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Development of merosporangia in Linderina pennispora (Kickxellales, Kickxellaceae).

Zain ME, Moss ST, El-Sheikh HH - IMA Fungus (2012)

Bottom Line: The sporangiophore was erect, cylindrical, and narrower (4-8 μm) than the vegetative mycelium (8-12 μm).The pseudophialides had a round, ca. 1.5 μm diam, base with a narrower, 0.7-0.8 μm diam lobed, cylindrical neck structure in the distal region which extended to the pseudophialide neck.Detached merosporangia had a single, acicular, unbranched, 3-5 μm long, ca. 0.1 μm diam "appendage" that was attached to the merosporangiospore inner cell wall layer and passed through the septum plug to the pseudophialide.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi Arabia; ; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.

ABSTRACT
The vegetative and sporulating structures of Linderina pennispora are described using scanning and transmission electron microscopy. The vegetative hyphae and sporangiophores were regularly septate, possessed a two-layered wall, and coated with rod-shaped, 0.2-0.7 μm long, 0.15-0.25 μm wide ornamentations. The sporangiophore was erect, cylindrical, and narrower (4-8 μm) than the vegetative mycelium (8-12 μm). The mature sporocladium was ovoid to dome-shaped, sessile, non-septate, 18-24 μm diam, possessed a two-layered wall, and coated with rod-shaped ornamentations. Mature pseudophialides were ellipsoid, 2.0-2.5 μm wide, 4-7 μm long, possessed a two-layered wall, and formed in a series of concentric groups radiating from the "apex" of the sporocladium. The pseudophialides had a round, ca. 1.5 μm diam, base with a narrower, 0.7-0.8 μm diam lobed, cylindrical neck structure in the distal region which extended to the pseudophialide neck. The merosporangia were obovate, 3-4 μm wide near the base, and narrowed distally to 2.0-2.5 μm wide, 18-23 μm long, possessed a three-layered wall, with regular surface annulation with interconnecting ridges, but lacked rod-shaped ornamentations. The merosporangia contained a single, obovate, 2.3-2.5 μm diam merosporangiospore, with a ca. 1 μm diam papilla-like base, that possessed a four-layered wall. Detached merosporangia had a single, acicular, unbranched, 3-5 μm long, ca. 0.1 μm diam "appendage" that was attached to the merosporangiospore inner cell wall layer and passed through the septum plug to the pseudophialide.

No MeSH data available.


Related in: MedlinePlus

Scanning electron (A, B, D, F–I) and transmission micrographs (C, E, J, K) of sporulating structures of Linderina pennispora. A. Immature, globose sporocladia formed apically on the sporangiophore (Sp). Bars = 5 μm. B. Pseudophialide initials formed on sporocladia (S). Bars = 5 μm. C. Longitudinal sections of a sporocladium (S) with pseudophialides initials. Bar = 1 μm. D. Ellipsoid pseudophialides (Ps) with merosporangium initials. S = Sporocladium, Sp = Sorangiophores. Bar = 5 μm. E. Longitudinal sections through the distal region of the pseudophialide (Ps), the septum between a pseudophialide neck (PsN) and merosporangium (M). The pore in the septal cross wall (CW) contains a biconvex septal-plug (P), and the base of the merosporangiospore (Ms) is constricted towards the septum. P = septal-plug. Bar = 0.25 μm (c). F. Mature sporocladium (S), pseudophialides (Ps) and merosporangia (M). Bar = 5 μm. G. Obovate merosporangia (M) on pseudophialides (Ps). Note the regular annulations on the merosporangia surface, and the septum on the pseudophialide neck (N). Bar = 5 μm. H. Merosporangium and merosporangiospore cell wall. Three wall-layered of merosporangium (MW) and four-layered wall of the merosporangiospore (MsW). Bar = 0.1 μm. I. Released merosporangia (M) with a single, basally-attached “appendage”. Bar = 2 μm. J. Ellipsoid pseudophialides (Ps) coated with rod-shape ornamentations. Bar = 2 μm. K. Base of merosporangiospore (MB) with appendage (A) attached to the inner layer of the merosporangiospore cell wall and passing through the septum pore to the pseudophialide neck. Bar = 0.1 μm.
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Figure 1: Scanning electron (A, B, D, F–I) and transmission micrographs (C, E, J, K) of sporulating structures of Linderina pennispora. A. Immature, globose sporocladia formed apically on the sporangiophore (Sp). Bars = 5 μm. B. Pseudophialide initials formed on sporocladia (S). Bars = 5 μm. C. Longitudinal sections of a sporocladium (S) with pseudophialides initials. Bar = 1 μm. D. Ellipsoid pseudophialides (Ps) with merosporangium initials. S = Sporocladium, Sp = Sorangiophores. Bar = 5 μm. E. Longitudinal sections through the distal region of the pseudophialide (Ps), the septum between a pseudophialide neck (PsN) and merosporangium (M). The pore in the septal cross wall (CW) contains a biconvex septal-plug (P), and the base of the merosporangiospore (Ms) is constricted towards the septum. P = septal-plug. Bar = 0.25 μm (c). F. Mature sporocladium (S), pseudophialides (Ps) and merosporangia (M). Bar = 5 μm. G. Obovate merosporangia (M) on pseudophialides (Ps). Note the regular annulations on the merosporangia surface, and the septum on the pseudophialide neck (N). Bar = 5 μm. H. Merosporangium and merosporangiospore cell wall. Three wall-layered of merosporangium (MW) and four-layered wall of the merosporangiospore (MsW). Bar = 0.1 μm. I. Released merosporangia (M) with a single, basally-attached “appendage”. Bar = 2 μm. J. Ellipsoid pseudophialides (Ps) coated with rod-shape ornamentations. Bar = 2 μm. K. Base of merosporangiospore (MB) with appendage (A) attached to the inner layer of the merosporangiospore cell wall and passing through the septum pore to the pseudophialide neck. Bar = 0.1 μm.

Mentions: Linderina pennispora grows on malt extract agar (MEA), with sporulation occurring at 20 °C within 6–8 d of inoculation. The axenic cultures were yellow, and having two types of hyphae occurred; submerged and aerial. Sporangiophores were erect, “zigzag”-shaped, and produced from submerged hyphae. The asexual apparatus was composed of a non-septate, dome-shaped sporocladium that produced numerous pseudophialides. Each pseudophialide produced a single merosporangium (Fig. 1F).


Development of merosporangia in Linderina pennispora (Kickxellales, Kickxellaceae).

Zain ME, Moss ST, El-Sheikh HH - IMA Fungus (2012)

Scanning electron (A, B, D, F–I) and transmission micrographs (C, E, J, K) of sporulating structures of Linderina pennispora. A. Immature, globose sporocladia formed apically on the sporangiophore (Sp). Bars = 5 μm. B. Pseudophialide initials formed on sporocladia (S). Bars = 5 μm. C. Longitudinal sections of a sporocladium (S) with pseudophialides initials. Bar = 1 μm. D. Ellipsoid pseudophialides (Ps) with merosporangium initials. S = Sporocladium, Sp = Sorangiophores. Bar = 5 μm. E. Longitudinal sections through the distal region of the pseudophialide (Ps), the septum between a pseudophialide neck (PsN) and merosporangium (M). The pore in the septal cross wall (CW) contains a biconvex septal-plug (P), and the base of the merosporangiospore (Ms) is constricted towards the septum. P = septal-plug. Bar = 0.25 μm (c). F. Mature sporocladium (S), pseudophialides (Ps) and merosporangia (M). Bar = 5 μm. G. Obovate merosporangia (M) on pseudophialides (Ps). Note the regular annulations on the merosporangia surface, and the septum on the pseudophialide neck (N). Bar = 5 μm. H. Merosporangium and merosporangiospore cell wall. Three wall-layered of merosporangium (MW) and four-layered wall of the merosporangiospore (MsW). Bar = 0.1 μm. I. Released merosporangia (M) with a single, basally-attached “appendage”. Bar = 2 μm. J. Ellipsoid pseudophialides (Ps) coated with rod-shape ornamentations. Bar = 2 μm. K. Base of merosporangiospore (MB) with appendage (A) attached to the inner layer of the merosporangiospore cell wall and passing through the septum pore to the pseudophialide neck. Bar = 0.1 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3539312&req=5

Figure 1: Scanning electron (A, B, D, F–I) and transmission micrographs (C, E, J, K) of sporulating structures of Linderina pennispora. A. Immature, globose sporocladia formed apically on the sporangiophore (Sp). Bars = 5 μm. B. Pseudophialide initials formed on sporocladia (S). Bars = 5 μm. C. Longitudinal sections of a sporocladium (S) with pseudophialides initials. Bar = 1 μm. D. Ellipsoid pseudophialides (Ps) with merosporangium initials. S = Sporocladium, Sp = Sorangiophores. Bar = 5 μm. E. Longitudinal sections through the distal region of the pseudophialide (Ps), the septum between a pseudophialide neck (PsN) and merosporangium (M). The pore in the septal cross wall (CW) contains a biconvex septal-plug (P), and the base of the merosporangiospore (Ms) is constricted towards the septum. P = septal-plug. Bar = 0.25 μm (c). F. Mature sporocladium (S), pseudophialides (Ps) and merosporangia (M). Bar = 5 μm. G. Obovate merosporangia (M) on pseudophialides (Ps). Note the regular annulations on the merosporangia surface, and the septum on the pseudophialide neck (N). Bar = 5 μm. H. Merosporangium and merosporangiospore cell wall. Three wall-layered of merosporangium (MW) and four-layered wall of the merosporangiospore (MsW). Bar = 0.1 μm. I. Released merosporangia (M) with a single, basally-attached “appendage”. Bar = 2 μm. J. Ellipsoid pseudophialides (Ps) coated with rod-shape ornamentations. Bar = 2 μm. K. Base of merosporangiospore (MB) with appendage (A) attached to the inner layer of the merosporangiospore cell wall and passing through the septum pore to the pseudophialide neck. Bar = 0.1 μm.
Mentions: Linderina pennispora grows on malt extract agar (MEA), with sporulation occurring at 20 °C within 6–8 d of inoculation. The axenic cultures were yellow, and having two types of hyphae occurred; submerged and aerial. Sporangiophores were erect, “zigzag”-shaped, and produced from submerged hyphae. The asexual apparatus was composed of a non-septate, dome-shaped sporocladium that produced numerous pseudophialides. Each pseudophialide produced a single merosporangium (Fig. 1F).

Bottom Line: The sporangiophore was erect, cylindrical, and narrower (4-8 μm) than the vegetative mycelium (8-12 μm).The pseudophialides had a round, ca. 1.5 μm diam, base with a narrower, 0.7-0.8 μm diam lobed, cylindrical neck structure in the distal region which extended to the pseudophialide neck.Detached merosporangia had a single, acicular, unbranched, 3-5 μm long, ca. 0.1 μm diam "appendage" that was attached to the merosporangiospore inner cell wall layer and passed through the septum plug to the pseudophialide.

View Article: PubMed Central - PubMed

Affiliation: Center of Excellence in Biotechnology Research, King Saud University, Riyadh, Saudi Arabia; ; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo, Egypt.

ABSTRACT
The vegetative and sporulating structures of Linderina pennispora are described using scanning and transmission electron microscopy. The vegetative hyphae and sporangiophores were regularly septate, possessed a two-layered wall, and coated with rod-shaped, 0.2-0.7 μm long, 0.15-0.25 μm wide ornamentations. The sporangiophore was erect, cylindrical, and narrower (4-8 μm) than the vegetative mycelium (8-12 μm). The mature sporocladium was ovoid to dome-shaped, sessile, non-septate, 18-24 μm diam, possessed a two-layered wall, and coated with rod-shaped ornamentations. Mature pseudophialides were ellipsoid, 2.0-2.5 μm wide, 4-7 μm long, possessed a two-layered wall, and formed in a series of concentric groups radiating from the "apex" of the sporocladium. The pseudophialides had a round, ca. 1.5 μm diam, base with a narrower, 0.7-0.8 μm diam lobed, cylindrical neck structure in the distal region which extended to the pseudophialide neck. The merosporangia were obovate, 3-4 μm wide near the base, and narrowed distally to 2.0-2.5 μm wide, 18-23 μm long, possessed a three-layered wall, with regular surface annulation with interconnecting ridges, but lacked rod-shaped ornamentations. The merosporangia contained a single, obovate, 2.3-2.5 μm diam merosporangiospore, with a ca. 1 μm diam papilla-like base, that possessed a four-layered wall. Detached merosporangia had a single, acicular, unbranched, 3-5 μm long, ca. 0.1 μm diam "appendage" that was attached to the merosporangiospore inner cell wall layer and passed through the septum plug to the pseudophialide.

No MeSH data available.


Related in: MedlinePlus