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Effect of berberine on depression- and anxiety-like behaviors and activation of the noradrenergic system induced by development of morphine dependence in rats.

Lee B, Sur B, Yeom M, Shim I, Lee H, Hahm DH - Korean J. Physiol. Pharmacol. (2012)

Bottom Line: Daily BER administration significantly decreased immobility in the forced swimming test and increased open-arm exploration in the elevated plus maze test.BER administration also significantly blocked the increase in hypothalamic CRF expression and TH expression in the locus coeruleus (LC) and the decrease in hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression.Taken together, these findings demonstrated that BER administration significantly reduced morphine withdrawal-associated behaviors following discontinuation of repeated morphine administration in rats, possibly through modulation of hypothalamic CRF and the central noradrenergic system.

View Article: PubMed Central - PubMed

Affiliation: Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea.

ABSTRACT
The purpose of this study was to evaluate whether berberine (BER) administration could attenuate depression- and anxiety-like behaviors and increase corticotrophin-releasing factor (CRF) and tyrosine hydroxylase (TH) expression following chronic morphine withdrawal in rats. Male rats were exposed to chronic, intermittent, escalating morphine (10~50 mg/kg) for 10 days. After the last morphine injection, depression- and anxiety-like beahvior associated with morphine discontinuation persisted for at least three days during withdrawal without any change in ambulatory activity. Daily BER administration significantly decreased immobility in the forced swimming test and increased open-arm exploration in the elevated plus maze test. BER administration also significantly blocked the increase in hypothalamic CRF expression and TH expression in the locus coeruleus (LC) and the decrease in hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression. Taken together, these findings demonstrated that BER administration significantly reduced morphine withdrawal-associated behaviors following discontinuation of repeated morphine administration in rats, possibly through modulation of hypothalamic CRF and the central noradrenergic system. BER may be a useful agent for treating or alleviating complex withdrawal symptoms and preventing morphine use relapses.

No MeSH data available.


Related in: MedlinePlus

RT-PCR bands (A) and their relative intensities (B) of brain-derived neurotrophic factor (BDNF) mRNA in the rat hippocampus after withdrawal from repeated saline or morphine administration. **p<0.01 vs. SAL group; #p<0.05, ##p<0.01 vs. MOR group.
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Figure 5: RT-PCR bands (A) and their relative intensities (B) of brain-derived neurotrophic factor (BDNF) mRNA in the rat hippocampus after withdrawal from repeated saline or morphine administration. **p<0.01 vs. SAL group; #p<0.05, ##p<0.01 vs. MOR group.

Mentions: The effect of BER administration on BDNF mRNA expression was investigated in the rat hippocampus following withdrawal from repeated morphine injection using RT-PCR analysis (Fig. 5). BDNF mRNA expression was normalized against glyceraldehydes-3-phophate dehydrogenase (GAPDH) mRNA, a housekeeping gene used as an internal control. BDNF mRNA expression in the MOR group hippocampus significantly increased compared with that in the SAL group (p<0.01). The increased BDNF mRNA expression in the MOR groups was significantly restored in the BER50 group (p<0.05). The recovery of the expression levels of BNDF mRNA in the BER50 group was comparable to that in the FLX group.


Effect of berberine on depression- and anxiety-like behaviors and activation of the noradrenergic system induced by development of morphine dependence in rats.

Lee B, Sur B, Yeom M, Shim I, Lee H, Hahm DH - Korean J. Physiol. Pharmacol. (2012)

RT-PCR bands (A) and their relative intensities (B) of brain-derived neurotrophic factor (BDNF) mRNA in the rat hippocampus after withdrawal from repeated saline or morphine administration. **p<0.01 vs. SAL group; #p<0.05, ##p<0.01 vs. MOR group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3526741&req=5

Figure 5: RT-PCR bands (A) and their relative intensities (B) of brain-derived neurotrophic factor (BDNF) mRNA in the rat hippocampus after withdrawal from repeated saline or morphine administration. **p<0.01 vs. SAL group; #p<0.05, ##p<0.01 vs. MOR group.
Mentions: The effect of BER administration on BDNF mRNA expression was investigated in the rat hippocampus following withdrawal from repeated morphine injection using RT-PCR analysis (Fig. 5). BDNF mRNA expression was normalized against glyceraldehydes-3-phophate dehydrogenase (GAPDH) mRNA, a housekeeping gene used as an internal control. BDNF mRNA expression in the MOR group hippocampus significantly increased compared with that in the SAL group (p<0.01). The increased BDNF mRNA expression in the MOR groups was significantly restored in the BER50 group (p<0.05). The recovery of the expression levels of BNDF mRNA in the BER50 group was comparable to that in the FLX group.

Bottom Line: Daily BER administration significantly decreased immobility in the forced swimming test and increased open-arm exploration in the elevated plus maze test.BER administration also significantly blocked the increase in hypothalamic CRF expression and TH expression in the locus coeruleus (LC) and the decrease in hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression.Taken together, these findings demonstrated that BER administration significantly reduced morphine withdrawal-associated behaviors following discontinuation of repeated morphine administration in rats, possibly through modulation of hypothalamic CRF and the central noradrenergic system.

View Article: PubMed Central - PubMed

Affiliation: Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University, Seoul 130-701, Korea.

ABSTRACT
The purpose of this study was to evaluate whether berberine (BER) administration could attenuate depression- and anxiety-like behaviors and increase corticotrophin-releasing factor (CRF) and tyrosine hydroxylase (TH) expression following chronic morphine withdrawal in rats. Male rats were exposed to chronic, intermittent, escalating morphine (10~50 mg/kg) for 10 days. After the last morphine injection, depression- and anxiety-like beahvior associated with morphine discontinuation persisted for at least three days during withdrawal without any change in ambulatory activity. Daily BER administration significantly decreased immobility in the forced swimming test and increased open-arm exploration in the elevated plus maze test. BER administration also significantly blocked the increase in hypothalamic CRF expression and TH expression in the locus coeruleus (LC) and the decrease in hippocampal brain-derived neurotrophic factor (BDNF) mRNA expression. Taken together, these findings demonstrated that BER administration significantly reduced morphine withdrawal-associated behaviors following discontinuation of repeated morphine administration in rats, possibly through modulation of hypothalamic CRF and the central noradrenergic system. BER may be a useful agent for treating or alleviating complex withdrawal symptoms and preventing morphine use relapses.

No MeSH data available.


Related in: MedlinePlus