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Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus

(a) Chromatogram of fraction HE-Bu detected by PDA 360 nm. All peaks had absorbance curves characteristic of flavonols (b). (c) Schematic representation of the homolytic cleavage on CID-MS of peaks 7 (d) and 12 (e) identified as myricetin and quercetin rhamnosides, respectively.
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fig4: (a) Chromatogram of fraction HE-Bu detected by PDA 360 nm. All peaks had absorbance curves characteristic of flavonols (b). (c) Schematic representation of the homolytic cleavage on CID-MS of peaks 7 (d) and 12 (e) identified as myricetin and quercetin rhamnosides, respectively.

Mentions: Considering that the overwhelming inflammatory and immune responses during the early stage of sepsis involve a vast array of mediators, it is critical to control this complex inflammatory cascade and consequently to manage the sepsis effects [17]. Thus, natural products containing various components that act in different inflammatory cascades may have an advantage on sepsis treatment when administered in association with conventional drugs which target a single mediator. In this study, many flavonoids were found in the HE-Bu from E. uniflora leaves, consisted mainly by glycosides of quercetin and myricetin, by using the offline ESI-MS (Figure 4 and Table 1).


Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

(a) Chromatogram of fraction HE-Bu detected by PDA 360 nm. All peaks had absorbance curves characteristic of flavonols (b). (c) Schematic representation of the homolytic cleavage on CID-MS of peaks 7 (d) and 12 (e) identified as myricetin and quercetin rhamnosides, respectively.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539388&req=5

fig4: (a) Chromatogram of fraction HE-Bu detected by PDA 360 nm. All peaks had absorbance curves characteristic of flavonols (b). (c) Schematic representation of the homolytic cleavage on CID-MS of peaks 7 (d) and 12 (e) identified as myricetin and quercetin rhamnosides, respectively.
Mentions: Considering that the overwhelming inflammatory and immune responses during the early stage of sepsis involve a vast array of mediators, it is critical to control this complex inflammatory cascade and consequently to manage the sepsis effects [17]. Thus, natural products containing various components that act in different inflammatory cascades may have an advantage on sepsis treatment when administered in association with conventional drugs which target a single mediator. In this study, many flavonoids were found in the HE-Bu from E. uniflora leaves, consisted mainly by glycosides of quercetin and myricetin, by using the offline ESI-MS (Figure 4 and Table 1).

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus