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Westerdykella reniformis sp. nov., producing the antibiotic metabolites melinacidin IV and chetracin B.

Ebead GA, Overy DP, Berrué F, Kerr RG - IMA Fungus (2012)

Bottom Line: Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences.Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove.Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada, C1A 4P3;

ABSTRACT
Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences. Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove. The isolate was obtained from a red alga (Polysiphonia sp.) collected from the tidal zone in Canada at low tide. Organic extracts enriched in extrolites, obtained from fermentation on a rice-based media, inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), S. warneri, and Proteus vulgaris. Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

No MeSH data available.


Related in: MedlinePlus

Bootstrap consensus tree inferred from 2000 replicates using the neighbor-joining method based on ITS rDNA sequences. The percentage of replicate trees (> 50 %) in which the associated taxa clustered together in the bootstrap tests of 2000 replicates are shown next to the branches. Evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. The tree was rooted with Verruculina enalia (CBS 304.66).
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Figure 1: Bootstrap consensus tree inferred from 2000 replicates using the neighbor-joining method based on ITS rDNA sequences. The percentage of replicate trees (> 50 %) in which the associated taxa clustered together in the bootstrap tests of 2000 replicates are shown next to the branches. Evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. The tree was rooted with Verruculina enalia (CBS 304.66).

Mentions: The ITS rDNA gene was analysed to determine the relative evolutionary history of isolate RKGE 35 with multiple isolates of other representative Westerdykella spp. The evolutionary history was inferred by the bootstrap consensus tree (Fig. 1) constructed using the neighbour-joining method and 2000 bootstrap replicates. The analysis involved 39 sequences and included 409 nucleotide positions in the final dataset with an overall mean distance calculated as 0.137 with a standard error of 0.013. The genus Westerdykella formed a well-supported monophyletic clade distinct from other members of Sporormiaceae. Within the Westerdykella clade, sequences from individual isolates formed distinct species groups with high bootstrap support; however, evolutionary relatedness of species within the genus was difficult to infer due to separation with low associated bootstrap values. Isolate RKGE 35 formed a sister clade to that of W. cylindrica, represented by isolate CBS 454.72 (= ATCC 24077). Isolate CBS 604.97, representing the unpublished species W. rapa-nuiensis ined., clustered together within the W. dispersa clade along with the W. multispora isolate CBS 391.51.


Westerdykella reniformis sp. nov., producing the antibiotic metabolites melinacidin IV and chetracin B.

Ebead GA, Overy DP, Berrué F, Kerr RG - IMA Fungus (2012)

Bootstrap consensus tree inferred from 2000 replicates using the neighbor-joining method based on ITS rDNA sequences. The percentage of replicate trees (> 50 %) in which the associated taxa clustered together in the bootstrap tests of 2000 replicates are shown next to the branches. Evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. The tree was rooted with Verruculina enalia (CBS 304.66).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539322&req=5

Figure 1: Bootstrap consensus tree inferred from 2000 replicates using the neighbor-joining method based on ITS rDNA sequences. The percentage of replicate trees (> 50 %) in which the associated taxa clustered together in the bootstrap tests of 2000 replicates are shown next to the branches. Evolutionary distances were computed using the maximum composite likelihood method and are in the units of the number of base substitutions per site. The tree was rooted with Verruculina enalia (CBS 304.66).
Mentions: The ITS rDNA gene was analysed to determine the relative evolutionary history of isolate RKGE 35 with multiple isolates of other representative Westerdykella spp. The evolutionary history was inferred by the bootstrap consensus tree (Fig. 1) constructed using the neighbour-joining method and 2000 bootstrap replicates. The analysis involved 39 sequences and included 409 nucleotide positions in the final dataset with an overall mean distance calculated as 0.137 with a standard error of 0.013. The genus Westerdykella formed a well-supported monophyletic clade distinct from other members of Sporormiaceae. Within the Westerdykella clade, sequences from individual isolates formed distinct species groups with high bootstrap support; however, evolutionary relatedness of species within the genus was difficult to infer due to separation with low associated bootstrap values. Isolate RKGE 35 formed a sister clade to that of W. cylindrica, represented by isolate CBS 454.72 (= ATCC 24077). Isolate CBS 604.97, representing the unpublished species W. rapa-nuiensis ined., clustered together within the W. dispersa clade along with the W. multispora isolate CBS 391.51.

Bottom Line: Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences.Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove.Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Ave., Charlottetown, PEI, Canada, C1A 4P3;

ABSTRACT
Westerdykella reniformis Ebead & Overy sp. nov. is described based on morphology and phylogenetic analyses using ITS, nLSU rDNA, and β-tubulin gene sequences. Westerdykella reniformis is characterized by the production of cleistothecioid ascomata, containing small globose to subglobose asci with 32, aseptate, dark colored, pronouncedly reniform ascospores having a concave central groove. The isolate was obtained from a red alga (Polysiphonia sp.) collected from the tidal zone in Canada at low tide. Organic extracts enriched in extrolites, obtained from fermentation on a rice-based media, inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), S. warneri, and Proteus vulgaris. Presented here is the identification of the compounds responsible for the observed antimicrobial activity, the taxonomic description of W. reniformis, and a dichotomous key to the known species of Westerdykella based on macro- and micromorphological characters.

No MeSH data available.


Related in: MedlinePlus