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Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus

HE-Bu from E. uniflora inhibits iNOS (b) and COX-2 (c) expression in ileum of infected mice. Mice were treated with HE-Bu 75, 150, or 300 mg·kg−1, p.o. or dexamethasone. The levels of iNOS and COX-2 were determined by western blot analysis. Representative immunoblots (a). Results are shown as the means ± SEM of 2 to 3 different experiments. ###P < 0.001, CLP plus vehicle versus sham. **P < 0.01 and ***P < 0.001, HE-Bu versus vehicle.
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fig3: HE-Bu from E. uniflora inhibits iNOS (b) and COX-2 (c) expression in ileum of infected mice. Mice were treated with HE-Bu 75, 150, or 300 mg·kg−1, p.o. or dexamethasone. The levels of iNOS and COX-2 were determined by western blot analysis. Representative immunoblots (a). Results are shown as the means ± SEM of 2 to 3 different experiments. ###P < 0.001, CLP plus vehicle versus sham. **P < 0.01 and ***P < 0.001, HE-Bu versus vehicle.

Mentions: In this study, we examined the effects of HE-Bu, by immunoblotting, on iNOS and COX-2 expression by ileum cells of septic mice. HE-Bu (75, 150, and 300 mg·kg−1) decreased the levels of iNOS by 35.2%, 33.5%, and 75.5% respectively (Figures 3(a) and 3(b)). The COX-2 expression was reduced by 12.7, and 62% after treating the animals with the higher doses of HE-Bu. Dexamethasone significantly affected both iNOS and COX-2 expression, reducing by 69.5% and 80.8%, respectively (Figures 3(a) and 3(c)). These results clearly indicated that HE-Bu is able to decrease the levels of both investigated proinflammatory enzymes (iNOS and COX-2) whose role is increasingly recognized in the pathophysiology of sepsis.


Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

HE-Bu from E. uniflora inhibits iNOS (b) and COX-2 (c) expression in ileum of infected mice. Mice were treated with HE-Bu 75, 150, or 300 mg·kg−1, p.o. or dexamethasone. The levels of iNOS and COX-2 were determined by western blot analysis. Representative immunoblots (a). Results are shown as the means ± SEM of 2 to 3 different experiments. ###P < 0.001, CLP plus vehicle versus sham. **P < 0.01 and ***P < 0.001, HE-Bu versus vehicle.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539388&req=5

fig3: HE-Bu from E. uniflora inhibits iNOS (b) and COX-2 (c) expression in ileum of infected mice. Mice were treated with HE-Bu 75, 150, or 300 mg·kg−1, p.o. or dexamethasone. The levels of iNOS and COX-2 were determined by western blot analysis. Representative immunoblots (a). Results are shown as the means ± SEM of 2 to 3 different experiments. ###P < 0.001, CLP plus vehicle versus sham. **P < 0.01 and ***P < 0.001, HE-Bu versus vehicle.
Mentions: In this study, we examined the effects of HE-Bu, by immunoblotting, on iNOS and COX-2 expression by ileum cells of septic mice. HE-Bu (75, 150, and 300 mg·kg−1) decreased the levels of iNOS by 35.2%, 33.5%, and 75.5% respectively (Figures 3(a) and 3(b)). The COX-2 expression was reduced by 12.7, and 62% after treating the animals with the higher doses of HE-Bu. Dexamethasone significantly affected both iNOS and COX-2 expression, reducing by 69.5% and 80.8%, respectively (Figures 3(a) and 3(c)). These results clearly indicated that HE-Bu is able to decrease the levels of both investigated proinflammatory enzymes (iNOS and COX-2) whose role is increasingly recognized in the pathophysiology of sepsis.

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus