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Westerdykella reniformis sp. nov., producing the antibiotic metabolites melinacidin IV and chetracin B.

Ebead GA, Overy DP, Berrué F, Kerr RG - IMA Fungus (2012)

Bottom Line: Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences.Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove.Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada, C1A 4P3;

ABSTRACT
Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences. Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove. The isolate was obtained from a red alga (Polysiphonia sp.) collected from the tidal zone in Canada at low tide. Organic extracts enriched in extrolites, obtained from fermentation on a rice-based media, inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), S. warneri, and Proteus vulgaris. Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

No MeSH data available.


Related in: MedlinePlus

Chemical structures of biologically active secondary metabolites produced by Westerdykella species.
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Figure 4: Chemical structures of biologically active secondary metabolites produced by Westerdykella species.

Mentions: Two successive orthogonal fractionations (reverse phase then normal phase) of the MeOH:H2O extract obtained after liquid-liquid partitions yielded three fractions (3–5) exhibiting strong antimicrobial activities. Chemical profiling by LC-HRMS coupled to a universal detector (ELSD) suggested that two major compounds were responsible for the observed biological activities (Fig. 3). The interpretation of the HRMS data indicated the molecular formulae C30H28N6O8S4 (m/z 729.09076 [M+H]+, Δ -2.2 ppm) and C30H28N6O8S5 (m/z 761.06274 [M+H]+, Δ -2.3 ppm) respectively and was in agreement with the observed isotopic pattern and the presence of sulfur atoms. After searches in databases Antibases and SciFinder, the two prominent components with antimicrobial properties were identified as the known metabolites melinacidin IV and chetracin B (Fig. 4) which belong to the important class of biologically active metabolites: epipolythiodioxopiperazines (ETPs) (Argoudelis & Mizsak 1977, Li et al. 2012). This conclusion was further confirmed by 1H NMR analysis after the purification of both metabolites by normal phase HPLC.


Westerdykella reniformis sp. nov., producing the antibiotic metabolites melinacidin IV and chetracin B.

Ebead GA, Overy DP, Berrué F, Kerr RG - IMA Fungus (2012)

Chemical structures of biologically active secondary metabolites produced by Westerdykella species.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539322&req=5

Figure 4: Chemical structures of biologically active secondary metabolites produced by Westerdykella species.
Mentions: Two successive orthogonal fractionations (reverse phase then normal phase) of the MeOH:H2O extract obtained after liquid-liquid partitions yielded three fractions (3–5) exhibiting strong antimicrobial activities. Chemical profiling by LC-HRMS coupled to a universal detector (ELSD) suggested that two major compounds were responsible for the observed biological activities (Fig. 3). The interpretation of the HRMS data indicated the molecular formulae C30H28N6O8S4 (m/z 729.09076 [M+H]+, Δ -2.2 ppm) and C30H28N6O8S5 (m/z 761.06274 [M+H]+, Δ -2.3 ppm) respectively and was in agreement with the observed isotopic pattern and the presence of sulfur atoms. After searches in databases Antibases and SciFinder, the two prominent components with antimicrobial properties were identified as the known metabolites melinacidin IV and chetracin B (Fig. 4) which belong to the important class of biologically active metabolites: epipolythiodioxopiperazines (ETPs) (Argoudelis & Mizsak 1977, Li et al. 2012). This conclusion was further confirmed by 1H NMR analysis after the purification of both metabolites by normal phase HPLC.

Bottom Line: Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences.Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove.Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada, C1A 4P3;

ABSTRACT
Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences. Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove. The isolate was obtained from a red alga (Polysiphonia sp.) collected from the tidal zone in Canada at low tide. Organic extracts enriched in extrolites, obtained from fermentation on a rice-based media, inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), S. warneri, and Proteus vulgaris. Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

No MeSH data available.


Related in: MedlinePlus