Limits...
Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus

Effect of HE-Bu obtained from Eugenia uniflora upon TNF-α (a) and IL-1β (b) production in infected mice serum. The mice, except the sham group, received vehicle, HE-Bu 75, 150. and 300 mg·kg−1 (p.o.) or dexamethasone (0.5 mg·kg−1 s.c.), which were administered 1 h before CLP surgery, and the cytokines levels were evaluated 6 h after onset. Each group represents the mean ± SEM of four to five animals. *P < 0.05, **P < 0.01, and ***P < 0.001 indicated value versus CLP plus vehicle group; ##P < 0.01 and ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. A false-operated group (sham) was also provided for this test.
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3539388&req=5

fig2: Effect of HE-Bu obtained from Eugenia uniflora upon TNF-α (a) and IL-1β (b) production in infected mice serum. The mice, except the sham group, received vehicle, HE-Bu 75, 150. and 300 mg·kg−1 (p.o.) or dexamethasone (0.5 mg·kg−1 s.c.), which were administered 1 h before CLP surgery, and the cytokines levels were evaluated 6 h after onset. Each group represents the mean ± SEM of four to five animals. *P < 0.05, **P < 0.01, and ***P < 0.001 indicated value versus CLP plus vehicle group; ##P < 0.01 and ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. A false-operated group (sham) was also provided for this test.

Mentions: Several previous studies have demonstrated that some cytokines, especially TNF-α and IL-1β are strongly associated with sepsis syndrome; therefore, inhibiting the proinflammatory cytokine overproduction during early sepsis may reduce its risks [17]. In this study, the HE-Bu was able to decrease significantly TNF-α and also IL-1β serum levels (Figures 2(a) and 2(b)), and it may be related to the mortality reduction.


Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

Effect of HE-Bu obtained from Eugenia uniflora upon TNF-α (a) and IL-1β (b) production in infected mice serum. The mice, except the sham group, received vehicle, HE-Bu 75, 150. and 300 mg·kg−1 (p.o.) or dexamethasone (0.5 mg·kg−1 s.c.), which were administered 1 h before CLP surgery, and the cytokines levels were evaluated 6 h after onset. Each group represents the mean ± SEM of four to five animals. *P < 0.05, **P < 0.01, and ***P < 0.001 indicated value versus CLP plus vehicle group; ##P < 0.01 and ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. A false-operated group (sham) was also provided for this test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539388&req=5

fig2: Effect of HE-Bu obtained from Eugenia uniflora upon TNF-α (a) and IL-1β (b) production in infected mice serum. The mice, except the sham group, received vehicle, HE-Bu 75, 150. and 300 mg·kg−1 (p.o.) or dexamethasone (0.5 mg·kg−1 s.c.), which were administered 1 h before CLP surgery, and the cytokines levels were evaluated 6 h after onset. Each group represents the mean ± SEM of four to five animals. *P < 0.05, **P < 0.01, and ***P < 0.001 indicated value versus CLP plus vehicle group; ##P < 0.01 and ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. A false-operated group (sham) was also provided for this test.
Mentions: Several previous studies have demonstrated that some cytokines, especially TNF-α and IL-1β are strongly associated with sepsis syndrome; therefore, inhibiting the proinflammatory cytokine overproduction during early sepsis may reduce its risks [17]. In this study, the HE-Bu was able to decrease significantly TNF-α and also IL-1β serum levels (Figures 2(a) and 2(b)), and it may be related to the mortality reduction.

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus