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Cloning of the quail PIWI gene and characterization of PIWI binding to small RNAs.

Chen R, Chang G, Zhang Y, Dai A, Ma T, Li J, Zhai F, Hua D, Xia M, Chen G - PLoS ONE (2012)

Bottom Line: The present study cloned and analyzed the sequences of the PIWIL1 protein.Small RNAs showed a strong peak at 24-27 nt in the testicular RNA library, mapped primarily to repeat sequences and were similar to rasiRNAs.MicroRNAs (miRNAs) were abundant in the ovarian RNA library at a peak of 22 nt.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

ABSTRACT
The PIWI protein regulates gene expression at the epigenetic and post-transcriptional level with a variety of endogenous small non-coding RNAs. In poultry, the biological function of the PIWI protein and PIWI binding to small RNAs had not been determined. The present study cloned and analyzed the sequences of the PIWIL1 protein. We also characterized PIWIL1 binding to small RNAs from adult quail testis, where the PIWIL1 protein is specifically expressed. Small RNAs showed a strong peak at 24-27 nt in the testicular RNA library, mapped primarily to repeat sequences and were similar to rasiRNAs. MicroRNAs (miRNAs) were abundant in the ovarian RNA library at a peak of 22 nt.

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PIWIL1 is specifically expressed in the testis.A: Northern blot analysis of PIWIL1 expression in adult tissues with β-actin as the loading control. B: Western blot analysis of adult tissues; H, heart; L, live; K, kidney; B, brain; T, testis; O, ovary; F, lung; XJ, pectoral muscle.
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pone-0051724-g002: PIWIL1 is specifically expressed in the testis.A: Northern blot analysis of PIWIL1 expression in adult tissues with β-actin as the loading control. B: Western blot analysis of adult tissues; H, heart; L, live; K, kidney; B, brain; T, testis; O, ovary; F, lung; XJ, pectoral muscle.

Mentions: To explore the role of PIWIL1 in development, we examined its expression in major adult organs by Northern blotting (Figure 2A). The PIWIL1 gene encoded a 4.4 kb transcript that was specifically expressed in the adult testis. The adult- and testis-specific expression of PIWIL1 was further confirmed by Western blotting (Figures 2B and S1). As expected, a 98 kDa protein fragment was detected in the adult testis. And it was indicated that our anti-PIWIL1 antibody could recognize the PIWIL1 protein from the testicular extract.


Cloning of the quail PIWI gene and characterization of PIWI binding to small RNAs.

Chen R, Chang G, Zhang Y, Dai A, Ma T, Li J, Zhai F, Hua D, Xia M, Chen G - PLoS ONE (2012)

PIWIL1 is specifically expressed in the testis.A: Northern blot analysis of PIWIL1 expression in adult tissues with β-actin as the loading control. B: Western blot analysis of adult tissues; H, heart; L, live; K, kidney; B, brain; T, testis; O, ovary; F, lung; XJ, pectoral muscle.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526641&req=5

pone-0051724-g002: PIWIL1 is specifically expressed in the testis.A: Northern blot analysis of PIWIL1 expression in adult tissues with β-actin as the loading control. B: Western blot analysis of adult tissues; H, heart; L, live; K, kidney; B, brain; T, testis; O, ovary; F, lung; XJ, pectoral muscle.
Mentions: To explore the role of PIWIL1 in development, we examined its expression in major adult organs by Northern blotting (Figure 2A). The PIWIL1 gene encoded a 4.4 kb transcript that was specifically expressed in the adult testis. The adult- and testis-specific expression of PIWIL1 was further confirmed by Western blotting (Figures 2B and S1). As expected, a 98 kDa protein fragment was detected in the adult testis. And it was indicated that our anti-PIWIL1 antibody could recognize the PIWIL1 protein from the testicular extract.

Bottom Line: The present study cloned and analyzed the sequences of the PIWIL1 protein.Small RNAs showed a strong peak at 24-27 nt in the testicular RNA library, mapped primarily to repeat sequences and were similar to rasiRNAs.MicroRNAs (miRNAs) were abundant in the ovarian RNA library at a peak of 22 nt.

View Article: PubMed Central - PubMed

Affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, China.

ABSTRACT
The PIWI protein regulates gene expression at the epigenetic and post-transcriptional level with a variety of endogenous small non-coding RNAs. In poultry, the biological function of the PIWI protein and PIWI binding to small RNAs had not been determined. The present study cloned and analyzed the sequences of the PIWIL1 protein. We also characterized PIWIL1 binding to small RNAs from adult quail testis, where the PIWIL1 protein is specifically expressed. Small RNAs showed a strong peak at 24-27 nt in the testicular RNA library, mapped primarily to repeat sequences and were similar to rasiRNAs. MicroRNAs (miRNAs) were abundant in the ovarian RNA library at a peak of 22 nt.

Show MeSH
Related in: MedlinePlus