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Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

Sineh Sepehr K, Baradaran B, Mazandarani M, Khori V, Shahneh FZ - ISRN Pharm (2012)

Bottom Line: Dye exclusion analysis was performed for viability rate.Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells.The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

View Article: PubMed Central - PubMed

Affiliation: Immunology Research Center (IRC), University of Medical Sciences Tabriz, Iran.

ABSTRACT
The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

No MeSH data available.


Related in: MedlinePlus

Results are expressed as the mean percentage of viable cells with 3 wells each. Percentage of viable cells was calculated from the ratio of viable cells to total number of cells using trypan blue exclusion test.
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fig2: Results are expressed as the mean percentage of viable cells with 3 wells each. Percentage of viable cells was calculated from the ratio of viable cells to total number of cells using trypan blue exclusion test.

Mentions: In 24 and 48 h Dye exclusion assay evaluated viability of PC3 cells exposed to peel extract. As the result in Figure 2, the viabilities of cells exposed to peel PGS extract at concentrations of 10 and 600 μg/mL were 96.3 ± 7.8% and 24.1 ± 2.5%, respectively.


Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

Sineh Sepehr K, Baradaran B, Mazandarani M, Khori V, Shahneh FZ - ISRN Pharm (2012)

Results are expressed as the mean percentage of viable cells with 3 wells each. Percentage of viable cells was calculated from the ratio of viable cells to total number of cells using trypan blue exclusion test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539436&req=5

fig2: Results are expressed as the mean percentage of viable cells with 3 wells each. Percentage of viable cells was calculated from the ratio of viable cells to total number of cells using trypan blue exclusion test.
Mentions: In 24 and 48 h Dye exclusion assay evaluated viability of PC3 cells exposed to peel extract. As the result in Figure 2, the viabilities of cells exposed to peel PGS extract at concentrations of 10 and 600 μg/mL were 96.3 ± 7.8% and 24.1 ± 2.5%, respectively.

Bottom Line: Dye exclusion analysis was performed for viability rate.Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells.The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

View Article: PubMed Central - PubMed

Affiliation: Immunology Research Center (IRC), University of Medical Sciences Tabriz, Iran.

ABSTRACT
The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

No MeSH data available.


Related in: MedlinePlus