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Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions.

Dukic-Stefanovic S, Walther J, Wosch S, Zimmermann G, Wiedemann P, Alexander H, Claudepierre T - PLoS ONE (2012)

Bottom Line: Here we demonstrated that human retina is producing this gonadotropin that acts as a neuroactive molecule.Müller glial and retinal pigmented epithelial (RPE) cells are producing hCG that may affects neighbour cells expressing its receptor, namely cone photoreceptors.The exact physiological functions of this circuit have still to be identified, but the proliferation of photoreceptor-derived tumor induced by hCG demonstrated the need to control this neuroendocrine loop.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Medicine, University of Leipzig, Leipzig, Germany.

ABSTRACT
Extra-gonadal role of gonadotropins has been re-evaluated over the last 20 years. In addition to pituitary secretion of luteinizing hormone (LH) and follicle stimulating hormone (FSH), the CNS has been clearly identified as a source of hCG acting locally to influence behaviour. Here we demonstrated that human retina is producing this gonadotropin that acts as a neuroactive molecule. Müller glial and retinal pigmented epithelial (RPE) cells are producing hCG that may affects neighbour cells expressing its receptor, namely cone photoreceptors. It was previously described that amacrine and retinal ganglion (RGC) cells are targets of the gonadotropin releasing hormone that control the secretion of all gonadotropins. Therefore our findings suggest that a complex neuroendocrine circuit exists in the retina, involving hCG secreting cells (glial and RPE), hCG targets (photoreceptors) and hCG-release controlling cells (amacrine and RGC). The exact physiological functions of this circuit have still to be identified, but the proliferation of photoreceptor-derived tumor induced by hCG demonstrated the need to control this neuroendocrine loop.

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Related in: MedlinePlus

Human retinoblastoma cells express LHR.Gonadotropin receptor LHR was found expressed at retinoblastoma surface. Punctuate signal was enhance and around cell soma and at cell contact (arrows in A, C) while nucleus was not stained (asterisk in A, C) Fillopodia and lamelipodia extensions of retinoblastoma, heavily stained with phalloidin (B) were not labeled for LHR. Clusters of LHR-positive structures may account for membrane pieces of dead/detached retinoblastoma cells in the culture (arrowhead in A, C). Scale bar 5 µm. A: Rabbit polyclonal anti LHR (Santa Cruz biotechnology ref# ab25828) use 1/100 in PBS, 0.1% BSA, 0.01% triton x100; B : Phalloidin-488 (Millipore ref# A12379) use at 1/50 in PBS; C : Merged image, with blue channel corresponding to DAPI nucleus staining used at 1/1000.
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pone-0052567-g004: Human retinoblastoma cells express LHR.Gonadotropin receptor LHR was found expressed at retinoblastoma surface. Punctuate signal was enhance and around cell soma and at cell contact (arrows in A, C) while nucleus was not stained (asterisk in A, C) Fillopodia and lamelipodia extensions of retinoblastoma, heavily stained with phalloidin (B) were not labeled for LHR. Clusters of LHR-positive structures may account for membrane pieces of dead/detached retinoblastoma cells in the culture (arrowhead in A, C). Scale bar 5 µm. A: Rabbit polyclonal anti LHR (Santa Cruz biotechnology ref# ab25828) use 1/100 in PBS, 0.1% BSA, 0.01% triton x100; B : Phalloidin-488 (Millipore ref# A12379) use at 1/50 in PBS; C : Merged image, with blue channel corresponding to DAPI nucleus staining used at 1/1000.

Mentions: Moreover, Y79 Retinoblastoma cells line, a cone precursor tumor [27] was tested for LHR expression and we found the hCG receptor concentrated at the cancer cell membrane (Figure 4).


Chorionic gonadotropin and its receptor are both expressed in human retina, possible implications in normal and pathological conditions.

Dukic-Stefanovic S, Walther J, Wosch S, Zimmermann G, Wiedemann P, Alexander H, Claudepierre T - PLoS ONE (2012)

Human retinoblastoma cells express LHR.Gonadotropin receptor LHR was found expressed at retinoblastoma surface. Punctuate signal was enhance and around cell soma and at cell contact (arrows in A, C) while nucleus was not stained (asterisk in A, C) Fillopodia and lamelipodia extensions of retinoblastoma, heavily stained with phalloidin (B) were not labeled for LHR. Clusters of LHR-positive structures may account for membrane pieces of dead/detached retinoblastoma cells in the culture (arrowhead in A, C). Scale bar 5 µm. A: Rabbit polyclonal anti LHR (Santa Cruz biotechnology ref# ab25828) use 1/100 in PBS, 0.1% BSA, 0.01% triton x100; B : Phalloidin-488 (Millipore ref# A12379) use at 1/50 in PBS; C : Merged image, with blue channel corresponding to DAPI nucleus staining used at 1/1000.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3526580&req=5

pone-0052567-g004: Human retinoblastoma cells express LHR.Gonadotropin receptor LHR was found expressed at retinoblastoma surface. Punctuate signal was enhance and around cell soma and at cell contact (arrows in A, C) while nucleus was not stained (asterisk in A, C) Fillopodia and lamelipodia extensions of retinoblastoma, heavily stained with phalloidin (B) were not labeled for LHR. Clusters of LHR-positive structures may account for membrane pieces of dead/detached retinoblastoma cells in the culture (arrowhead in A, C). Scale bar 5 µm. A: Rabbit polyclonal anti LHR (Santa Cruz biotechnology ref# ab25828) use 1/100 in PBS, 0.1% BSA, 0.01% triton x100; B : Phalloidin-488 (Millipore ref# A12379) use at 1/50 in PBS; C : Merged image, with blue channel corresponding to DAPI nucleus staining used at 1/1000.
Mentions: Moreover, Y79 Retinoblastoma cells line, a cone precursor tumor [27] was tested for LHR expression and we found the hCG receptor concentrated at the cancer cell membrane (Figure 4).

Bottom Line: Here we demonstrated that human retina is producing this gonadotropin that acts as a neuroactive molecule.Müller glial and retinal pigmented epithelial (RPE) cells are producing hCG that may affects neighbour cells expressing its receptor, namely cone photoreceptors.The exact physiological functions of this circuit have still to be identified, but the proliferation of photoreceptor-derived tumor induced by hCG demonstrated the need to control this neuroendocrine loop.

View Article: PubMed Central - PubMed

Affiliation: Department of Ophthalmology, Faculty of Medicine, University of Leipzig, Leipzig, Germany.

ABSTRACT
Extra-gonadal role of gonadotropins has been re-evaluated over the last 20 years. In addition to pituitary secretion of luteinizing hormone (LH) and follicle stimulating hormone (FSH), the CNS has been clearly identified as a source of hCG acting locally to influence behaviour. Here we demonstrated that human retina is producing this gonadotropin that acts as a neuroactive molecule. Müller glial and retinal pigmented epithelial (RPE) cells are producing hCG that may affects neighbour cells expressing its receptor, namely cone photoreceptors. It was previously described that amacrine and retinal ganglion (RGC) cells are targets of the gonadotropin releasing hormone that control the secretion of all gonadotropins. Therefore our findings suggest that a complex neuroendocrine circuit exists in the retina, involving hCG secreting cells (glial and RPE), hCG targets (photoreceptors) and hCG-release controlling cells (amacrine and RGC). The exact physiological functions of this circuit have still to be identified, but the proliferation of photoreceptor-derived tumor induced by hCG demonstrated the need to control this neuroendocrine loop.

Show MeSH
Related in: MedlinePlus