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A new Leucoagaricus species of section Piloselli (Agaricales, Agaricaceae) from Spain.

Muñoz G, Caballero A, Contu M, Vizzini A - IMA Fungus (2012)

Bottom Line: The new species Leucoagaricus variicolor is described from a public park in Zaragoza, Spain, based on both morphological and molecular characters.Illustrations of fresh basidiomata in situ and of the main macro- and micromorphological features are added.Leucoagaricusvariicolor belongs to section Piloselli and is compared with similar taxa.

View Article: PubMed Central - PubMed

Affiliation: Avda. Valvanera 32, 5.° dcha. 26500 Calahorra, La Rioja, Spain.

ABSTRACT
The new species Leucoagaricus variicolor is described from a public park in Zaragoza, Spain, based on both morphological and molecular characters. Illustrations of fresh basidiomata in situ and of the main macro- and micromorphological features are added. Leucoagaricusvariicolor belongs to section Piloselli and is compared with similar taxa.

No MeSH data available.


Related in: MedlinePlus

Maximum Likelihood phylogram obtained from the ITS (ITS1-5.8S-ITS2) sequence alignment of Leucoagaricus spp. Cystolepiota seminuda was used as outgroup taxon. MLB values over 50 % are given above branches. Newly sequenced collections are in bold.
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Figure 1: Maximum Likelihood phylogram obtained from the ITS (ITS1-5.8S-ITS2) sequence alignment of Leucoagaricus spp. Cystolepiota seminuda was used as outgroup taxon. MLB values over 50 % are given above branches. Newly sequenced collections are in bold.

Mentions: Genomic DNA was isolated from 1 mg of a dried herbarium specimen from four collections (AH-40328, GM-2454, GM-2485, and GM-2486), using the DNeasy Plant Mini Kit (Qiagen, Milan) according to the manufacturer’s instructions. Universal primers ITS1F/ITS4 were used for the ITS region amplification (White et al. 1990, Gardes & Bruns 1993). Amplification reactions were performed in a PE9700 thermal cycler (Perkin-Elmer, Applied Biosystems) following Vizzini et al. (2011). The PCR products were purified with the AMPure XP kit (Beckman) and sequenced by MACROGEN (Seoul, Republic of Korea). The sequences were submitted to GenBank (http://www.ncbi.nlm.nih.gov/genbank/) and their accession numbers are reported in Fig. 1.


A new Leucoagaricus species of section Piloselli (Agaricales, Agaricaceae) from Spain.

Muñoz G, Caballero A, Contu M, Vizzini A - IMA Fungus (2012)

Maximum Likelihood phylogram obtained from the ITS (ITS1-5.8S-ITS2) sequence alignment of Leucoagaricus spp. Cystolepiota seminuda was used as outgroup taxon. MLB values over 50 % are given above branches. Newly sequenced collections are in bold.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539314&req=5

Figure 1: Maximum Likelihood phylogram obtained from the ITS (ITS1-5.8S-ITS2) sequence alignment of Leucoagaricus spp. Cystolepiota seminuda was used as outgroup taxon. MLB values over 50 % are given above branches. Newly sequenced collections are in bold.
Mentions: Genomic DNA was isolated from 1 mg of a dried herbarium specimen from four collections (AH-40328, GM-2454, GM-2485, and GM-2486), using the DNeasy Plant Mini Kit (Qiagen, Milan) according to the manufacturer’s instructions. Universal primers ITS1F/ITS4 were used for the ITS region amplification (White et al. 1990, Gardes & Bruns 1993). Amplification reactions were performed in a PE9700 thermal cycler (Perkin-Elmer, Applied Biosystems) following Vizzini et al. (2011). The PCR products were purified with the AMPure XP kit (Beckman) and sequenced by MACROGEN (Seoul, Republic of Korea). The sequences were submitted to GenBank (http://www.ncbi.nlm.nih.gov/genbank/) and their accession numbers are reported in Fig. 1.

Bottom Line: The new species Leucoagaricus variicolor is described from a public park in Zaragoza, Spain, based on both morphological and molecular characters.Illustrations of fresh basidiomata in situ and of the main macro- and micromorphological features are added.Leucoagaricusvariicolor belongs to section Piloselli and is compared with similar taxa.

View Article: PubMed Central - PubMed

Affiliation: Avda. Valvanera 32, 5.° dcha. 26500 Calahorra, La Rioja, Spain.

ABSTRACT
The new species Leucoagaricus variicolor is described from a public park in Zaragoza, Spain, based on both morphological and molecular characters. Illustrations of fresh basidiomata in situ and of the main macro- and micromorphological features are added. Leucoagaricusvariicolor belongs to section Piloselli and is compared with similar taxa.

No MeSH data available.


Related in: MedlinePlus