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Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

Sineh Sepehr K, Baradaran B, Mazandarani M, Khori V, Shahneh FZ - ISRN Pharm (2012)

Bottom Line: Dye exclusion analysis was performed for viability rate.Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells.The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

View Article: PubMed Central - PubMed

Affiliation: Immunology Research Center (IRC), University of Medical Sciences Tabriz, Iran.

ABSTRACT
The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

No MeSH data available.


Related in: MedlinePlus

Effects of Punica granatum spinosa peel extract with increasing concentrations (10–600 μg/mL with) on proliferation of PC3 cells (a), seed extract on PC3 cells (b), and peel extract on L929 cells (c) for 24 h and 48 h the proliferative response was assessed by MTT assay.
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fig1: Effects of Punica granatum spinosa peel extract with increasing concentrations (10–600 μg/mL with) on proliferation of PC3 cells (a), seed extract on PC3 cells (b), and peel extract on L929 cells (c) for 24 h and 48 h the proliferative response was assessed by MTT assay.

Mentions: Peels of PGS extract at 10 to 600 μg/mL exhibited significant dose-dependent inhibitory effects on the proliferation of PC3. Growth inhibition of peel extract in 24 and 48 h was 61.2 ± 2.3% and 67.±1.75%, respectively (Figure 1(a)), with more than 75% suppression. The concentrations producing 50% growth inhibition (IC50) of the PGS extract on PC3 were effectively suppressed with the IC50 value (250.21 μg/mL) after incubation with the peel extract. However seed extract induced no significant suppression on the proliferation of PC3 cells (Figure 1(b)) and the peel extract induced no significant suppression on the proliferation of normal L929 cells (Figure 1(c)). PC3 cells were compared to elucidate the cytotoxicity of both peels of PGS extract and Toxol (chemotherapeutic agent, control positive) with more than 75% in 600 μg/mL and 90% in 20 μg/mL growth suppression in 24 h (Table 1).


Studies on the Cytotoxic Activities of Punica granatum L. var. spinosa (Apple Punice) Extract on Prostate Cell Line by Induction of Apoptosis.

Sineh Sepehr K, Baradaran B, Mazandarani M, Khori V, Shahneh FZ - ISRN Pharm (2012)

Effects of Punica granatum spinosa peel extract with increasing concentrations (10–600 μg/mL with) on proliferation of PC3 cells (a), seed extract on PC3 cells (b), and peel extract on L929 cells (c) for 24 h and 48 h the proliferative response was assessed by MTT assay.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539436&req=5

fig1: Effects of Punica granatum spinosa peel extract with increasing concentrations (10–600 μg/mL with) on proliferation of PC3 cells (a), seed extract on PC3 cells (b), and peel extract on L929 cells (c) for 24 h and 48 h the proliferative response was assessed by MTT assay.
Mentions: Peels of PGS extract at 10 to 600 μg/mL exhibited significant dose-dependent inhibitory effects on the proliferation of PC3. Growth inhibition of peel extract in 24 and 48 h was 61.2 ± 2.3% and 67.±1.75%, respectively (Figure 1(a)), with more than 75% suppression. The concentrations producing 50% growth inhibition (IC50) of the PGS extract on PC3 were effectively suppressed with the IC50 value (250.21 μg/mL) after incubation with the peel extract. However seed extract induced no significant suppression on the proliferation of PC3 cells (Figure 1(b)) and the peel extract induced no significant suppression on the proliferation of normal L929 cells (Figure 1(c)). PC3 cells were compared to elucidate the cytotoxicity of both peels of PGS extract and Toxol (chemotherapeutic agent, control positive) with more than 75% in 600 μg/mL and 90% in 20 μg/mL growth suppression in 24 h (Table 1).

Bottom Line: Dye exclusion analysis was performed for viability rate.Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells.The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

View Article: PubMed Central - PubMed

Affiliation: Immunology Research Center (IRC), University of Medical Sciences Tabriz, Iran.

ABSTRACT
The Punica granatum L. var. granatum (pomegranate) has been demonstrated to exert antitumor effects on various types of cancer cells. The present study aimed to evaluate the medicinal herbs Punica granatum L. var. spinosa (apple punice) that are native to Iran. This study was determined to test the possible cytotoxic activity and induction of apoptosis on human prostate cell lines. The effect of ethanol extracts of the herbs on the inhibition of cell proliferation was assessed by MTT colorimetric assay. PC3 cell lines treated with the extracts were analyzed for the induction of apoptosis by cell death detection (ELISA) and TUNEL assay. Dye exclusion analysis was performed for viability rate. Our results demonstrated that the Punica granatum L. var. spinosa extract dose dependently suppressed the proliferation of PC3 cells (IC(50)= 250.21 μg/mL) when compared with a chemotherapeutic anticancer drug (Toxol) (Vesper Pharmaceuticals) with increased nucleosome production from apoptotic cells. The Punica granatum L. var. spinosa extract attenuated the human prostate cell proliferation in vitro possibly by inducing apoptosis. The Punica granatum L. var. spinosa is likely to be valuable for the treatment of some forms of human prostate cell line.

No MeSH data available.


Related in: MedlinePlus