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Two novel species of Aspergillus section Nigri from indoor air.

Jurjević Z, Peterson SW, Stea G, Solfrizzo M, Varga J, Hubka V, Perrone G - IMA Fungus (2012)

Bottom Line: Aspergillus brunneoviolaceus (syn.A. fijiensis) and A. uvarum are reported for the first time from the USA and from the indoor air environment.The newly described species do not produce ochratoxin A.

View Article: PubMed Central - PubMed

Affiliation: EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, New Jersey 08077 USA;

ABSTRACT
Aspergillus floridensis and A. trinidadensis spp. nov. are described as novel uniseriate species of Aspergillus section Nigri isolated from air samples. To describe the species we used phenotypes from 7-d Czapek yeast extract agar culture (CYA), creatine agar culture (CREA) and malt extract agar culture (MEA), with support by molecular analysis of the β-tubulin, calmodulin, RNA polymerase II (RPB2), and translation elongation factor-alpha (TEF) gene amplified and sequenced from 56 air isolates and one isolate from almonds belonging to Aspergillus sectionNigri.Aspergillus floridensis is closely related to A. aculeatus, and A. trinidadensis is closely related to A. aculeatinus. Aspergillus brunneoviolaceus (syn. A. fijiensis) and A. uvarum are reported for the first time from the USA and from the indoor air environment. The newly described species do not produce ochratoxin A.

No MeSH data available.


Phylogenetic trees produced from the combined sequence data of four loci (CaM, benA, RPB2 and TEF) of 57 strains of uniseriate black Aspergillus, 28 reference strains of species belonging to Aspergillus section Nigri, and A. flavus (ITEM 7526) as outgroup. Numbers above branches are bootstrap values. Only values above 70 % are indicated. The evolutionary history was inferred using the Neighbour-Joining method computed with the Maximum Likelihood Evolutionary method.
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Figure 1: Phylogenetic trees produced from the combined sequence data of four loci (CaM, benA, RPB2 and TEF) of 57 strains of uniseriate black Aspergillus, 28 reference strains of species belonging to Aspergillus section Nigri, and A. flavus (ITEM 7526) as outgroup. Numbers above branches are bootstrap values. Only values above 70 % are indicated. The evolutionary history was inferred using the Neighbour-Joining method computed with the Maximum Likelihood Evolutionary method.

Mentions: The multilocus analysis was performed on 56 isolates collected from air (52 homes and 4 outside samples) from 17 states of the United States, Bermuda, Martinique, Trinidad and Tobago, and one isolated from almonds in the Czech Republic (Table 1), along with 28 reference and ex-type strains from Aspergillus section Nigri (Table 4). The ex-type strain of Aspergillus flavus (ITEM 7526) was used as outgroup. The percentage of variable sites and parsimony informative sites for each locus differ, the benA sequences have the highest percentage of variable and parsimony informative sites, the CaM sequences have the highest nucleotide diversity, TEF sequences have the lowest variability and RPB2 has lower sequence diversity than CaM and benA but the highest number of informative sites (Table 5). After a preliminary analysis using MEGA5 Neighbour-Joining, the best substitution model among the evolutionary models in MEGA5 was calculated. The best model was Tamura-Nei with Gamma distribution (TN93 + G). Evolutionary history was inferred using the Neighbor-Joining method. The tree with the highest log likelihood is shown (Fig 1). Bootstrap proportions are shown next to the branches. The tree is drawn to scale, with branch lengths reflecting evolutionary distance computed using the Maximum Composite Likelihood method as number of base substitutions per site (MEGA5). The rate variation among sites was modeled with a gamma distribution (shape parameter = 0.3). Phylogenetic analysis was conducted first on the four single locus alignments and subsequently the combined alignment of the four loci. The single locus and four locus combined data trees contained the same topology fulfilling the requirements of genealogical concordance phylogenetic species recognition (GCPSR, Taylor et al. 2000).


