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Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus

HE-Bu obtained from Eugenia uniflora leaves protects against sepsis-induced lethality and inhibits myeloperoxidase activity (measured after 6 h postoperation). Mice (10 animals/group) were orally administered various doses of HE-Bu (75, 150 or 300 mg·kg−1), vehicle (3% ethanol), or dexamethasone (0.5 mg·kg−1 s.c.). MPO graph: values represent means ± SEM. ***P < 0.001, and **P < 0.01, indicated value versus CLP plus vehicle group; ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. Survival graph: survival analyses were compared by a logrank test. These calculations were performed with SigmaStat v3.10 (Systat Software Inc, Richmond, CA, USA). The  hypothesis was rejected when P < 0.05.
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fig1: HE-Bu obtained from Eugenia uniflora leaves protects against sepsis-induced lethality and inhibits myeloperoxidase activity (measured after 6 h postoperation). Mice (10 animals/group) were orally administered various doses of HE-Bu (75, 150 or 300 mg·kg−1), vehicle (3% ethanol), or dexamethasone (0.5 mg·kg−1 s.c.). MPO graph: values represent means ± SEM. ***P < 0.001, and **P < 0.01, indicated value versus CLP plus vehicle group; ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. Survival graph: survival analyses were compared by a logrank test. These calculations were performed with SigmaStat v3.10 (Systat Software Inc, Richmond, CA, USA). The hypothesis was rejected when P < 0.05.

Mentions: It was observed that lethality was markedly delayed in mice orally administered with HE-Bu (see Figure 1(a)). Mice treated with vehicle started to die between 12 h and 24 h after CLP, with a death rate reaching 26.7% and 60.0% after 36 h and 96 h after CLP, respectively. The overall mortality in this group, at the end of the observation period, was 73.3%, and the corresponding area under the curve was 8.117 (arbitrary units). The lethality was markedly delayed in mice treated orally with HE-Bu. Their areas under the curve were increased to 12.420, 12.726, and 12.492 after treating with HE-Bu 75, 150, and 300 mg·kg−1, respectively. At the end of the study, the overall survival in these HE-Bu groups was 25%, 50%, and 57%. No death occurred in the sham-operated mice and its corresponding value for area under the lethality curve was 16.800 (arbitrary units). This result could be attributable to an anti-inflammatory activity, which is consistent with previous literature findings [5].


Analysis of Flavonoids from Eugenia uniflora Leaves and Its Protective Effect against Murine Sepsis.

Rattmann YD, de Souza LM, Malquevicz-Paiva SM, Dartora N, Sassaki GL, Gorin PA, Iacomini M - Evid Based Complement Alternat Med (2012)

HE-Bu obtained from Eugenia uniflora leaves protects against sepsis-induced lethality and inhibits myeloperoxidase activity (measured after 6 h postoperation). Mice (10 animals/group) were orally administered various doses of HE-Bu (75, 150 or 300 mg·kg−1), vehicle (3% ethanol), or dexamethasone (0.5 mg·kg−1 s.c.). MPO graph: values represent means ± SEM. ***P < 0.001, and **P < 0.01, indicated value versus CLP plus vehicle group; ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. Survival graph: survival analyses were compared by a logrank test. These calculations were performed with SigmaStat v3.10 (Systat Software Inc, Richmond, CA, USA). The  hypothesis was rejected when P < 0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3539388&req=5

fig1: HE-Bu obtained from Eugenia uniflora leaves protects against sepsis-induced lethality and inhibits myeloperoxidase activity (measured after 6 h postoperation). Mice (10 animals/group) were orally administered various doses of HE-Bu (75, 150 or 300 mg·kg−1), vehicle (3% ethanol), or dexamethasone (0.5 mg·kg−1 s.c.). MPO graph: values represent means ± SEM. ***P < 0.001, and **P < 0.01, indicated value versus CLP plus vehicle group; ###P < 0.001, CLP plus vehicle versus sham. ANOVA followed by Bonferroni's test. Survival graph: survival analyses were compared by a logrank test. These calculations were performed with SigmaStat v3.10 (Systat Software Inc, Richmond, CA, USA). The hypothesis was rejected when P < 0.05.
Mentions: It was observed that lethality was markedly delayed in mice orally administered with HE-Bu (see Figure 1(a)). Mice treated with vehicle started to die between 12 h and 24 h after CLP, with a death rate reaching 26.7% and 60.0% after 36 h and 96 h after CLP, respectively. The overall mortality in this group, at the end of the observation period, was 73.3%, and the corresponding area under the curve was 8.117 (arbitrary units). The lethality was markedly delayed in mice treated orally with HE-Bu. Their areas under the curve were increased to 12.420, 12.726, and 12.492 after treating with HE-Bu 75, 150, and 300 mg·kg−1, respectively. At the end of the study, the overall survival in these HE-Bu groups was 25%, 50%, and 57%. No death occurred in the sham-operated mice and its corresponding value for area under the lethality curve was 16.800 (arbitrary units). This result could be attributable to an anti-inflammatory activity, which is consistent with previous literature findings [5].

Bottom Line: This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea.The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells.Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, 81531-980 Curitiba, PR, Brazil ; Departamento de Saúde Comunitária, Universidade Federal do Paraná, Rua Padre Camargo, 280, Alto da Glória, 80060-240 Curitiba, PR, Brazil.

ABSTRACT
Eugenia uniflora, referred to as Pitanga cherry shrub, is largely distributed in tropical and subtropical America. This plant is cultivated in many countries and it is suitable for the production of juice, frozen pulp, and tea. Besides, it can be used as treatment for inflammatory diseases. We reported that a flavonoid-rich fraction (HE-Bu) obtained from leaves decreased the lethality induced by cecal ligation and puncture (CLP), a clinically relevant model of sepsis. The oral administration of HE-Bu reduced the late mortality rate by 30%, prevented neutrophil accumulation in lungs, decreased TNF-α and IL-1β serum levels, and markedly decreased iNOS and COX-2 protein expression by ileum cells. Chemical investigation showed myricetin and quercetin rhamnosides as the major components of this fraction. The results showed that HE-Bu protected mice from sepsis and indicated that this edible plant produces compounds that could be considered as potential adjuvants for sepsis treatment.

No MeSH data available.


Related in: MedlinePlus