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Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor.

Ou CW, Sun M, Sadej W, Gibb W - Obstet Gynecol Int (2012)

Bottom Line: Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor.In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues.These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of Ottawa, The Ottawa Hospital, General Campus, 501 Smyth Road, Room 8420, Ottawa, ON, Canada K1H 8L6.

ABSTRACT
Human fetal membranes play an important role in term and preterm labor and are responsive to steroids. We examined the expression of steroid receptor coactivators in fetal membranes obtained prior to and following labor at term. Proteins were localized by immunohistochemistry, Western analysis was carried out in nuclear extracts, and mRNA levels were determined by real-time RT-PCR. SRC-1, SRC-2, p300, and PCAF proteins were present in all nuclear extracts. The amnion nuclei expressed higher levels of SRC-1, p300, and PCAF than nuclei from the chorion-decidua, whereas the reverse was true for SRC-2. Chorion-decidua from patients before labor expressed higher levels of SRC-1 than those from patients after labor. Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor. In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues. These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

No MeSH data available.


Related in: MedlinePlus

Expression of p300 protein (a) and mRNA (b) in the amnion and chorion-decidua (Ch-d) from non-labor group (C/S) and labor group (L). Data were presented as the mean ± SEM. (a) **P < 0.05 between amnion and chorion-decidua. (b) **P < 0.05 between amnion and chorion-decidua. *P < 0.05 between C/S group and labor group within the amnion. Ponceau S staining was used as the internal control for Westerns and GAPDH mRNA as the internal control for mRNA.
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fig4: Expression of p300 protein (a) and mRNA (b) in the amnion and chorion-decidua (Ch-d) from non-labor group (C/S) and labor group (L). Data were presented as the mean ± SEM. (a) **P < 0.05 between amnion and chorion-decidua. (b) **P < 0.05 between amnion and chorion-decidua. *P < 0.05 between C/S group and labor group within the amnion. Ponceau S staining was used as the internal control for Westerns and GAPDH mRNA as the internal control for mRNA.

Mentions: The amnion expressed higher levels of p300 protein than the chorion-decidua. Comparisons within the tissues failed to demonstrate a difference between the tissues obtained before and after labor (Figure 4(a)). In contrast, p300 mRNA level was higher in the chorion-decidua than in the amnion. However, the level was higher in the labor group compared to the non-labor group only in the amnion but not in the chorion-decidua (Figure 4(b)).


Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor.

Ou CW, Sun M, Sadej W, Gibb W - Obstet Gynecol Int (2012)

Expression of p300 protein (a) and mRNA (b) in the amnion and chorion-decidua (Ch-d) from non-labor group (C/S) and labor group (L). Data were presented as the mean ± SEM. (a) **P < 0.05 between amnion and chorion-decidua. (b) **P < 0.05 between amnion and chorion-decidua. *P < 0.05 between C/S group and labor group within the amnion. Ponceau S staining was used as the internal control for Westerns and GAPDH mRNA as the internal control for mRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig4: Expression of p300 protein (a) and mRNA (b) in the amnion and chorion-decidua (Ch-d) from non-labor group (C/S) and labor group (L). Data were presented as the mean ± SEM. (a) **P < 0.05 between amnion and chorion-decidua. (b) **P < 0.05 between amnion and chorion-decidua. *P < 0.05 between C/S group and labor group within the amnion. Ponceau S staining was used as the internal control for Westerns and GAPDH mRNA as the internal control for mRNA.
Mentions: The amnion expressed higher levels of p300 protein than the chorion-decidua. Comparisons within the tissues failed to demonstrate a difference between the tissues obtained before and after labor (Figure 4(a)). In contrast, p300 mRNA level was higher in the chorion-decidua than in the amnion. However, the level was higher in the labor group compared to the non-labor group only in the amnion but not in the chorion-decidua (Figure 4(b)).

Bottom Line: Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor.In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues.These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of Ottawa, The Ottawa Hospital, General Campus, 501 Smyth Road, Room 8420, Ottawa, ON, Canada K1H 8L6.

ABSTRACT
Human fetal membranes play an important role in term and preterm labor and are responsive to steroids. We examined the expression of steroid receptor coactivators in fetal membranes obtained prior to and following labor at term. Proteins were localized by immunohistochemistry, Western analysis was carried out in nuclear extracts, and mRNA levels were determined by real-time RT-PCR. SRC-1, SRC-2, p300, and PCAF proteins were present in all nuclear extracts. The amnion nuclei expressed higher levels of SRC-1, p300, and PCAF than nuclei from the chorion-decidua, whereas the reverse was true for SRC-2. Chorion-decidua from patients before labor expressed higher levels of SRC-1 than those from patients after labor. Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor. In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues. These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

No MeSH data available.


Related in: MedlinePlus