Two novel species of Aspergillus section Nigri from indoor air.

Jurjević Z, Peterson SW, Stea G, Solfrizzo M, Varga J, Hubka V, Perrone G - IMA Fungus (2012)

Phylogenetic trees produced from the combined sequence data of four loci (CaM, benA, RPB2 and TEF) of 57 strains of uniseriate black Aspergillus, 28 reference strains of species belonging to Aspergillus section Nigri, and A. flavus (ITEM 7526) as outgroup. Numbers above branches are bootstrap values. Only values above 70 % are indicated. The evolutionary history was inferred using the Neighbour-Joining method computed with the Maximum Likelihood Evolutionary method.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539319&req=5

Figure 1: Phylogenetic trees produced from the combined sequence data of four loci (CaM, benA, RPB2 and TEF) of 57 strains of uniseriate black Aspergillus, 28 reference strains of species belonging to Aspergillus section Nigri, and A. flavus (ITEM 7526) as outgroup. Numbers above branches are bootstrap values. Only values above 70 % are indicated. The evolutionary history was inferred using the Neighbour-Joining method computed with the Maximum Likelihood Evolutionary method.
Mentions: The multilocus analysis was performed on 56 isolates collected from air (52 homes and 4 outside samples) from 17 states of the United States, Bermuda, Martinique, Trinidad and Tobago, and one isolated from almonds in the Czech Republic (Table 1), along with 28 reference and ex-type strains from Aspergillus section Nigri (Table 4). The ex-type strain of Aspergillus flavus (ITEM 7526) was used as outgroup. The percentage of variable sites and parsimony informative sites for each locus differ, the benA sequences have the highest percentage of variable and parsimony informative sites, the CaM sequences have the highest nucleotide diversity, TEF sequences have the lowest variability and RPB2 has lower sequence diversity than CaM and benA but the highest number of informative sites (Table 5). After a preliminary analysis using MEGA5 Neighbour-Joining, the best substitution model among the evolutionary models in MEGA5 was calculated. The best model was Tamura-Nei with Gamma distribution (TN93 + G). Evolutionary history was inferred using the Neighbor-Joining method. The tree with the highest log likelihood is shown (Fig 1). Bootstrap proportions are shown next to the branches. The tree is drawn to scale, with branch lengths reflecting evolutionary distance computed using the Maximum Composite Likelihood method as number of base substitutions per site (MEGA5). The rate variation among sites was modeled with a gamma distribution (shape parameter = 0.3). Phylogenetic analysis was conducted first on the four single locus alignments and subsequently the combined alignment of the four loci. The single locus and four locus combined data trees contained the same topology fulfilling the requirements of genealogical concordance phylogenetic species recognition (GCPSR, Taylor et al. 2000).

Bottom Line: Aspergillus brunneoviolaceus (syn.A. fijiensis) and A. uvarum are reported for the first time from the USA and from the indoor air environment.The newly described species do not produce ochratoxin A.

View Article: PubMed Central - PubMed

Affiliation: EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, New Jersey 08077 USA;

ABSTRACT
Aspergillus floridensis and A. trinidadensis spp. nov. are described as novel uniseriate species of Aspergillus section Nigri isolated from air samples. To describe the species we used phenotypes from 7-d Czapek yeast extract agar culture (CYA), creatine agar culture (CREA) and malt extract agar culture (MEA), with support by molecular analysis of the β-tubulin, calmodulin, RNA polymerase II (RPB2), and translation elongation factor-alpha (TEF) gene amplified and sequenced from 56 air isolates and one isolate from almonds belonging to Aspergillus sectionNigri.Aspergillus floridensis is closely related to A. aculeatus, and A. trinidadensis is closely related to A. aculeatinus. Aspergillus brunneoviolaceus (syn. A. fijiensis) and A. uvarum are reported for the first time from the USA and from the indoor air environment. The newly described species do not produce ochratoxin A.

No MeSH data available.