosis in PC3 cells.


Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

Sineh Sepehr K, Baradaran B, Mazandarani M, Khori V, Shahneh FZ - ISRN Pharm (2012)

Nuclei morphological changes during Punica granatum spinosa induced apoptosis in PC3 cells detected by TUNEL assay. Tumor cells treated with extract (250 μg/mL) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, (a) treated with extract (250 μg) for 24 h and (b) show negative control (without treatment).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539436&req=5

fig4: Nuclei morphological changes during Punica granatum spinosa induced apoptosis in PC3 cells detected by TUNEL assay. Tumor cells treated with extract (250 μg/mL) were assayed by TUNEL and observed under light microscopy. For PC3 cells group, (a) treated with extract (250 μg) for 24 h and (b) show negative control (without treatment).
Mentions: To test whether or not peel extract that induced the decrease of cell viability and cytotoxicity contributes to apoptotic death in PC3 cell lines in vitro. Cells were incubated with 250 μg/mL of PGS for 24 h and then determined using TUNEL assay. It was found that the PC3 cells treated with peel extract (250 μg/mL) for 24 h exhibited apoptotic body formation (Figure 4). PC3 cells treated with peel extract displayed typical morphological features of apoptotic cells, with condensed and fragmented nuclei (Figure 4(a)). However, homogenous nuclear chromatin was evident in control cells (Figure 4(b)). The induction of apoptosis by peel extract was confirmed by in situ TUNEL assay. TUNEL assay based on labeling of DNA strand breaks generated during apoptosis revealed that peel extract induces apoptosis in PC3 cells.

Bottom Line: Dye exclusion analysis was performed for viability rate.Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells.The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

View Article: PubMed Central - PubMed

Affiliation: Immunology Research Center (IRC), University of Medical Sciences Tabriz, Iran.

ABSTRACT
The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

No MeSH data available.


Related in: MedlinePlus