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Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor.

Ou CW, Sun M, Sadej W, Gibb W - Obstet Gynecol Int (2012)

Bottom Line: Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor.In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues.These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of Ottawa, The Ottawa Hospital, General Campus, 501 Smyth Road, Room 8420, Ottawa, ON, Canada K1H 8L6.

ABSTRACT
Human fetal membranes play an important role in term and preterm labor and are responsive to steroids. We examined the expression of steroid receptor coactivators in fetal membranes obtained prior to and following labor at term. Proteins were localized by immunohistochemistry, Western analysis was carried out in nuclear extracts, and mRNA levels were determined by real-time RT-PCR. SRC-1, SRC-2, p300, and PCAF proteins were present in all nuclear extracts. The amnion nuclei expressed higher levels of SRC-1, p300, and PCAF than nuclei from the chorion-decidua, whereas the reverse was true for SRC-2. Chorion-decidua from patients before labor expressed higher levels of SRC-1 than those from patients after labor. Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor. In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues. These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

No MeSH data available.


Related in: MedlinePlus

Immunohistochemistry of unseparated fetal membranes stained with antisera specific to SRC-1 (a), SRC-2 (b), p300 (c), and PCAF (d). No counterstain was applied. All pictures were taken at 200× magnification. A: amnion epithelium. Ch: chorion laeve. D: decidua.
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fig6: Immunohistochemistry of unseparated fetal membranes stained with antisera specific to SRC-1 (a), SRC-2 (b), p300 (c), and PCAF (d). No counterstain was applied. All pictures were taken at 200× magnification. A: amnion epithelium. Ch: chorion laeve. D: decidua.

Mentions: Coactivators were localized using antibodies specific to SRC-1, SRC-2, p300, and PCAF. SRC-1 was found to be present primarily in the nuclei of the amnion epithelium, chorion laeve, and decidua (Figure 6(a)). In the case of SRC-2, however, it was present in a large amount both in the nuclei and the cytoplasm in the amnion epithelium and chorion laeve. It was also expressed in the nuclei and cytoplasm in decidual cells, but at a lower level (Figure 6(b)). Similar to SRC-1, p300 was also highly concentrated in the nuclei of all layers (Figure 6(c)), whereas PCAF expression was similar to that of SRC-2 and was localized to both the nuclei and cytoplasm in the amnion epithelium, chorion laeve, and decidua (Figure 6(d)). No difference in staining patterns was found in tissues obtained before and after labor.


Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor.

Ou CW, Sun M, Sadej W, Gibb W - Obstet Gynecol Int (2012)

Immunohistochemistry of unseparated fetal membranes stained with antisera specific to SRC-1 (a), SRC-2 (b), p300 (c), and PCAF (d). No counterstain was applied. All pictures were taken at 200× magnification. A: amnion epithelium. Ch: chorion laeve. D: decidua.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3539340&req=5

fig6: Immunohistochemistry of unseparated fetal membranes stained with antisera specific to SRC-1 (a), SRC-2 (b), p300 (c), and PCAF (d). No counterstain was applied. All pictures were taken at 200× magnification. A: amnion epithelium. Ch: chorion laeve. D: decidua.
Mentions: Coactivators were localized using antibodies specific to SRC-1, SRC-2, p300, and PCAF. SRC-1 was found to be present primarily in the nuclei of the amnion epithelium, chorion laeve, and decidua (Figure 6(a)). In the case of SRC-2, however, it was present in a large amount both in the nuclei and the cytoplasm in the amnion epithelium and chorion laeve. It was also expressed in the nuclei and cytoplasm in decidual cells, but at a lower level (Figure 6(b)). Similar to SRC-1, p300 was also highly concentrated in the nuclei of all layers (Figure 6(c)), whereas PCAF expression was similar to that of SRC-2 and was localized to both the nuclei and cytoplasm in the amnion epithelium, chorion laeve, and decidua (Figure 6(d)). No difference in staining patterns was found in tissues obtained before and after labor.

Bottom Line: Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor.In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues.These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of Ottawa, The Ottawa Hospital, General Campus, 501 Smyth Road, Room 8420, Ottawa, ON, Canada K1H 8L6.

ABSTRACT
Human fetal membranes play an important role in term and preterm labor and are responsive to steroids. We examined the expression of steroid receptor coactivators in fetal membranes obtained prior to and following labor at term. Proteins were localized by immunohistochemistry, Western analysis was carried out in nuclear extracts, and mRNA levels were determined by real-time RT-PCR. SRC-1, SRC-2, p300, and PCAF proteins were present in all nuclear extracts. The amnion nuclei expressed higher levels of SRC-1, p300, and PCAF than nuclei from the chorion-decidua, whereas the reverse was true for SRC-2. Chorion-decidua from patients before labor expressed higher levels of SRC-1 than those from patients after labor. Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor. In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues. These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

No MeSH data available.


Related in: MedlinePlus