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Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor.

Ou CW, Sun M, Sadej W, Gibb W - Obstet Gynecol Int (2012)

Bottom Line: Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor.In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues.These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of Ottawa, The Ottawa Hospital, General Campus, 501 Smyth Road, Room 8420, Ottawa, ON, Canada K1H 8L6.

ABSTRACT
Human fetal membranes play an important role in term and preterm labor and are responsive to steroids. We examined the expression of steroid receptor coactivators in fetal membranes obtained prior to and following labor at term. Proteins were localized by immunohistochemistry, Western analysis was carried out in nuclear extracts, and mRNA levels were determined by real-time RT-PCR. SRC-1, SRC-2, p300, and PCAF proteins were present in all nuclear extracts. The amnion nuclei expressed higher levels of SRC-1, p300, and PCAF than nuclei from the chorion-decidua, whereas the reverse was true for SRC-2. Chorion-decidua from patients before labor expressed higher levels of SRC-1 than those from patients after labor. Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor. In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues. These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

No MeSH data available.


Related in: MedlinePlus

Representative Western blots of amnion and chorion-decidua (Ch-d) of six different tissues from non-labor patients undergoing elective cesarean section (C/S) and six labor patients after spontaneous vaginal delivery. Blots were probed with antisera specific to SRC-1, SRC-2, p300, and PCAF. Nuclear extracts from K562 cells (Santa Cruz) were used as a positive control for these proteins (+). Lanes marked 1–3 represent C/S tissues and 4–6 represent labor tissues. Typical Ponceau S staining, used as loading control, is shown in the lower panel.
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fig1: Representative Western blots of amnion and chorion-decidua (Ch-d) of six different tissues from non-labor patients undergoing elective cesarean section (C/S) and six labor patients after spontaneous vaginal delivery. Blots were probed with antisera specific to SRC-1, SRC-2, p300, and PCAF. Nuclear extracts from K562 cells (Santa Cruz) were used as a positive control for these proteins (+). Lanes marked 1–3 represent C/S tissues and 4–6 represent labor tissues. Typical Ponceau S staining, used as loading control, is shown in the lower panel.

Mentions: Western analysis of nuclear proteins from human fetal membranes showed that SRC-1, SRC-2, p300, and PCAF were expressed in the nuclear extracts from the amnion and chorion-decidua (Figure 1). However, SRC-3, CBP, Brg-1, and Brm were not detectable.


Expression of Nuclear Receptor Coactivators in the Human Fetal Membranes at Term before and after Labor.

Ou CW, Sun M, Sadej W, Gibb W - Obstet Gynecol Int (2012)

Representative Western blots of amnion and chorion-decidua (Ch-d) of six different tissues from non-labor patients undergoing elective cesarean section (C/S) and six labor patients after spontaneous vaginal delivery. Blots were probed with antisera specific to SRC-1, SRC-2, p300, and PCAF. Nuclear extracts from K562 cells (Santa Cruz) were used as a positive control for these proteins (+). Lanes marked 1–3 represent C/S tissues and 4–6 represent labor tissues. Typical Ponceau S staining, used as loading control, is shown in the lower panel.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3539340&req=5

fig1: Representative Western blots of amnion and chorion-decidua (Ch-d) of six different tissues from non-labor patients undergoing elective cesarean section (C/S) and six labor patients after spontaneous vaginal delivery. Blots were probed with antisera specific to SRC-1, SRC-2, p300, and PCAF. Nuclear extracts from K562 cells (Santa Cruz) were used as a positive control for these proteins (+). Lanes marked 1–3 represent C/S tissues and 4–6 represent labor tissues. Typical Ponceau S staining, used as loading control, is shown in the lower panel.
Mentions: Western analysis of nuclear proteins from human fetal membranes showed that SRC-1, SRC-2, p300, and PCAF were expressed in the nuclear extracts from the amnion and chorion-decidua (Figure 1). However, SRC-3, CBP, Brg-1, and Brm were not detectable.

Bottom Line: Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor.In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues.These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

View Article: PubMed Central - PubMed

Affiliation: Department of Obstetrics and Gynecology, University of Ottawa, The Ottawa Hospital, General Campus, 501 Smyth Road, Room 8420, Ottawa, ON, Canada K1H 8L6.

ABSTRACT
Human fetal membranes play an important role in term and preterm labor and are responsive to steroids. We examined the expression of steroid receptor coactivators in fetal membranes obtained prior to and following labor at term. Proteins were localized by immunohistochemistry, Western analysis was carried out in nuclear extracts, and mRNA levels were determined by real-time RT-PCR. SRC-1, SRC-2, p300, and PCAF proteins were present in all nuclear extracts. The amnion nuclei expressed higher levels of SRC-1, p300, and PCAF than nuclei from the chorion-decidua, whereas the reverse was true for SRC-2. Chorion-decidua from patients before labor expressed higher levels of SRC-1 than those from patients after labor. Also, the PCAF level was higher in the amnion obtained before labor than the same tissue obtained after labor. In contrast to the protein expression, mRNA levels of SRC-1 and p300 were higher in the chorion-decidua compared to the amnion, whereas there was no difference in levels of SRC-2 and PCAF mRNAs between these two tissues. These data underline that the regulation of the expression of the coactivators in these tissues occurs during labor and is complex and tissue specific.

No MeSH data available.


Related in: MedlinePlus